Restriction map for
pFN21AE1133
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# NO CUT ENZYMES ( 128 enzymes) #
##################################
AatII AccI AclI AcsI AflII
AflIII AgeI AhdI AlwNI Aor51HI
ApoI AscI AseI AsnI Asp700I
AspEI AspI AviII AvrII BamHI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BseRI BsgI BsiWI BsmAI BsmBI
BsmI Bsp1407I BspDI BspHI BspLU11I
BspMI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoNI
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KspI MaeI
MamI MfeI MluI MseI MunI
NaeI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SbfI ScaI
SexAI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tru9I Tsp509I Tth111I TthHB8I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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Acc65I 478 GGTACC G^GTACC
Alw44I 772 GTGCAC G^TGCAC
ApaLI 772 GTGCAC G^TGCAC
Asp718I 478 GGTACC G^GTACC
AvaI 484 CCCGGG C^[CT]CG[GA]G
BbeI 193 GGCGCC GGCGC^C
BbvCI 46 GCTGAGG CCTCAGC(-5/-2)
BglII 884 AGATCT A^GATCT
BsiEI 354 CGACCG CG[GA][CT]^CG
BsoBI 484 CCCGGG C^[CT]CG[GA]G
BsrBI 622 GAGCGG CCGCTC(-3/-3)
BsrFI 814 ACCGGC [GA]^CCGG[CT]
CfoI 194 GCGC GCG^C
Cfr10I 814 ACCGGC [GA]^CCGG[CT]
DraIII 823 CACGATGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 910 CTCTTC CTCTTC(1/4)
HaeII 193 GGCGCC [GA]GCGC^[CT]
HhaI 194 GCGC GCG^C
HinP1I 194 GCGC G^CGC
HincII 472 GTCAAC GT[CT]^[GA]AC
HindII 472 GTCAAC GT[CT]^[GA]AC
HinfI 648 GATTC G^A[ACGT]TC
KasI 193 GGCGCC G^GCGCC
KpnI 478 GGTACC GGTAC^C
Ksp632I 910 CTCTTC CTCTTC(1/4)
MaeII 106 ACGT A^CGT
MslI 670 CACTACAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 193 GGCGCC GG^CGCC
PvuII 558 CAGCTG CAG^CTG
SapI 909 GCTCTTC GCTCTTC(1/4)
SfoI 193 GGCGCC GGC^GCC
SmaI 484 CCCGGG CCC^GGG
SmlI 856 CTCAAG C^T[CT][GA]AG
StuI 540 AGGCCT AGG^CCT
TfiI 648 GATTC G^A[AT]TC
XcmI 746 CCAACAAGCACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 484 CCCGGG C^CCGGG
XmaI 484 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccII 63 CGCG CG^CG
120 CGCG
AcyI 95 GACGCC G[GA]^CG[CT]C
193 GGCGCC
AfaI 479 GTAC GT^AC
786 GTAC
AlwI 138 GATCC GGATC(4/5)
713 GGATC
AocI 293 CCTCAGG CC^T[ACGT]AGG
833 CCTCAGG
AspHI 148 GTGCTC G[AT]GC[AT]^C
772 GTGCAC
BanI 193 GGCGCC G^G[CT][GA]CC
478 GGTACC
BsaHI 95 GACGCC G[GA]^CG[CT]C
193 GGCGCC
BsiHKAI 148 GTGCTC G[AT]GC[AT]^C
772 GTGCAC
BstEII 126 GGTAACC G^GT[ACGT]ACC
397 GGTAACC
BstPI 126 GGTAACC G^GT[ACGT]ACC
397 GGTAACC
BstUI 63 CGCG CG^CG
120 CGCG
BstXI 761 CCATCGGCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
842 CCACCATCCTGG
BstYI 713 GGATCT [GA]^GATC[CT]
884 AGATCT
Bsu36I 293 CCTCAGG CC^T[ACGT]AGG
833 CCTCAGG
DsaI 456 CCGTGG C^C[GA][CT]GG
616 CCACGG
Eco81I 293 CCTCAGG CC^T[ACGT]AGG
833 CCTCAGG
EcoO65I 126 GGTAACC G^GT[ACGT]ACC
397 GGTAACC
EcoT14I 464 CCAAGG C^C[AT][AT]GG
518 CCAAGG
Hin1I 95 GACGCC G[GA]^CG[CT]C
193 GGCGCC
HphI 865 GGTGA GGTGA(8/7)
725 TCACC
MboII 887 TCTTC GAAGA(8/7)
911 TCTTC
MflI 713 GGATCT [GA]^GATC[CT]
884 AGATCT
MvnI 63 CGCG CG^CG
120 CGCG
NlaIII 135 CATG CATG^
493 CATG
PflMI 184 CCACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
822 CCACGATGTGG
RsaI 479 GTAC GT^AC
786 GTAC
SfaNI 805 GATGC GCATC(5/9)
791 GCATC
StyI 464 CCAAGG C^C[AT][AT]GG
518 CCAAGG
Tsp45I 601 GTGAC ^GT[GC]AC
866 GTGAC
Van91I 184 CCACTGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
822 CCACGATGTGG
XhoII 713 GGATCT [GA]^GATC[CT]
884 AGATCT
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# 3 OR MORE CUT ENZYMES ( 60 enzymes) #
#########################################
AccIII AciI AluI ApaI AvaII
BalI BanII BbvI BcnI BglI
BmyI BsaJI BsaWI BseAI BsiYI
BslI BsmFI Bsp1286I BspEI BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HpaII ItaI MaeIII
MboI MluNI MnlI MroI MscI
MspA1I MspI MvaI MwoI NciI
NdeII NlaIV PstI Sau3AI Sau96I
ScrFI SfcI SfiI TseI TspRI