Restriction map for
pFN21AE3612
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# NO CUT ENZYMES ( 169 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AviII
AvrII BalI BamHI BbrPI BbsI
BcgI BciVI BclI BfaI BfrI
BglII BlnI BlpI BpmI Bpu1102I
BpuAI BsaAI BsaBI BsaI BsaWI
BseAI BsgI BsiEI BsiHKAI BsiWI
BsmAI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DraIII DrdI EagI Eam1105I EarI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO109I EcoO65I EcoRI EcoRV EcoT14I
EcoT22I FbaI FseI FspI HincII
HindII HindIII HpaI HphI KpnI
Ksp632I MaeI MaeII MamI MboII
MfeI MluI MluNI MroI MscI
MslI MunI NaeI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PpuMI PshBI Psp1406I PvuI RcaI
RsrII SacI SalI SapI SbfI
ScaI SexAI SfaNI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TaqI TfiI Tsp509I
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
###################################
AccII 84 CGCG CG^CG
AlwI 79 GATCC GGATC(4/5)
AlwNI 250 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AvaI 161 CCCGAG C^[CT]CG[GA]G
AvaII 258 GGACC G^G[AT]CC
BanII 87 GGGCTC G[GA]GC[CT]^C
BbvCI 129 GCTGAGG CCTCAGC(-5/-2)
BmyI 87 GGGCTC G[AGT]GC[ACT]^C
BsiYI 263 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 263 CCAGCCACAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsoBI 161 CCCGAG C^[CT]CG[GA]G
Bsp1286I 87 GGGCTC G[AGT]GC[ACT]^C
BspMI 245 ACCTGC ACCTGC(4/8)
BsrFI 63 GCCGGT [GA]^CCGG[CT]
BsrI 39 ACTGG ACTGG(1/-1)
BstAPI 60 GCAGCCGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstUI 84 CGCG CG^CG
BstYI 78 AGATCC [GA]^GATC[CT]
Cfr10I 63 GCCGGT [GA]^CCGG[CT]
DdeI 130 CTGAG C^T[ACGT]AG
DpnII 79 GATC ^GATC
DsaI 83 CCGCGG C^C[GA][CT]GG
EaeI 309 TGGCCG [CT]^GGCC[GA]
HgaI 57 GACGC GACGC(5/10)
HinfI 157 GAGTC G^A[ACGT]TC
KspI 83 CCGCGG CCGC^GG
MaeIII 278 GTGAC ^GT[ACGT]AC
MboI 79 GATC ^GATC
MflI 78 AGATCC [GA]^GATC[CT]
MseI 239 TTAA T^TAA
MvnI 84 CGCG CG^CG
NdeII 79 GATC ^GATC
NlaIII 291 CATG CATG^
PleI 157 GAGTC GAGTC(4/5)
PshAI 224 GACGAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuII 127 CAGCTG CAG^CTG
SacII 83 CCGCGG CCGC^GG
Sau3AI 79 GATC ^GATC
Tru9I 239 TTAA T^TAA
Tsp45I 278 GTGAC ^GT[GC]AC
TspRI 318 CAGTG CA[GC]TG[ACGT][ACGT]^
XhoII 78 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 37 GTAC GT^AC
336 GTAC
AluI 128 AGCT AG^CT
213 AGCT
BglI 117 GCCCCGTAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
178 GCCCTGCTGGC
BseRI 109 GAGGAG GAGGAG(10/8)
325 GAGGAG
BsmFI 284 GGGAC GGGAC(10/14)
159 GTCCC
BstNI 171 CCTGG CC^[AT]GG
255 CCTGG
Cfr13I 204 GGCCC G^G[ACGT]CC
258 GGACC
EcoRII 171 CCTGG ^CC[AT]GG
255 CCTGG
HaeIII 204 GGCC GG^CC
310 GGCC
MvaI 171 CCTGG CC^[AT]GG
255 CCTGG
PstI 97 CTGCAG CTGCA^G
247 CTGCAG
RsaI 37 GTAC GT^AC
336 GTAC
Sau96I 204 GGCCC G^G[ACGT]CC
258 GGACC
SfcI 97 CTGCAG C^T[GA][CT]AG
247 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AcyI BanI BbeI BbvI
BcnI BsaHI BsaJI BssKI BstF5I
Cac8I CfoI FauI Fnu4HI FokI
HaeII HapII HhaI Hin1I HinP1I
HpaII ItaI KasI MnlI MspA1I
MspI MwoI NarI NciI NlaIV
ScrFI SfoI TseI