Restriction map for
pFN21AE1162
##################################
# NO CUT ENZYMES ( 147 enzymes) #
##################################
AatII Acc65I AccI AccIII AciI
AclI AcyI AflII AgeI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspHI AvaII AviII AvrII
BalI BamHI BanI BanII BbeI
BciVI BclI BfrI BglI BlnI
BlpI BmyI Bpu1102I BsaBI BsaHI
BsaWI BseAI BseRI BsgI BsiEI
BsiHKAI BsiWI BsmBI Bsp1286I Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstXI BstZ17I Bsu36I Cac8I
CelII Cfr10I ClaI CpoI DraI
DraII DrdI EaeI EagI EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoRV FauI FbaI FseI FspI
HaeII Hin1I HpaI KasI KpnI
KspI MamI MfeI MluI MluNI
MroI MscI MspA1I MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NspI PacI PaeR7I PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SfaNI SfiI SfoI SgrAI
SmaI SmlI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AfaI 733 GTAC GT^AC
AhdI 729 GACTGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 729 GACTGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 952 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 152 CCCGAG C^[CT]CG[GA]G
BbrPI 1020 CACGTG CAC^GTG
BbvCI 143 CCTCAGC CCTCAGC(-5/-2)
BbvI 486 GCAGC GCAGC(8/12)
BcgI 967 CGAAATTCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 120 CCCGG CC^[GC]GG
BglII 171 AGATCT A^GATCT
BsaAI 1020 CACGTG [CT]AC^GT[GA]
BsmFI 616 GGGAC GGGAC(10/14)
BsoBI 152 CCCGAG C^[CT]CG[GA]G
BstAPI 884 GCAACTCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstBI 1392 TTCGAA TT^CGAA
BstEII 220 GGTAACC G^GT[ACGT]ACC
BstPI 220 GGTAACC G^GT[ACGT]ACC
BstYI 171 AGATCT [GA]^GATC[CT]
Cfr13I 372 GGCCC G^G[ACGT]CC
DraIII 241 CACACAGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 546 CCATGG C^C[GA][CT]GG
Eam1105I 729 GACTGTACGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO65I 220 GGTAACC G^GT[ACGT]ACC
EcoT14I 546 CCATGG C^C[AT][AT]GG
EcoT22I 663 ATGCAT ATGCA^T
Fnu4HI 486 GCAGC GC^[ACGT]GC
HaeIII 372 GGCC GG^CC
HapII 121 CCGG C^CGG
HgaI 816 GCGTC GACGC(5/10)
HindIII 459 AAGCTT A^AGCTT
HpaII 121 CCGG C^CGG
HphI 562 TCACC GGTGA(8/7)
ItaI 486 GCAGC GC^[ACGT]GC
MflI 171 AGATCT [GA]^GATC[CT]
MspI 121 CCGG C^CGG
NciI 120 CCCGG CC^[GC]GG
NcoI 546 CCATGG C^CATGG
NsiI 663 ATGCAT ATGCA^T
NspV 1392 TTCGAA TT^CGAA
PflFI 952 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 1320 GAGTC GAGTC(4/5)
PmaCI 1020 CACGTG CAC^GTG
PmlI 1020 CACGTG CAC^GTG
PstI 484 CTGCAG CTGCA^G
RsaI 733 GTAC GT^AC
Sau96I 372 GGCCC G^G[ACGT]CC
SexAI 60 ACCTGGT A^CC[AT]GGT
SfuI 1392 TTCGAA TT^CGAA
StyI 546 CCATGG C^C[AT][AT]GG
TseI 486 GCAGC G^C[AT]GC
Tth111I 952 GACAAGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 171 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 409 CGCG CG^CG
815 CGCG
AflIII 1019 ACACGT A^C[GA][CT]GT
1021 ACGTGT
BbsI 958 GTCTTC GAAGAC(2/6)
1210 GTCTTC
BfaI 202 CTAG C^TAG
1571 CTAG
BpuAI 958 GTCTTC GAAGAC(2/6)
1210 GTCTTC
BsaI 401 GAGACC GGTCTC(1/5)
64 GGTCTC
BsiYI 69 CCCTGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1671 CCATACTAAGG
BslI 69 CCCTGGGCTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1671 CCATACTAAGG
BsmI 1389 GCATTC GAATGC(1/-1)
1473 GCATTC
BstUI 409 CGCG CG^CG
815 CGCG
DpnII 172 GATC ^GATC
775 GATC
EcoRI 1163 GAATTC G^AATTC
1247 GAATTC
HincII 541 GTCAAC GT[CT]^[GA]AC
1056 GTCAAC
HindII 541 GTCAAC GT[CT]^[GA]AC
1056 GTCAAC
MaeI 202 CTAG C^TAG
1571 CTAG
MaeII 735 ACGT A^CGT
1021 ACGT
MboI 172 GATC ^GATC
775 GATC
MvnI 409 CGCG CG^CG
815 CGCG
NdeII 172 GATC ^GATC
775 GATC
Sau3AI 172 GATC ^GATC
775 GATC
SfcI 52 CTACAG C^T[GA][CT]AG
484 CTGCAG
TaqI 966 TCGA T^CGA
1393 TCGA
TthHB8I 966 TCGA T^CGA
1393 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 35 enzymes) #
#########################################
AcsI AluI ApoI BpmI BsaJI
BsmAI BsrDI BsrI BssKI BstF5I
BstNI CfoI DdeI EarI EcoRII
FokI HhaI HinP1I HinfI Ksp632I
MaeIII MboII MnlI MseI MslI
MvaI MwoI NlaIII NlaIV ScrFI
TfiI Tru9I Tsp45I Tsp509I TspRI