Restriction map for
pFN21AE4548
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# NO CUT ENZYMES ( 119 enzymes) #
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AatII AccI AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwI Aor51HI ApaI ApaLI AscI
Asp700I AspEI AspHI AspI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiHKAI
BsiWI BsmBI BsmI Bsp1407I BspEI
BspHI BspLU11I BsrFI BsrGI BssHII
Bst1107I BstBI BstYI BstZ17I CelII
Cfr10I CpoI DrdI Eam1105I EarI
Eco47III EcoT22I FseI FspI HaeII
Hin1I HincII HindII HpaI KasI
Ksp632I MaeII MamI MflI MluI
MluNI MroI MscI NaeI NarI
NdeI NgoMIV NotI NsiI NspV
PacI PflFI PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PvuI
RcaI RsrII SacI SalI SapI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI SspBI
SspI SwaI Tth111I Van91I XbaI
XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AlwNI 1223 CAGGTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 508 CCTGAGG CC^T[ACGT]AGG
AseI 1305 ATTAAT AT^TAAT
AsnI 1305 ATTAAT AT^TAAT
AvaI 52 CTCGAG C^[CT]CG[GA]G
BbvCI 548 CCTCAGC CCTCAGC(-5/-2)
BclI 1854 TGATCA T^GATCA
BglI 37 GCCCCATGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpmI 418 CTGGAG CTGGAG(16/14)
BseRI 421 GAGGAG GAGGAG(10/8)
BsiEI 243 CGGCCG CG[GA][CT]^CG
BsmAI 746 GAGAC GTCTC(1/5)
BsoBI 52 CTCGAG C^[CT]CG[GA]G
BspDI 1348 ATCGAT AT^CGAT
BspMI 657 GCAGGT ACCTGC(4/8)
BsrBI 125 CCGCTC CCGCTC(-3/-3)
BssSI 95 CACGAG CACGAG(-5/-1)
BstAPI 1765 GCAATTGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 364 GGTGACC G^GT[ACGT]ACC
BstPI 364 GGTGACC G^GT[ACGT]ACC
BstXI 686 CCATGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 508 CCTGAGG CC^T[ACGT]AGG
ClaI 1348 ATCGAT AT^CGAT
DraII 1224 AGGTCCT [GA]G^G[ACGT]CC[CT]
DraIII 1858 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 243 CGGCCG C^GGCCG
EclXI 243 CGGCCG C^GGCCG
Eco52I 243 CGGCCG C^GGCCG
Eco81I 508 CCTGAGG CC^T[ACGT]AGG
EcoNI 1216 CCTCCCACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 1224 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 364 GGTGACC G^GT[ACGT]ACC
EcoRI 1669 GAATTC G^AATTC
FbaI 1854 TGATCA T^GATCA
HgaI 8 GCGTC GACGC(5/10)
HindIII 1445 AAGCTT A^AGCTT
KspI 205 CCGCGG CCGC^GG
MfeI 1766 CAATTG C^AATTG
MslI 687 CATGACCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 1766 CAATTG C^AATTG
NheI 1825 GCTAGC G^CTAGC
NruI 106 TCGCGA TCG^CGA
PaeR7I 52 CTCGAG C^TCGAG
PpuMI 1224 AGGTCCT [GA]G^G[AT]CC[CT]
PshBI 1305 ATTAAT AT^TAAT
PvuII 1205 CAGCTG CAG^CTG
SacII 205 CCGCGG CCGC^GG
SbfI 1552 CCTGCAGG CCTGCA^GG
ScaI 1546 AGTACT AGT^ACT
SfaNI 270 GCATC GCATC(5/9)
Sse8387I 1552 CCTGCAGG CCTGCA^GG
StuI 521 AGGCCT AGG^CCT
XcmI 683 CCACCATGACCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 52 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 17 enzymes) #
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Acc65I 81 GGTACC G^GTACC
457 GGTACC
Asp718I 81 GGTACC G^GTACC
457 GGTACC
BanII 23 GGGCTC G[GA]GC[CT]^C
1883 GAGCCC
BbsI 260 GTCTTC GAAGAC(2/6)
1255 GTCTTC
BpuAI 260 GTCTTC GAAGAC(2/6)
1255 GTCTTC
BsrDI 284 CATTGC GCAATG(2/0)
1608 CATTGC
CfoI 6 GCGC GCG^C
16 GCGC
EaeI 243 CGGCCG [CT]^GGCC[GA]
977 TGGCCG
EcoRV 1162 GATATC GAT^ATC
1264 GATATC
EcoT14I 40 CCATGG C^C[AT][AT]GG
692 CCATGG
HhaI 6 GCGC GCG^C
16 GCGC
HinP1I 6 GCGC G^CGC
16 GCGC
KpnI 81 GGTACC GGTAC^C
457 GGTACC
NcoI 40 CCATGG C^CATGG
692 CCATGG
SmlI 52 CTCGAG C^T[CT][GA]AG
1748 CTCAAG
StyI 40 CCATGG C^C[AT][AT]GG
692 CCATGG
TfiI 544 GATTC G^A[AT]TC
1148 GAATC
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# 3 OR MORE CUT ENZYMES ( 68 enzymes) #
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AccII AciI AcsI AfaI AluI
ApoI AvaII BanI BbvI BcnI
BfaI BmyI BsaJI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstUI Cac8I Cfr13I DdeI
DpnII DraI DsaI EcoRII FauI
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeIII
MboI MboII MnlI MseI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV NspI PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfcI TaqI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I