Restriction map for
pFN21AB9955
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# NO CUT ENZYMES ( 110 enzymes) #
##################################
AatII AccI AccIII AclI AcyI
AflII AgeI Alw44I AlwNI AocI
Aor51HI ApaLI AscI AseI AsnI
AviII AvrII BalI BamHI BbeI
BbrPI BcgI BfrI BlnI BsaAI
BsaBI BsaHI BsaWI BseAI BsgI
BsiEI BsmI BspDI BspEI BspLU11I
BsrFI BssHII BssSI Bst1107I BstAPI
BstBI BstZ17I Bsu36I Cfr10I ClaI
CpoI DraI DraIII DsaI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI Hin1I HindIII HpaI
KasI KspI MamI MfeI MluI
MluNI MroI MscI MspA1I MunI
NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RsrII SacII SalI SbfI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I StuI SwaI XbaI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AflIII 1343 ACGTGT A^C[GA][CT]GT
AlwI 1497 GATCC GGATC(4/5)
ApaI 653 GGGCCC GGGCC^C
AspI 592 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BbvCI 106 CCTCAGC CCTCAGC(-5/-2)
BciVI 634 GGATAC GTATCC(6/5)
BclI 818 TGATCA T^GATCA
BglI 1300 GCCCCCGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1167 AGATCT A^GATCT
BlpI 475 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 475 GCTGAGC GC^T[ACGT]AGC
BsiWI 229 CGTACG C^GTACG
BsmBI 209 CGTCTC CGTCTC(1/5)
Bsp1407I 681 TGTACA T^GTACA
BsrBI 366 CCGCTC CCGCTC(-3/-3)
BsrDI 12 GCAATG GCAATG(2/0)
BsrGI 681 TGTACA T^GTACA
BstEII 1377 GGTCACC G^GT[ACGT]ACC
BstPI 1377 GGTCACC G^GT[ACGT]ACC
BstXI 69 CCAGAGCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1167 AGATCT [GA]^GATC[CT]
CelII 475 GCTGAGC GC^T[ACGT]AGC
DrdI 970 GACAAGCTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 462 TGGCCG [CT]^GGCC[GA]
EcoO65I 1377 GGTCACC G^GT[ACGT]ACC
FbaI 818 TGATCA T^GATCA
HincII 421 GTTGAC GT[CT]^[GA]AC
HindII 421 GTTGAC GT[CT]^[GA]AC
MaeII 1343 ACGT A^CGT
MflI 1167 AGATCT [GA]^GATC[CT]
MslI 1185 CATTCTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NdeI 1206 CATATG CA^TATG
PaeR7I 800 CTCGAG C^TCGAG
PflFI 592 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 1286 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SapI 1076 GAAGAGC GCTCTTC(1/4)
ScaI 1541 AGTACT AGT^ACT
SexAI 1596 ACCTGGT A^CC[AT]GGT
SmaI 650 CCCGGG CCC^GGG
SspBI 681 TGTACA T^GTACA
SspI 330 AATATT AAT^ATT
Tth111I 592 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 1286 CCATCAAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 800 CTCGAG C^TCGAG
XhoII 1167 AGATCT [GA]^GATC[CT]
XmaCI 650 CCCGGG C^CCGGG
XmaI 650 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 35 enzymes) #
###################################
AccII 526 CGCG CG^CG
1575 CGCG
AcsI 322 AAATTT [GA]^AATT[CT]
611 AAATTC
AhdI 179 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1342 GACGTGTGGTC
ApoI 322 AAATTT [GA]^AATT[CT]
611 AAATTC
Asp700I 319 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
664 GAAACACTTC
AspEI 179 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1342 GACGTGTGGTC
BbsI 1246 GAAGAC GAAGAC(2/6)
1561 GAAGAC
BfaI 724 CTAG C^TAG
1189 CTAG
BpuAI 1246 GAAGAC GAAGAC(2/6)
1561 GAAGAC
BsaI 590 GAGACC GGTCTC(1/5)
709 GGTCTC
BspHI 950 TCATGA T^CATGA
1493 TCATGA
BspMI 706 GCAGGT ACCTGC(4/8)
1431 GCAGGT
BstUI 526 CGCG CG^CG
1575 CGCG
Cac8I 364 GCCCGC GC[ACGT]^[ACGT]GC
1460 GCCCGC
CfoI 943 GCGC GCG^C
1146 GCGC
DraII 220 GGGACCT [GA]G^G[ACGT]CC[CT]
1514 AGGACCC
Eam1105I 179 GACTCACCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
1342 GACGTGTGGTC
EcoO109I 220 GGGACCT [GA]G^G[ACGT]CC[CT]
1514 AGGACCC
EcoT14I 174 CCAAGG C^C[AT][AT]GG
751 CCAAGG
FauI 365 CCCGC CCCGC(4/6)
1461 CCCGC
HhaI 943 GCGC GCG^C
1146 GCGC
HinP1I 943 GCGC G^CGC
1146 GCGC
MaeI 724 CTAG C^TAG
1189 CTAG
MseI 902 TTAA T^TAA
1060 TTAA
MvnI 526 CGCG CG^CG
1575 CGCG
PpuMI 220 GGGACCT [GA]G^G[AT]CC[CT]
1514 AGGACCC
RcaI 950 TCATGA T^CATGA
1493 TCATGA
SmlI 800 CTCGAG C^T[CT][GA]AG
934 CTTGAG
StyI 174 CCAAGG C^C[AT][AT]GG
751 CCAAGG
TaqI 801 TCGA T^CGA
1142 TCGA
Tru9I 902 TTAA T^TAA
1060 TTAA
TspRI 992 CAGTG CA[GC]TG[ACGT][ACGT]^
1501 CACTG
TthHB8I 801 TCGA T^CGA
1142 TCGA
XcmI 401 CCAGCAATTATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1587 CCAGTACCAACCTGG
XmnI 319 GAAAAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
664 GAAACACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
Acc65I AciI AfaI AluI Asp718I
AspHI AvaI AvaII BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BseRI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BsrI BssKI
BstF5I BstNI Cfr13I DdeI DpnII
EarI EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
KpnI Ksp632I MaeIII MboI MboII
MnlI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV PleI PstI
RsaI SacI Sau3AI Sau96I ScrFI
SfaNI SfcI TfiI TseI Tsp45I
Tsp509I