Restriction map for
pFN21AE1521
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# NO CUT ENZYMES ( 151 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AgeI AhdI AlwNI AocI
Aor51HI ApaI AscI AseI AsnI
Asp700I Asp718I AspEI AspI AvaI
AviII AvrII BalI BanI BanII
BbeI BbrPI BcgI BciVI BclI
BcnI BfrI BglI BlnI BlpI
BpmI Bpu1102I BsaAI BsaHI BsaI
BsaWI BseAI BsgI BsiEI BsiWI
BsiYI BslI BsmBI BsmI BsoBI
BspDI BspEI BspHI BsrBI BsrDI
BsrFI BssHII BssSI BstAPI Bsu36I
CelII CfoI Cfr10I ClaI CpoI
DraIII DrdI DsaI EaeI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoNI EcoRV EcoT22I FauI FbaI
FseI FspI HaeII HapII HhaI
Hin1I HinP1I HpaI HpaII KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MspI MunI MwoI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RcaI RsrII
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AccI 202 GTATAC GT^[AC][GT]AC
AccII 674 CGCG CG^CG
AflIII 979 ACATGT A^C[GA][CT]GT
Alw44I 12 GTGCAC G^TGCAC
ApaLI 12 GTGCAC G^TGCAC
AspHI 12 GTGCAC G[AT]GC[AT]^C
BamHI 3 GGATCC G^GATCC
BbsI 951 GAAGAC GAAGAC(2/6)
BbvCI 715 CCTCAGC CCTCAGC(-5/-2)
BbvI 67 GCTGC GCAGC(8/12)
BfaI 157 CTAG C^TAG
BglII 916 AGATCT A^GATCT
BmyI 12 GTGCAC G[AGT]GC[ACT]^C
BpuAI 951 GAAGAC GAAGAC(2/6)
BsaBI 360 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaJI 739 CCTTGG C^C[ACGT][ACGT]GG
BseRI 71 CTCCTC GAGGAG(10/8)
BsiHKAI 12 GTGCAC G[AT]GC[AT]^C
BsmFI 21 GGGAC GGGAC(10/14)
Bsp1286I 12 GTGCAC G[AGT]GC[ACT]^C
Bsp1407I 452 TGTACA T^GTACA
BspLU11I 979 ACATGT A^CATGT
BsrGI 452 TGTACA T^GTACA
BsrI 9 CCAGT ACTGG(1/-1)
BssKI 177 CCAGG ^CC[ACGT]GG
Bst1107I 202 GTATAC GTA^TAC
BstBI 948 TTCGAA TT^CGAA
BstEII 484 GGTTACC G^GT[ACGT]ACC
BstNI 177 CCAGG CC^[AT]GG
BstPI 484 GGTTACC G^GT[ACGT]ACC
BstUI 674 CGCG CG^CG
BstZ17I 202 GTATAC GTA^TAC
Cac8I 396 GCTTGC GC[ACGT]^[ACGT]GC
DraI 940 TTTAAA TTT^AAA
DraII 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EarI 868 GAAGAG CTCTTC(1/4)
EcoO109I 710 AGGACCC [GA]G^G[ACGT]CC[CT]
EcoO65I 484 GGTTACC G^GT[ACGT]ACC
EcoRII 177 CCAGG ^CC[AT]GG
EcoT14I 739 CCTTGG C^C[AT][AT]GG
HgaI 672 GACGC GACGC(5/10)
HincII 410 GTCAAC GT[CT]^[GA]AC
HindII 410 GTCAAC GT[CT]^[GA]AC
HindIII 86 AAGCTT A^AGCTT
HphI 688 TCACC GGTGA(8/7)
Ksp632I 868 GAAGAG CTCTTC(1/4)
MaeI 157 CTAG C^TAG
MaeII 593 ACGT A^CGT
MamI 360 GATTCGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 941 TTAA T^TAA
MspA1I 749 CAGCTG C[AC]G^C[GT]G
MvaI 177 CCAGG CC^[AT]GG
MvnI 674 CGCG CG^CG
NspI 979 ACATGT [GA]CATG^[CT]
NspV 948 TTCGAA TT^CGAA
PpuMI 710 AGGACCC [GA]G^G[AT]CC[CT]
PstI 104 CTGCAG CTGCA^G
PvuII 749 CAGCTG CAG^CTG
ScrFI 177 CCAGG CC^[ACGT]GG
SfaNI 365 GCATC GCATC(5/9)
SfuI 948 TTCGAA TT^CGAA
SspBI 452 TGTACA T^GTACA
StyI 739 CCTTGG C^C[AT][AT]GG
Tru9I 941 TTAA T^TAA
TseI 67 GCTGC G^C[AT]GC
Tsp45I 577 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AlwI 4 GATCC GGATC(4/5)
3 GGATC
BsmAI 100 GAGAC GTCTC(1/5)
285 GAGAC
BspMI 110 ACCTGC ACCTGC(4/8)
466 ACCTGC
BstF5I 333 CATCC GGATG(2/0)
366 CATCC
BstXI 239 CCAAGTATATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
851 CCAACACAATGG
BstYI 3 GGATCC [GA]^GATC[CT]
916 AGATCT
EcoRI 378 GAATTC G^AATTC
528 GAATTC
FokI 333 CATCC GGATG(9/13)
366 CATCC
MflI 3 GGATCC [GA]^GATC[CT]
916 AGATCT
MslI 351 CACAGGAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
956 CATCTTCATG
SfcI 104 CTGCAG C^T[GA][CT]AG
698 CTATAG
SmlI 566 CTCAAG C^T[CT][GA]AG
706 CTTGAG
TaqI 539 TCGA T^CGA
949 TCGA
TthHB8I 539 TCGA T^CGA
949 TCGA
XhoII 3 GGATCC [GA]^GATC[CT]
916 AGATCT
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII Cfr13I DdeI DpnII Fnu4HI
HaeIII HinfI ItaI MaeIII MboI
MboII MnlI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I TfiI Tsp509I
TspRI