Restriction map for
pFN21AE3632
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AvaI AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BclI
BfrI BglI BglII BlnI BlpI
BpmI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmBI BsmFI BsmI
BsoBI Bsp1407I BspDI BspEI BspLU11I
BspMI BsrBI BsrDI BsrFI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstUI BstXI BstYI
BstZ17I Bsu36I Cac8I CelII CfoI
Cfr10I ClaI CpoI DraI DraII
DraIII DrdI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoNI EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HhaI
Hin1I HinP1I HindIII HphI KasI
KpnI Ksp632I KspI MamI MfeI
MflI MluI MluNI MroI MscI
MslI MunI MvnI NaeI NarI
NdeI NgoMIV NheI NotI NruI
NsiI NspI NspV PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
PvuII RsrII SacI SacII SalI
SapI SbfI ScaI SfcI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI TaqI TthHB8I
XbaI XhoI XhoII XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AcsI 561 GAATTC [GA]^AATT[CT]
AlwI 298 GGATC GGATC(4/5)
ApoI 561 GAATTC [GA]^AATT[CT]
Asp700I 211 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 232 GTGCTC G[AT]GC[AT]^C
AspI 84 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BcnI 22 CCGGG CC^[GC]GG
BfaI 229 CTAG C^TAG
BsaI 131 GGTCTC GGTCTC(1/5)
BsiHKAI 232 GTGCTC G[AT]GC[AT]^C
DpnII 299 GATC ^GATC
EcoRI 561 GAATTC G^AATTC
FauI 310 GCGGG CCCGC(4/6)
HapII 22 CCGG C^CGG
HgaI 225 GCGTC GACGC(5/10)
HincII 600 GTTAAC GT[CT]^[GA]AC
HindII 600 GTTAAC GT[CT]^[GA]AC
HpaI 600 GTTAAC GTT^AAC
HpaII 22 CCGG C^CGG
MaeI 229 CTAG C^TAG
MaeII 38 ACGT A^CGT
MaeIII 682 GTGAC ^GT[ACGT]AC
MboI 299 GATC ^GATC
MboII 278 TCTTC GAAGA(8/7)
MspA1I 78 CCGCTG C[AC]G^C[GT]G
MspI 22 CCGG C^CGG
NciI 22 CCGGG CC^[GC]GG
NcoI 616 CCATGG C^CATGG
NdeII 299 GATC ^GATC
PflFI 84 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 13 GACTC GAGTC(4/5)
Sau3AI 299 GATC ^GATC
SexAI 85 ACCTGGT A^CC[AT]GGT
Tsp45I 682 GTGAC ^GT[GC]AC
Tth111I 84 GACCTGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 678 CCATGTGACCATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 211 GAACCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
BbvI 373 GCTGC GCAGC(8/12)
376 GCTGC
BsmAI 132 GTCTC GTCTC(1/5)
705 GTCTC
BspHI 357 TCATGA T^CATGA
893 TCATGA
BsrI 743 ACTGG ACTGG(1/-1)
555 CCAGT
BstNI 9 CCTGG CC^[AT]GG
86 CCTGG
DsaI 47 CCACGG C^C[GA][CT]GG
616 CCATGG
EcoRII 9 CCTGG ^CC[AT]GG
86 CCTGG
EcoT14I 136 CCTTGG C^C[AT][AT]GG
616 CCATGG
Fnu4HI 373 GCTGC GC^[ACGT]GC
376 GCTGC
ItaI 373 GCTGC GC^[ACGT]GC
376 GCTGC
MvaI 9 CCTGG CC^[AT]GG
86 CCTGG
PflMI 330 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
826 CCAAAAGCTGG
RcaI 357 TCATGA T^CATGA
893 TCATGA
StyI 136 CCTTGG C^C[AT][AT]GG
616 CCATGG
TfiI 621 GATTC G^A[AT]TC
669 GATTC
TseI 373 GCTGC G^C[AT]GC
376 GCTGC
Van91I 330 CCAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
826 CCAAAAGCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 29 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I Cfr13I DdeI FokI
HaeIII HinfI MnlI MseI MwoI
NlaIII NlaIV RsaI Sau96I ScrFI
SfaNI Tru9I Tsp509I TspRI