Restriction map for
pFN21AB6601
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccIII AclI AflII AflIII
AhdI Alw44I AocI ApaLI AscI
AseI AsnI Asp700I AspEI AviII
AvrII BamHI BbrPI BciVI BclI
BfaI BfrI BglII BlnI BsaAI
BsaBI BsaI BseAI BsgI BsiWI
BsmI Bsp1407I BspDI BspEI BspLU11I
BsrBI BsrDI BsrGI BsrI Bst1107I
BstAPI BstBI BstF5I BstZ17I Bsu36I
ClaI CpoI DraI DrdI Eam1105I
Eco81I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI HincII HindII
HindIII HpaI KspI MaeI MamI
MfeI MluI MroI MseI MunI
NaeI NdeI NgoMIV NheI NruI
NsiI NspV PacI PaeR7I PmaCI
PmeI PmlI PshAI PshBI Psp1406I
PstI PvuI RsrII SacI SacII
SalI SbfI ScaI SexAI SfaNI
SfcI SfuI SgrAI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tru9I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 62 enzymes) #
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Acc65I 244 GGTACC G^GTACC
AccI 673 GTCTAC GT^[AC][GT]AC
AcsI 1147 GAATTT [GA]^AATT[CT]
AgeI 241 ACCGGT A^CCGGT
Aor51HI 386 AGCGCT AGC^GCT
ApaI 25 GGGCCC GGGCC^C
ApoI 1147 GAATTT [GA]^AATT[CT]
Asp718I 244 GGTACC G^GTACC
AspHI 943 GTGCTC G[AT]GC[AT]^C
AspI 983 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbeI 562 GGCGCC GGCGC^C
BbsI 787 GTCTTC GAAGAC(2/6)
BcgI 1065 CGATTCTCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 733 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 733 GCTCAGC GC^T[ACGT]AGC
BpuAI 787 GTCTTC GAAGAC(2/6)
BsaWI 241 ACCGGT [AT]^CCGG[AT]
BsiHKAI 943 GTGCTC G[AT]GC[AT]^C
BspHI 332 TCATGA T^CATGA
BspMI 125 ACCTGC ACCTGC(4/8)
BsrFI 241 ACCGGT [GA]^CCGG[CT]
BssHII 152 GCGCGC G^CGCGC
BssSI 1070 CTCGTG CACGAG(-5/-1)
BstEII 237 GGTGACC G^GT[ACGT]ACC
BstPI 237 GGTGACC G^GT[ACGT]ACC
BstXI 122 CCAACCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 902 AGATCC [GA]^GATC[CT]
CelII 733 GCTCAGC GC^T[ACGT]AGC
Cfr10I 241 ACCGGT [GA]^CCGG[CT]
EagI 473 CGGCCG C^GGCCG
EclXI 473 CGGCCG C^GGCCG
Eco47III 386 AGCGCT AGC^GCT
Eco52I 473 CGGCCG C^GGCCG
EcoNI 1189 CCTCCAACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 237 GGTGACC G^GT[ACGT]ACC
EcoT14I 374 CCATGG C^C[AT][AT]GG
KasI 562 GGCGCC G^GCGCC
KpnI 244 GGTACC GGTAC^C
MaeIII 238 GTGAC ^GT[ACGT]AC
MflI 902 AGATCC [GA]^GATC[CT]
NarI 562 GGCGCC GG^CGCC
NcoI 374 CCATGG C^CATGG
NotI 472 GCGGCCGC GC^GGCCGC
NspI 1130 GCATGC [GA]CATG^[CT]
PflFI 983 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 900 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 241 ACCGGT A^CCGGT
PpuMI 538 GGGTCCT [GA]G^G[AT]CC[CT]
RcaI 332 TCATGA T^CATGA
SfiI 369 GGCCGCCATGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 562 GGCGCC GGC^GCC
SphI 1130 GCATGC GCATG^C
StyI 374 CCATGG C^C[AT][AT]GG
TaqI 278 TCGA T^CGA
TfiI 1066 GATTC G^A[AT]TC
Tsp45I 238 GTGAC ^GT[GC]AC
Tsp509I 1148 AATT ^AATT
Tth111I 983 GACGGAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 278 TCGA T^CGA
Van91I 900 CCAGATCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 452 CCACCGTGGGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 902 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 13 enzymes) #
###################################
AlwI 903 GATCC GGATC(4/5)
746 GGATC
AlwNI 736 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
901 CAGATCCTG
BanI 244 GGTACC G^G[CT][GA]CC
562 GGCGCC
BanII 25 GGGCCC G[GA]GC[CT]^C
828 GAGCCC
BbvCI 966 CCTCAGC CCTCAGC(-5/-2)
1076 CCTCAGC
BglI 370 GCCGCCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
475 GCCGCGCTGGC
BseRI 180 CTCCTC GAGGAG(10/8)
58 GAGGAG
EarI 274 CTCTTC CTCTTC(1/4)
595 CTCTTC
Ksp632I 274 CTCTTC CTCTTC(1/4)
595 CTCTTC
MaeII 578 ACGT A^CGT
671 ACGT
MslI 249 CATCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
750 CATGGTGGTG
PvuII 841 CAGCTG CAG^CTG
1091 CAGCTG
SapI 273 GCTCTTC GCTCTTC(1/4)
594 GCTCTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 73 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AvaI AvaII BalI BbvI BcnI
BmyI BpmI BsaHI BsaJI BsiEI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BssKI BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EaeI EcoO109I
EcoRII FauI Fnu4HI HaeII HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MboI
MboII MluNI MnlI MscI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV PleI RsaI
Sau3AI Sau96I ScrFI SmaI TseI
TspRI XmaCI XmaI