Restriction map for
pFN21AE1915
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
Acc65I AccIII AclI AflII AflIII
AhdI AocI Aor51HI ApaI AscI
AseI AsnI Asp718I AspEI AspI
AvaI AviII AvrII BalI BbsI
BbvCI BciVI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BpuAI BsaBI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrGI BssHII
Bst1107I BstBI BstEII BstPI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
FbaI FseI FspI HpaI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MslI MunI NaeI
NciI NcoI NdeI NgoMIV NotI
NruI NspV PacI PaeR7I PflFI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SapI SbfI SexAI
SfiI SfuI SmaI SnaBI SpeI
Sse8387I SspBI SspI SwaI TfiI
Tth111I XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 50 enzymes) #
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AatII 715 GACGTC GACGT^C
AccI 468 GTCGAC GT^[AC][GT]AC
AgeI 1280 ACCGGT A^CCGGT
Alw44I 622 GTGCAC G^TGCAC
AlwNI 473 CAGATACTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 622 GTGCAC G^TGCAC
Asp700I 172 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1015 GGATCC G^GATCC
BanII 214 GAGCTC G[GA]GC[CT]^C
BbrPI 1204 CACGTG CAC^GTG
BsaAI 1204 CACGTG [CT]AC^GT[GA]
BsaI 322 GAGACC GGTCTC(1/5)
BspMI 783 ACCTGC ACCTGC(4/8)
BsrBI 1186 GAGCGG CCGCTC(-3/-3)
BsrDI 617 GCAATG GCAATG(2/0)
BssSI 593 CTCGTG CACGAG(-5/-1)
BstAPI 1310 GCACTCTGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 990 CCAACAAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1015 GGATCC [GA]^GATC[CT]
DraII 282 GGGTCCC [GA]G^G[ACGT]CC[CT]
DraIII 1311 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 960 CCGTGG C^C[GA][CT]GG
EarI 694 CTCTTC CTCTTC(1/4)
EcoO109I 282 GGGTCCC [GA]G^G[ACGT]CC[CT]
EcoRI 1273 GAATTC G^AATTC
EcoT14I 200 CCTTGG C^C[AT][AT]GG
EcoT22I 94 ATGCAT ATGCA^T
HgaI 68 GACGC GACGC(5/10)
Ksp632I 694 CTCTTC CTCTTC(1/4)
MflI 1015 GGATCC [GA]^GATC[CT]
MseI 1223 TTAA T^TAA
NheI 138 GCTAGC G^CTAGC
NsiI 94 ATGCAT ATGCA^T
PflMI 246 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 1280 ACCGGT A^CCGGT
PmaCI 1204 CACGTG CAC^GTG
PmlI 1204 CACGTG CAC^GTG
PpuMI 282 GGGTCCC [GA]G^G[AT]CC[CT]
PshAI 409 GACTTGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
SacI 214 GAGCTC GAGCT^C
SalI 468 GTCGAC G^TCGAC
ScaI 679 AGTACT AGT^ACT
SgrAI 1279 CACCGGTG C[GA]^CCGG[CT]G
SphI 92 GCATGC GCATG^C
StuI 953 AGGCCT AGG^CCT
StyI 200 CCTTGG C^C[AT][AT]GG
Tru9I 1223 TTAA T^TAA
Van91I 246 CCAGAATGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1015 GGATCC [GA]^GATC[CT]
XmnI 172 GAACATCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 15 CGCG CG^CG
1079 CGCG
AspHI 214 GAGCTC G[AT]GC[AT]^C
622 GTGCAC
BanI 6 GGCGCC G^G[CT][GA]CC
841 GGCGCC
BbeI 6 GGCGCC GGCGC^C
841 GGCGCC
BbvI 1307 GCAGC GCAGC(8/12)
270 GCTGC
BcgI 1161 CGAGGCTCTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
817 GCACTCCTCTCG
BmyI 214 GAGCTC G[AGT]GC[ACT]^C
622 GTGCAC
BsaWI 426 TCCGGT [AT]^CCGG[AT]
1280 ACCGGT
BsgI 740 CTGCAC GTGCAG(16/14)
785 CTGCAC
BsiHKAI 214 GAGCTC G[AT]GC[AT]^C
622 GTGCAC
Bsp1286I 214 GAGCTC G[AGT]GC[ACT]^C
622 GTGCAC
BsrFI 1042 GCCGGT [GA]^CCGG[CT]
1280 ACCGGT
BstUI 15 CGCG CG^CG
1079 CGCG
Cfr10I 1042 GCCGGT [GA]^CCGG[CT]
1280 ACCGGT
EaeI 53 TGGCCG [CT]^GGCC[GA]
1208 TGGCCG
FauI 16 GCGGG CCCGC(4/6)
1188 GCGGG
HaeII 6 GGCGCC [GA]GCGC^[CT]
841 GGCGCC
HincII 468 GTCGAC GT[CT]^[GA]AC
985 GTTGAC
HindII 468 GTCGAC GT[CT]^[GA]AC
985 GTTGAC
HindIII 290 AAGCTT A^AGCTT
700 AAGCTT
KasI 6 GGCGCC G^GCGCC
841 GGCGCC
MaeII 716 ACGT A^CGT
1205 ACGT
MvnI 15 CGCG CG^CG
1079 CGCG
NarI 6 GGCGCC GG^CGCC
841 GGCGCC
PstI 763 CTGCAG CTGCA^G
1305 CTGCAG
PvuII 148 CAGCTG CAG^CTG
565 CAGCTG
SfoI 6 GGCGCC GGC^GCC
841 GGCGCC
SmlI 411 CTTGAG C^T[CT][GA]AG
1172 CTCAAG
TaqI 469 TCGA T^CGA
1160 TCGA
TseI 270 GCTGC G^C[AT]GC
1307 GCAGC
TthHB8I 469 TCGA T^CGA
1160 TCGA
XcmI 845 CCATCTATGGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
951 CCAGGCCTGCCGTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI ApoI AvaII BfaI BpmI
BsaHI BsaJI BseRI BsiYI BslI
BsmAI BsmFI BsrI BssKI BstF5I
BstNI Cac8I CfoI Cfr13I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HhaI Hin1I HinP1I HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MspA1I MspI
MvaI MwoI NdeII NlaIII NlaIV
NspI PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI Tsp45I Tsp509I
TspRI