##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AflII       AflIII      AgeI        
AhdI        Alw44I      AlwI        AlwNI       AocI        
Aor51HI     ApaI        ApaLI       AscI        AseI        
AsnI        Asp700I     Asp718I     AspEI       AspHI       
AspI        AvaI        AvaII       AvrII       BamHI       
BanI        BanII       BbeI        BbrPI       BbsI        
BbvCI       BcgI        BclI        BcnI        BfaI        
BfrI        BglI        BglII       BlnI        BlpI        
BmyI        BpmI        Bpu1102I    BpuAI       BsaAI       
BsaBI       BsaHI       BsaI        BsaJI       BsaWI       
BseAI       BsgI        BsiEI       BsiHKAI     BsiWI       
BsiYI       BslI        BsmFI       BsmI        BsoBI       
Bsp1286I    Bsp1407I    BspDI       BspEI       BspHI       
BspLU11I    BspMI       BsrBI       BsrDI       BsrFI       
BsrGI       BsrI        BssHII      BssSI       Bst1107I    
BstAPI      BstBI       BstEII      BstPI       BstUI       
BstXI       BstYI       BstZ17I     Bsu36I      CelII       
Cfr10I      Cfr13I      ClaI        CpoI        DpnII       
DraI        DraII       DraIII      DrdI        DsaI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FseI        HaeII       HapII       Hin1I       
HincII      HindII      HindIII     HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MfeI        MflI        
MluI        MroI        MseI        MslI        MspI        
MunI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshBI       Psp1406I    PstI        
PvuI        RcaI        RsrII       SacI        SacII       
SalI        SapI        Sau3AI      Sau96I      SbfI        
ScaI        SexAI       SfaNI       SfcI        SfiI        
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SpeI        SphI        Sse8387I    SspBI       
SspI        StuI        StyI        SwaI        TaqI        
Tru9I       Tth111I     TthHB8I     Van91I      XbaI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   42 enzymes) #
###################################

AciI           62  GCGG                 CCGC(-3/-1)
AcsI          127  AAATTT               [GA]^AATT[CT]
AfaI            8  GTAC                 GT^AC
AluI          152  AGCT                 AG^CT
ApoI          127  AAATTT               [GA]^AATT[CT]
AviII         217  TGCGCA               TGC^GCA
BalI          155  TGGCCA               TGG^CCA
BciVI         100  GGATAC               GTATCC(6/5)
BseRI         259  CTCCTC               GAGGAG(10/8)
BsmAI         257  GTCTC                GTCTC(1/5)
BsmBI         256  CGTCTC               CGTCTC(1/5)
BssKI          24  CCTGG                ^CC[ACGT]GG
BstF5I        121  CATCC                GGATG(2/0)
BstNI          24  CCTGG                CC^[AT]GG
CfoI          218  GCGC                 GCG^C
DdeI          262  CTCAG                C^T[ACGT]AG
EaeI          155  TGGCCA               [CT]^GGCC[GA]
EcoRII         24  CCTGG                ^CC[AT]GG
FokI          121  CATCC                GGATG(9/13)
FspI          217  TGCGCA               TGC^GCA
HaeIII        156  GGCC                 GG^CC
HgaI          255  GCGTC                GACGC(5/10)
HhaI          218  GCGC                 GCG^C
HinP1I        218  GCGC                 G^CGC
HinfI          67  GAATC                G^A[ACGT]TC
HphI          240  GGTGA                GGTGA(8/7)
MaeIII        170  GTGAC                ^GT[ACGT]AC
MboII         117  TCTTC                GAAGA(8/7)
MluNI         155  TGGCCA               TGG^CCA
MscI          155  TGGCCA               TGG^CCA
MspA1I        151  CAGCTG               C[AC]G^C[GT]G
MvaI           24  CCTGG                CC^[AT]GG
NlaIII        282  CATG                 CATG^
PshAI          88  GACTGCTGTC           GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII         151  CAGCTG               CAG^CTG
RsaI            8  GTAC                 GT^AC
ScrFI          24  CCTGG                CC^[ACGT]GG
TfiI           67  GAATC                G^A[AT]TC
Tsp45I        170  GTGAC                ^GT[GC]AC
Tsp509I       128  AATT                 ^AATT
TspRI          41  CAGTG                CA[GC]TG[ACGT][ACGT]^
XcmI          226  CCAAAACAGACTTGG      CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG


###################################
# TWO CUT ENZYMES (    2 enzymes) #
###################################

Cac8I          76  GCAAGC               GC[ACGT]^[ACGT]GC
              153  GCTGGC              
MnlI           57  CCTC                 CCTC(7/6)
              261  CCTC                


#########################################
# 3 OR MORE CUT ENZYMES (    4 enzymes) #
#########################################

BbvI        Fnu4HI      ItaI        TseI