##################################
# NO CUT ENZYMES (  212 enzymes) #
##################################

AatII       Acc65I      AccII       AccIII      AciI        
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwNI       AocI        Aor51HI     
ApaI        ApaLI       AscI        AseI        AsnI        
Asp718I     AspEI       AspHI       AspI        AvaI        
AviII       AvrII       BalI        BamHI       BanI        
BanII       BbeI        BbrPI       BbsI        BbvCI       
BbvI        BcgI        BciVI       BclI        BcnI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmBI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BsrBI       BsrFI       BsrGI       BssHII      
BssKI       BssSI       Bst1107I    BstAPI      BstEII      
BstF5I      BstNI       BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
CfoI        Cfr10I      ClaI        CpoI        DraI        
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRII      EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        Fnu4HI      FokI        FseI        FspI        
HaeII       HaeIII      HapII       HgaI        HhaI        
Hin1I       HinP1I      HincII      HindII      HindIII     
HpaI        HpaII       ItaI        KasI        KpnI        
Ksp632I     KspI        MaeII       MaeIII      MamI        
MfeI        MflI        MluI        MluNI       MroI        
MscI        MslI        MspA1I      MspI        MunI        
MvaI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NgoMIV      NheI        
NotI        NruI        NsiI        NspI        PacI        
PaeR7I      PflFI       PflMI       PinAI       PmaCI       
PmeI        PmlI        PpuMI       PshAI       PshBI       
Psp1406I    PstI        PvuI        PvuII       RcaI        
RsrII       SacI        SacII       SalI        SapI        
SbfI        ScaI        ScrFI       SexAI       SfiI        
SfoI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        TseI        Tsp45I      
Tth111I     Van91I      XcmI        XhoI        XhoII       
XmaCI       XmaI        


###################################
# ONE CUT ENZYMES (   25 enzymes) #
###################################

AccI          373  GTAGAC               GT^[AC][GT]AC
AlwI           97  GATCC                GGATC(4/5)
Asp700I       271  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BseRI         184  GAGGAG               GAGGAG(10/8)
BsmAI         152  GTCTC                GTCTC(1/5)
BsmFI         203  GGGAC                GGGAC(10/14)
BspMI         175  GCAGGT               ACCTGC(4/8)
BsrDI         196  GCAATG               GCAATG(2/0)
BstBI         488  TTCGAA               TT^CGAA
DpnII          97  GATC                 ^GATC
EcoRI         413  GAATTC               G^AATTC
HphI          279  TCACC                GGTGA(8/7)
MboI           97  GATC                 ^GATC
NdeII          97  GATC                 ^GATC
NlaIII        213  CATG                 CATG^
NlaIV         203  GGGACC               GG[ACGT]^[ACGT]CC
NspV          488  TTCGAA               TT^CGAA
PleI          313  GACTC                GAGTC(4/5)
Sau3AI         97  GATC                 ^GATC
SfaNI         460  GATGC                GCATC(5/9)
SfcI          117  CTACAG               C^T[GA][CT]AG
SfuI          488  TTCGAA               TT^CGAA
TspRI         192  CAGTG                CA[GC]TG[ACGT][ACGT]^
XbaI          364  TCTAGA               T^CTAGA
XmnI          271  GAAGATATTC           GAA[ACGT][ACGT]^[ACGT][ACGT]TTC


###################################
# TWO CUT ENZYMES (   13 enzymes) #
###################################

AcsI          413  GAATTC               [GA]^AATT[CT]
              485  AAATTC              
AluI           30  AGCT                 AG^CT
               65  AGCT                
ApoI          413  GAATTC               [GA]^AATT[CT]
              485  AAATTC              
AvaII         204  GGACC                G^G[AT]CC
              333  GGTCC               
BfaI          337  CTAG                 C^TAG
              365  CTAG                
BsrI          220  ACTGG                ACTGG(1/-1)
              207  CCAGT               
Cfr13I        204  GGACC                G^G[ACGT]CC
              333  GGTCC               
DdeI          389  CTCAG                C^T[ACGT]AG
              396  CTTAG               
MaeI          337  CTAG                 C^TAG
              365  CTAG                
MseI          299  TTAA                 T^TAA
              308  TTAA                
Sau96I        204  GGACC                G^G[ACGT]CC
              333  GGTCC               
TfiI           75  GAATC                G^A[AT]TC
              112  GATTC               
Tru9I         299  TTAA                 T^TAA
              308  TTAA                


#########################################
# 3 OR MORE CUT ENZYMES (    8 enzymes) #
#########################################

AfaI        HinfI       MboII       MnlI        RsaI        
TaqI        Tsp509I     TthHB8I