Restriction map for
pFN21AB5305
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# NO CUT ENZYMES ( 126 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AspHI
AspI AvaI AviII AvrII BamHI
BbeI BbrPI BbvCI BciVI BclI
BfrI BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaI BseAI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspLU11I BsrBI BsrGI
BssHII BssSI Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
Hin1I HpaI KasI KpnI KspI
MamI MluI MroI NaeI NarI
NgoMIV NheI NotI NspI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI StuI SwaI
Tth111I XbaI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AccI 1321 GTCTAC GT^[AC][GT]AC
AcsI 1236 AAATTT [GA]^AATT[CT]
AfaI 419 GTAC GT^AC
ApoI 1236 AAATTT [GA]^AATT[CT]
AseI 190 ATTAAT AT^TAAT
AsnI 190 ATTAAT AT^TAAT
BanII 281 GAGCCC G[GA]GC[CT]^C
BbsI 1452 GAAGAC GAAGAC(2/6)
BcgI 51 CGACTTAGTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 284 CCCGG CC^[GC]GG
BfaI 1489 CTAG C^TAG
BglI 1466 GCCACGAAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1421 AGATCT A^GATCT
BpmI 203 CTGGAG CTGGAG(16/14)
BpuAI 1452 GAAGAC GAAGAC(2/6)
BseRI 1063 GAGGAG GAGGAG(10/8)
BsgI 637 CTGCAC GTGCAG(16/14)
BsiEI 65 CGGTCG CG[GA][CT]^CG
BspHI 498 TCATGA T^CATGA
BsrFI 1267 ACCGGC [GA]^CCGG[CT]
Cfr10I 1267 ACCGGC [GA]^CCGG[CT]
DrdI 310 GACTCAGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 1071 CCTGCATCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1154 ATGCAT ATGCA^T
HaeII 1037 GGCGCT [GA]GCGC^[CT]
HincII 877 GTCAAC GT[CT]^[GA]AC
HindII 877 GTCAAC GT[CT]^[GA]AC
HphI 1276 GGTGA GGTGA(8/7)
MaeI 1489 CTAG C^TAG
MfeI 830 CAATTG C^AATTG
MunI 830 CAATTG C^AATTG
NciI 284 CCCGG CC^[GC]GG
NcoI 1377 CCATGG C^CATGG
NdeI 1529 CATATG CA^TATG
NruI 10 TCGCGA TCG^CGA
NsiI 1154 ATGCAT ATGCA^T
PshAI 1353 GACTGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 190 ATTAAT AT^TAAT
RcaI 498 TCATGA T^CATGA
RsaI 419 GTAC GT^AC
SapI 223 GAAGAGC GCTCTTC(1/4)
SexAI 490 ACCTGGT A^CC[AT]GGT
SmlI 1058 CTCAAG C^T[CT][GA]AG
SspI 187 AATATT AAT^ATT
TfiI 600 GATTC G^A[AT]TC
XcmI 851 CCACTGTCTCCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 27 enzymes) #
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AccII 11 CGCG CG^CG
977 CGCG
AlwI 1068 GATCC GGATC(4/5)
173 GGATC
BanI 1012 GGCACC G^G[CT][GA]CC
1399 GGTGCC
BbvI 1509 GCAGC GCAGC(8/12)
636 GCTGC
BmyI 281 GAGCCC G[AGT]GC[ACT]^C
1011 GGGCAC
BsaWI 63 TCCGGT [AT]^CCGG[AT]
650 TCCGGT
Bsp1286I 281 GAGCCC G[AGT]GC[ACT]^C
1011 GGGCAC
BspMI 1018 ACCTGC ACCTGC(4/8)
1285 ACCTGC
BstAPI 630 GCAAGAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1013 GCACCACCTGC
BstUI 11 CGCG CG^CG
977 CGCG
BstXI 725 CCAAGATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1116 CCATCAAGATGG
BstYI 1067 AGATCC [GA]^GATC[CT]
1421 AGATCT
CfoI 976 GCGC GCG^C
1038 GCGC
DsaI 23 CCACGG C^C[GA][CT]GG
1377 CCATGG
FauI 1446 CCCGC CCCGC(4/6)
978 GCGGG
HhaI 976 GCGC GCG^C
1038 GCGC
HinP1I 976 GCGC G^CGC
1038 GCGC
HindIII 323 AAGCTT A^AGCTT
913 AAGCTT
MaeII 1002 ACGT A^CGT
1188 ACGT
MflI 1067 AGATCC [GA]^GATC[CT]
1421 AGATCT
MslI 1092 CATGGGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1117 CATCAAGATG
MspA1I 1309 CAGCGG C[AC]G^C[GT]G
1447 CCGCTG
MvnI 11 CGCG CG^CG
977 CGCG
PflMI 1086 CCATAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1418 CCAAGATCTGG
TseI 636 GCTGC G^C[AT]GC
1509 GCAGC
Van91I 1086 CCATAGCATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1418 CCAAGATCTGG
XhoII 1067 AGATCC [GA]^GATC[CT]
1421 AGATCT
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# 3 OR MORE CUT ENZYMES ( 59 enzymes) #
#########################################
AciI AluI AvaII BalI BsaJI
BsiYI BslI BsmAI BsmFI BsrDI
BsrI BssKI BstF5I BstNI Cac8I
Cfr13I DdeI DpnII DraII EaeI
EarI EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HgaI HinfI
HpaII ItaI Ksp632I MaeIII MboI
MboII MluNI MnlI MscI MseI
MspI MvaI MwoI NdeII NlaIII
NlaIV PleI PpuMI Sau3AI Sau96I
ScrFI SfaNI StyI TaqI Tru9I
Tsp45I Tsp509I TspRI TthHB8I