Restriction map for
pFN21AE1719
##################################
# NO CUT ENZYMES ( 144 enzymes) #
##################################
AatII AccI AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwNI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I AspEI AspHI AspI AviII
AvrII BamHI BbeI BbrPI BcgI
BclI BfaI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstAPI
BstBI BstYI BstZ17I Bsu36I CelII
ClaI DraI DraII DraIII DrdI
Eam1105I EarI Eco47III Eco81I EcoO109I
EcoRI EcoRV EcoT22I FbaI FseI
FspI Hin1I HincII HindII HindIII
HpaI KasI Ksp632I MaeI MamI
MfeI MflI MluI MroI MunI
NarI NcoI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI SacI
SalI SapI SbfI ScaI SexAI
SfoI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TaqI Tth111I TthHB8I Van91I
XbaI XhoI XhoII XmnI
###################################
# ONE CUT ENZYMES ( 36 enzymes) #
###################################
Acc65I 512 GGTACC G^GTACC
Asp718I 512 GGTACC G^GTACC
BanII 49 GAGCCC G[GA]GC[CT]^C
BbsI 564 GAAGAC GAAGAC(2/6)
BciVI 288 GGATAC GTATCC(6/5)
BglI 168 GCCCGCGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 564 GAAGAC GAAGAC(2/6)
BseRI 127 CTCCTC GAGGAG(10/8)
BsmI 381 GAATGC GAATGC(1/-1)
BspHI 542 TCATGA T^CATGA
BstEII 339 GGTCACC G^GT[ACGT]ACC
BstPI 339 GGTCACC G^GT[ACGT]ACC
BstXI 593 CCAAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CpoI 179 CGGACCG CG^G[AT]CCG
EcoNI 522 CCTGGACAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 339 GGTCACC G^GT[ACGT]ACC
HgaI 492 GCGTC GACGC(5/10)
HinfI 255 GAATC G^A[ACGT]TC
KpnI 512 GGTACC GGTAC^C
MaeII 461 ACGT A^CGT
MboII 564 GAAGA GAAGA(8/7)
MslI 372 CATGGCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 62 GCCGGC GCC^GGC
NgoMIV 62 GCCGGC G^CCGGC
RcaI 542 TCATGA T^CATGA
RsrII 179 CGGACCG CG^G[AT]CCG
SfcI 399 CTACAG C^T[GA][CT]AG
SfiI 167 GGCCCGCGCGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SmaI 52 CCCGGG CCC^GGG
StuI 647 AGGCCT AGG^CCT
TfiI 255 GAATC G^A[AT]TC
Tsp509I 454 AATT ^AATT
TspRI 636 CAGTG CA[GC]TG[ACGT][ACGT]^
XcmI 489 CCAGCGTCGTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 52 CCCGGG C^CCGGG
XmaI 52 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AlwI 24 GATCC GGATC(4/5)
318 GATCC
AvaI 52 CCCGGG C^[CT]CG[GA]G
643 CCCGAG
AvaII 42 GGTCC G^G[AT]CC
180 GGACC
BbvCI 196 CCTCAGC CCTCAGC(-5/-2)
385 GCTGAGG
BmyI 49 GAGCCC G[AGT]GC[ACT]^C
276 GGGCAC
BpmI 247 CTCCAG CTGGAG(16/14)
601 CTGGAG
BsiEI 64 CGGCCG CG[GA][CT]^CG
175 CGGCCG
BsoBI 52 CCCGGG C^[CT]CG[GA]G
643 CCCGAG
Bsp1286I 49 GAGCCC G[AGT]GC[ACT]^C
276 GGGCAC
BsrFI 62 GCCGGC [GA]^CCGG[CT]
66 GCCGGT
BsrI 14 CCAGT ACTGG(1/-1)
659 CCAGT
Cfr10I 62 GCCGGC [GA]^CCGG[CT]
66 GCCGGT
DpnII 24 GATC ^GATC
318 GATC
DsaI 96 CCGCGG C^C[GA][CT]GG
183 CCGCGG
EagI 64 CGGCCG C^GGCCG
175 CGGCCG
EclXI 64 CGGCCG C^GGCCG
175 CGGCCG
Eco52I 64 CGGCCG C^GGCCG
175 CGGCCG
EcoT14I 143 CCAAGG C^C[AT][AT]GG
569 CCAAGG
HaeII 6 GGCGCT [GA]GCGC^[CT]
352 AGCGCC
KspI 96 CCGCGG CCGC^GG
183 CCGCGG
MboI 24 GATC ^GATC
318 GATC
MseI 434 TTAA T^TAA
560 TTAA
NdeII 24 GATC ^GATC
318 GATC
PvuII 419 CAGCTG CAG^CTG
575 CAGCTG
SacII 96 CCGCGG CCGC^GG
183 CCGCGG
Sau3AI 24 GATC ^GATC
318 GATC
SfaNI 209 GCATC GCATC(5/9)
269 GCATC
StyI 143 CCAAGG C^C[AT][AT]GG
569 CCAAGG
Tru9I 434 TTAA T^TAA
560 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AfaI AluI BalI
BanI BbvI BcnI BsaJI BsiYI
BslI BsmFI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr13I DdeI
EaeI EcoRII FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HpaII
HphI ItaI MaeIII MluNI MnlI
MscI MspA1I MspI MvaI MvnI
MwoI NciI NlaIII NlaIV RsaI
Sau96I ScrFI TseI Tsp45I