Restriction map for
pFN21AE1138
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BamHI BbeI BbrPI BbsI BbvCI
BcgI BciVI BclI BfaI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseAI BsgI BsiEI BsiWI BsmBI
BsoBI Bsp1407I BspEI BspHI BspLU11I
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstYI BstZ17I CelII Cfr10I
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I EcoO65I EcoRV
EcoT22I FauI FbaI FseI FspI
HindIII HpaI KasI KpnI MaeI
MamI MflI MluI MluNI MroI
MscI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI RcaI RsrII
SalI SapI SbfI SexAI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tsp45I TspRI
Tth111I Van91I XbaI XcmI XhoI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AcsI 826 GAATTC [GA]^AATT[CT]
AlwNI 85 CAGCGTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 826 GAATTC [GA]^AATT[CT]
BanI 360 GGTGCC G^G[CT][GA]CC
BanII 187 GAGCTC G[GA]GC[CT]^C
BcnI 20 CCCGG CC^[GC]GG
BsmAI 1083 GTCTC GTCTC(1/5)
BsmI 1041 GAATGC GAATGC(1/-1)
BspDI 922 ATCGAT AT^CGAT
BspMI 846 GCAGGT ACCTGC(4/8)
BsrBI 547 CCGCTC CCGCTC(-3/-3)
ClaI 922 ATCGAT AT^CGAT
DraII 647 AGGCCCC [GA]G^G[ACGT]CC[CT]
DrdI 328 GACCAAGATGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
DsaI 725 CCGCGG C^C[GA][CT]GG
EaeI 236 TGGCCG [CT]^GGCC[GA]
EcoNI 661 CCTCATTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 647 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoRI 826 GAATTC G^AATTC
HincII 850 GTCAAC GT[CT]^[GA]AC
HindII 850 GTCAAC GT[CT]^[GA]AC
HphI 1098 GGTGA GGTGA(8/7)
KspI 725 CCGCGG CCGC^GG
MaeII 206 ACGT A^CGT
MfeI 674 CAATTG C^AATTG
MunI 674 CAATTG C^AATTG
NciI 20 CCCGG CC^[GC]GG
NruI 125 TCGCGA TCG^CGA
PshAI 201 GACTCACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1021 CTGCAG CTGCA^G
PvuII 148 CAGCTG CAG^CTG
SacI 187 GAGCTC GAGCT^C
SacII 725 CCGCGG CCGC^GG
ScaI 631 AGTACT AGT^ACT
TaqI 923 TCGA T^CGA
TthHB8I 923 TCGA T^CGA
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# TWO CUT ENZYMES ( 24 enzymes) #
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AcyI 3 GGCGTC G[GA]^CG[CT]C
535 GACGCC
AocI 364 CCTCAGG CC^T[ACGT]AGG
760 CCTGAGG
AvaII 392 GGACC G^G[AT]CC
903 GGACC
BsaHI 3 GGCGTC G[GA]^CG[CT]C
535 GACGCC
BsaWI 174 TCCGGT [AT]^CCGG[AT]
738 ACCGGA
BstF5I 885 GGATG GGATG(2/0)
975 GGATG
Bsu36I 364 CCTCAGG CC^T[ACGT]AGG
760 CCTGAGG
DpnII 802 GATC ^GATC
921 GATC
Eco81I 364 CCTCAGG CC^T[ACGT]AGG
760 CCTGAGG
EcoT14I 356 CCTTGG C^C[AT][AT]GG
644 CCAAGG
FokI 885 GGATG GGATG(9/13)
975 GGATG
HaeII 288 AGCGCC [GA]GCGC^[CT]
312 AGCGCC
Hin1I 3 GGCGTC G[GA]^CG[CT]C
535 GACGCC
MboI 802 GATC ^GATC
921 GATC
MslI 354 CACCTTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
383 CATCTGCGTG
MspA1I 148 CAGCTG C[AC]G^C[GT]G
725 CCGCGG
NdeII 802 GATC ^GATC
921 GATC
NlaIII 694 CATG CATG^
840 CATG
NlaIV 360 GGTGCC GG[ACGT]^[ACGT]CC
648 GGCCCC
Sau3AI 802 GATC ^GATC
921 GATC
SfaNI 769 GATGC GCATC(5/9)
790 GATGC
SfcI 1021 CTGCAG C^T[GA][CT]AG
1069 CTACAG
StyI 356 CCTTGG C^C[AT][AT]GG
644 CCAAGG
TfiI 115 GAATC G^A[AT]TC
966 GAATC
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# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AccII AciI AfaI AluI AspHI
BbvI BmyI BsaJI BseRI BsiHKAI
BsiYI BslI BsmFI Bsp1286I BsrI
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI EarI EcoRII Fnu4HI
HaeIII HapII HgaI HhaI HinP1I
HinfI HpaII ItaI Ksp632I MaeIII
MboII MnlI MseI MspI MvaI
MvnI MwoI PleI RsaI Sau96I
ScrFI Tru9I TseI Tsp509I