Restriction map for
pFN21AE1130
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# NO CUT ENZYMES ( 141 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AgeI AhdI Alw44I Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BanII BbrPI
BbvCI BcgI BfaI BfrI BglI
BglII BlnI BlpI BmyI Bpu1102I
BsaAI BsaBI BsaWI BseAI BseRI
BsgI BsiHKAI BsiWI BsmBI BsmI
Bsp1286I BspDI BspEI BspHI BspLU11I
BsrBI BssSI Bst1107I BstAPI BstBI
BstXI BstYI BstZ17I CelII ClaI
CpoI DraI DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRV EcoT22I FseI
FspI HindIII HinfI HpaI KpnI
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MunI
NaeI NcoI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SbfI SexAI SfiI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccI 496 GTCGAC GT^[AC][GT]AC
AcsI 358 GAATTC [GA]^AATT[CT]
AflIII 212 ACGTGT A^C[GA][CT]GT
AlwI 458 GGATC GGATC(4/5)
AlwNI 556 CAGGCGCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 336 CCTCAGG CC^T[ACGT]AGG
ApoI 358 GAATTC [GA]^AATT[CT]
AvaII 246 GGACC G^G[AT]CC
BanI 40 GGCGCC G^G[CT][GA]CC
BbeI 40 GGCGCC GGCGC^C
BbsI 601 GTCTTC GAAGAC(2/6)
BciVI 473 GTATCC GTATCC(6/5)
BclI 350 TGATCA T^GATCA
BpmI 85 CTGGAG CTGGAG(16/14)
BpuAI 601 GTCTTC GAAGAC(2/6)
BsaI 618 GGTCTC GGTCTC(1/5)
BsiEI 498 CGACCG CG[GA][CT]^CG
BsiYI 47 CCGGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 47 CCGGAGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 197 GGGAC GGGAC(10/14)
Bsp1407I 628 TGTACA T^GTACA
BspMI 101 ACCTGC ACCTGC(4/8)
BsrDI 448 CATTGC GCAATG(2/0)
BsrFI 500 ACCGGC [GA]^CCGG[CT]
BsrGI 628 TGTACA T^GTACA
Bsu36I 336 CCTCAGG CC^T[ACGT]AGG
Cfr10I 500 ACCGGC [GA]^CCGG[CT]
DdeI 337 CTCAG C^T[ACGT]AG
Eco81I 336 CCTCAGG CC^T[ACGT]AGG
EcoRI 358 GAATTC G^AATTC
EcoT14I 77 CCTTGG C^C[AT][AT]GG
FauI 509 CCCGC CCCGC(4/6)
FbaI 350 TGATCA T^GATCA
HgaI 256 GCGTC GACGC(5/10)
HincII 496 GTCGAC GT[CT]^[GA]AC
HindII 496 GTCGAC GT[CT]^[GA]AC
KasI 40 GGCGCC G^GCGCC
MaeII 212 ACGT A^CGT
MslI 576 CATTTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 142 CAGCTG C[AC]G^C[GT]G
NarI 40 GGCGCC GG^CGCC
NdeI 380 CATATG CA^TATG
NlaIII 177 CATG CATG^
PvuII 142 CAGCTG CAG^CTG
SalI 496 GTCGAC G^TCGAC
SapI 322 GAAGAGC GCTCTTC(1/4)
ScaI 485 AGTACT AGT^ACT
SfaNI 569 GCATC GCATC(5/9)
SfoI 40 GGCGCC GGC^GCC
SspBI 628 TGTACA T^GTACA
StyI 77 CCTTGG C^C[AT][AT]GG
TaqI 497 TCGA T^CGA
TspRI 67 CAGTG CA[GC]TG[ACGT][ACGT]^
TthHB8I 497 TCGA T^CGA
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# TWO CUT ENZYMES ( 26 enzymes) #
###################################
AcyI 40 GGCGCC G[GA]^CG[CT]C
255 GGCGTC
AluI 143 AGCT AG^CT
326 AGCT
AvaI 30 CTCGGG C^[CT]CG[GA]G
546 CCCGAG
BsaHI 40 GGCGCC G[GA]^CG[CT]C
255 GGCGTC
BsoBI 30 CTCGGG C^[CT]CG[GA]G
546 CCCGAG
BssHII 151 GCGCGC G^CGCGC
153 GCGCGC
BstEII 97 GGTCACC G^GT[ACGT]ACC
330 GGTGACC
BstF5I 117 CATCC GGATG(2/0)
570 CATCC
BstNI 93 CCTGG CC^[AT]GG
555 CCAGG
BstPI 97 GGTCACC G^GT[ACGT]ACC
330 GGTGACC
DpnII 351 GATC ^GATC
459 GATC
DraII 89 AGGCCCT [GA]G^G[ACGT]CC[CT]
183 GGGGCCT
EarI 322 GAAGAG CTCTTC(1/4)
427 GAAGAG
EcoO109I 89 AGGCCCT [GA]G^G[ACGT]CC[CT]
183 GGGGCCT
EcoO65I 97 GGTCACC G^GT[ACGT]ACC
330 GGTGACC
EcoRII 93 CCTGG ^CC[AT]GG
555 CCAGG
FokI 117 CATCC GGATG(9/13)
570 CATCC
HaeII 40 GGCGCC [GA]GCGC^[CT]
558 GGCGCT
Hin1I 40 GGCGCC G[GA]^CG[CT]C
255 GGCGTC
HphI 330 GGTGA GGTGA(8/7)
99 TCACC
Ksp632I 322 GAAGAG CTCTTC(1/4)
427 GAAGAG
MboI 351 GATC ^GATC
459 GATC
MvaI 93 CCTGG CC^[AT]GG
555 CCAGG
NdeII 351 GATC ^GATC
459 GATC
Sau3AI 351 GATC ^GATC
459 GATC
Tsp45I 98 GTCAC ^GT[GC]AC
331 GTGAC
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# 3 OR MORE CUT ENZYMES ( 37 enzymes) #
#########################################
AccII AciI AfaI BbvI BcnI
BsaJI BsmAI BsrI BssKI BstUI
Cac8I CfoI Cfr13I Fnu4HI HaeIII
HapII HhaI HinP1I HpaII ItaI
MaeIII MboII MnlI MseI MspI
MvnI MwoI NciI NlaIV PstI
RsaI Sau96I ScrFI SfcI Tru9I
TseI Tsp509I