Restriction map for
pFN21AB9292
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# NO CUT ENZYMES ( 137 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AlwI
AlwNI AocI ApoI AscI AseI
AsnI Asp700I Asp718I AspI AvaII
AviII BalI BamHI BbrPI BbvCI
BcgI BciVI BclI BfrI BglII
BlpI Bpu1102I BsaAI BsaBI BsaI
BsaWI BseAI BsiWI BsmBI BsmI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrDI BsrGI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I CelII ClaI CpoI DraI
DrdI Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HindIII HpaI KpnI
KspI MaeIII MamI MfeI MflI
MluI MluNI MroI MscI MseI
MspA1I MunI NdeI NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RsrII
SacII SalI SbfI SexAI SfcI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tru9I Tsp45I Tsp509I
Tth111I Van91I XbaI XcmI XhoI
XhoII XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AhdI 178 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 628 GTGCAC G^TGCAC
Aor51HI 38 AGCGCT AGC^GCT
ApaLI 628 GTGCAC G^TGCAC
AspEI 178 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 6 CCCGGG C^[CT]CG[GA]G
AvrII 102 CCTAGG C^CTAGG
BanI 202 GGCGCC G^G[CT][GA]CC
BbeI 202 GGCGCC GGCGC^C
BglI 158 GCCCGGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 102 CCTAGG C^CTAGG
BpmI 118 CTGGAG CTGGAG(16/14)
BsgI 91 CTGCAC GTGCAG(16/14)
BsiEI 161 CGGCCG CG[GA][CT]^CG
BsmAI 421 GAGAC GTCTC(1/5)
BsoBI 6 CCCGGG C^[CT]CG[GA]G
BspHI 639 TCATGA T^CATGA
BssHII 289 GCGCGC G^CGCGC
BssSI 631 CACGAG CACGAG(-5/-1)
DpnII 515 GATC ^GATC
DraIII 715 CACTCTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 769 CCATGG C^C[GA][CT]GG
EaeI 161 CGGCCG [CT]^GGCC[GA]
EagI 161 CGGCCG C^GGCCG
Eam1105I 178 GACTCGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 21 GAAGAG CTCTTC(1/4)
EclXI 161 CGGCCG C^GGCCG
Eco47III 38 AGCGCT AGC^GCT
Eco52I 161 CGGCCG C^GGCCG
KasI 202 GGCGCC G^GCGCC
Ksp632I 21 GAAGAG CTCTTC(1/4)
MaeII 608 ACGT A^CGT
MboI 515 GATC ^GATC
MslI 218 CACCCCGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 202 GGCGCC GG^CGCC
NcoI 769 CCATGG C^CATGG
NdeII 515 GATC ^GATC
RcaI 639 TCATGA T^CATGA
SacI 710 GAGCTC GAGCT^C
SapI 21 GAAGAGC GCTCTTC(1/4)
Sau3AI 515 GATC ^GATC
ScaI 442 AGTACT AGT^ACT
SfaNI 543 GCATC GCATC(5/9)
SfoI 202 GGCGCC GGC^GCC
SmaI 6 CCCGGG CCC^GGG
XmaCI 6 CCCGGG C^CCGGG
XmaI 6 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AcyI 183 GGCGTC G[GA]^CG[CT]C
202 GGCGCC
AspHI 628 GTGCAC G[AT]GC[AT]^C
710 GAGCTC
BbsI 597 GAAGAC GAAGAC(2/6)
668 GAAGAC
BfaI 103 CTAG C^TAG
479 CTAG
BpuAI 597 GAAGAC GAAGAC(2/6)
668 GAAGAC
BsaHI 183 GGCGTC G[GA]^CG[CT]C
202 GGCGCC
BsiHKAI 628 GTGCAC G[AT]GC[AT]^C
710 GAGCTC
BsrBI 620 CCGCTC CCGCTC(-3/-3)
12 GAGCGG
DdeI 446 CTGAG C^T[ACGT]AG
759 CTCAG
EcoT14I 102 CCTAGG C^C[AT][AT]GG
769 CCATGG
HaeII 38 AGCGCT [GA]GCGC^[CT]
202 GGCGCC
Hin1I 183 GGCGTC G[GA]^CG[CT]C
202 GGCGCC
HphI 18 GGTGA GGTGA(8/7)
433 TCACC
MaeI 103 CTAG C^TAG
479 CTAG
StyI 102 CCTAGG C^C[AT][AT]GG
769 CCATGG
TaqI 453 TCGA T^CGA
520 TCGA
TfiI 636 GATTC G^A[AT]TC
643 GAATC
TspRI 368 CACTG CA[GC]TG[ACGT][ACGT]^
374 CAGTG
TthHB8I 453 TCGA T^CGA
520 TCGA
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AccII AciI AfaI AluI ApaI
BanII BbvI BcnI BmyI BsaJI
BseRI BsiYI BslI BsmFI Bsp1286I
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DraII EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII ItaI MboII
MnlI MspI MvaI MvnI MwoI
NaeI NciI NgoMIV NlaIII NlaIV
PleI RsaI Sau96I ScrFI TseI