Restriction map for
pFN21AB4905
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# NO CUT ENZYMES ( 140 enzymes) #
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AatII AccI AccIII AclI AcsI
AflII AgeI AhdI Alw44I AlwI
AlwNI AocI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BbeI BbsI BcgI BclI
BfrI BglII BlnI BpuAI BsaBI
BsaI BsaWI BseAI BseRI BsgI
BsiWI BsmAI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrDI BsrGI Bst1107I BstAPI
BstBI BstEII BstPI BstYI BstZ17I
Bsu36I ClaI CpoI DpnII DraI
DraII DrdI Eam1105I EarI Eco81I
EcoO109I EcoO65I EcoRI EcoRV EcoT22I
FbaI FspI HincII HindII HindIII
HpaI KasI Ksp632I MamI MboI
MfeI MflI MroI MunI NarI
NcoI NdeI NdeII NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PflMI PinAI PmeI PpuMI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SalI SapI
Sau3AI SbfI ScaI SexAI SfoI
SfuI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tsp509I Tth111I Van91I XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 35 enzymes) #
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Acc65I 512 GGTACC G^GTACC
AflIII 854 ACGCGT A^C[GA][CT]GT
Aor51HI 146 AGCGCT AGC^GCT
Asp718I 512 GGTACC G^GTACC
BbrPI 712 CACGTG CAC^GTG
BbvCI 727 GCTGAGG CCTCAGC(-5/-2)
BciVI 125 GTATCC GTATCC(6/5)
BlpI 786 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 786 GCTGAGC GC^T[ACGT]AGC
BsaAI 712 CACGTG [CT]AC^GT[GA]
BsiEI 657 CGGCCG CG[GA][CT]^CG
BsmFI 372 GTCCC GGGAC(10/14)
BssHII 740 GCGCGC G^CGCGC
BssSI 772 CTCGTG CACGAG(-5/-1)
CelII 786 GCTGAGC GC^T[ACGT]AGC
EagI 657 CGGCCG C^GGCCG
EclXI 657 CGGCCG C^GGCCG
Eco47III 146 AGCGCT AGC^GCT
Eco52I 657 CGGCCG C^GGCCG
EcoNI 723 CCTGGCTGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
FseI 747 GGCCGGCC GGCCGG^CC
HphI 717 GGTGA GGTGA(8/7)
KpnI 512 GGTACC GGTAC^C
MluI 854 ACGCGT A^CGCGT
MseI 1042 TTAA T^TAA
NspI 299 GCATGT [GA]CATG^[CT]
PmaCI 712 CACGTG CAC^GTG
PmlI 712 CACGTG CAC^GTG
PstI 17 CTGCAG CTGCA^G
SgrAI 861 CGCCGGCG C[GA]^CCGG[CT]G
SmlI 1036 CTCAAG C^T[CT][GA]AG
TaqI 551 TCGA T^CGA
Tru9I 1042 TTAA T^TAA
TthHB8I 551 TCGA T^CGA
XbaI 336 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AcyI 614 GGCGTC G[GA]^CG[CT]C
933 GGCGTC
AspHI 670 GTGCTC G[AT]GC[AT]^C
805 GTGCTC
BalI 443 TGGCCA TGG^CCA
593 TGGCCA
BsaHI 614 GGCGTC G[GA]^CG[CT]C
933 GGCGTC
BsiHKAI 670 GTGCTC G[AT]GC[AT]^C
805 GTGCTC
BsrI 258 ACTGG ACTGG(1/-1)
348 ACTGG
BstXI 389 CCAAGGCGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
905 CCAAGCTGCTGG
DraIII 32 CACTAGGTG CAC[ACGT][ACGT][ACGT]^GTG
712 CACGTGGTG
DsaI 508 CCGCGG C^C[GA][CT]GG
948 CCGCGG
EcoT14I 237 CCAAGG C^C[AT][AT]GG
389 CCAAGG
Hin1I 614 GGCGTC G[GA]^CG[CT]C
933 GGCGTC
HinfI 293 GAGTC G^A[ACGT]TC
333 GAGTC
KspI 508 CCGCGG CCGC^GG
948 CCGCGG
MaeII 713 ACGT A^CGT
803 ACGT
MboII 13 TCTTC GAAGA(8/7)
548 TCTTC
MluNI 443 TGGCCA TGG^CCA
593 TGGCCA
MscI 443 TGGCCA TGG^CCA
593 TGGCCA
MspA1I 508 CCGCGG C[AC]G^C[GT]G
948 CCGCGG
NaeI 748 GCCGGC GCC^GGC
862 GCCGGC
NgoMIV 748 GCCGGC G^CCGGC
862 GCCGGC
PleI 293 GAGTC GAGTC(4/5)
333 GAGTC
SacII 508 CCGCGG CCGC^GG
948 CCGCGG
SfaNI 138 GATGC GCATC(5/9)
419 GCATC
SfcI 17 CTGCAG C^T[GA][CT]AG
622 CTACAG
SfiI 186 GGCCGACCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
747 GGCCGGCCAGGCC
StyI 237 CCAAGG C^C[AT][AT]GG
389 CCAAGG
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# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AccII AciI AfaI AluI BanI
BanII BbvI BcnI BfaI BglI
BmyI BpmI BsaJI BsiYI BslI
Bsp1286I BsrBI BsrFI BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr10I
Cfr13I DdeI EaeI EcoRII FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI HinP1I HpaII ItaI
MaeI MaeIII MnlI MslI MspI
MvaI MvnI MwoI NciI NlaIII
NlaIV RsaI Sau96I ScrFI TseI
Tsp45I TspRI