Restriction map for
pFN21AE3596
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# NO CUT ENZYMES ( 96 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AhdI Alw44I Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspI AviII BamHI
BciVI BclI BfrI BglII BsaBI
BseAI BsiWI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstPI
BstXI BstZ17I ClaI CpoI DraI
Eam1105I EarI Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HincII HindII HpaI KpnI Ksp632I
MamI MfeI MluI MroI MseI
MunI NcoI NdeI NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PmeI PshBI Psp1406I PvuI
RcaI RsrII SalI SapI SbfI
SfuI SnaBI SpeI Sse8387I SspBI
SspI SwaI Tru9I Tth111I XbaI
XhoI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AgeI 824 ACCGGT A^CCGGT
AocI 1123 CCTCAGG CC^T[ACGT]AGG
Asp700I 381 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 1066 CCCGGG C^[CT]CG[GA]G
AvrII 2325 CCTAGG C^CTAGG
BalI 2246 TGGCCA TGG^CCA
BbeI 1591 GGCGCC GGCGC^C
BbrPI 543 CACGTG CAC^GTG
BbsI 1773 GAAGAC GAAGAC(2/6)
BcgI 797 GCATCACCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlnI 2325 CCTAGG C^CTAGG
BlpI 838 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 838 GCTGAGC GC^T[ACGT]AGC
BpuAI 1773 GAAGAC GAAGAC(2/6)
BsaAI 543 CACGTG [CT]AC^GT[GA]
BsaI 1428 GGTCTC GGTCTC(1/5)
BsaWI 824 ACCGGT [AT]^CCGG[AT]
BsmBI 1470 CGTCTC CGTCTC(1/5)
BsmI 1047 GAATGC GAATGC(1/-1)
BsoBI 1066 CCCGGG C^[CT]CG[GA]G
BstYI 812 AGATCC [GA]^GATC[CT]
Bsu36I 1123 CCTCAGG CC^T[ACGT]AGG
CelII 838 GCTGAGC GC^T[ACGT]AGC
DraIII 543 CACGTGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 1123 CCTCAGG CC^T[ACGT]AGG
KasI 1591 GGCGCC G^GCGCC
MflI 812 AGATCC [GA]^GATC[CT]
MluNI 2246 TGGCCA TGG^CCA
MscI 2246 TGGCCA TGG^CCA
NarI 1591 GGCGCC GG^CGCC
PflMI 270 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 824 ACCGGT A^CCGGT
PmaCI 543 CACGTG CAC^GTG
PmlI 543 CACGTG CAC^GTG
PshAI 1516 GACAGTTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
ScaI 413 AGTACT AGT^ACT
SexAI 269 ACCAGGT A^CC[AT]GGT
SfiI 2247 GGCCAGGCTGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SfoI 1591 GGCGCC GGC^GCC
SgrAI 823 CACCGGTG C[GA]^CCGG[CT]G
SmaI 1066 CCCGGG CCC^GGG
SphI 770 GCATGC GCATG^C
TfiI 190 GATTC G^A[AT]TC
Van91I 270 CCAGGTTATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1787 CCAAAGGGTTTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 812 AGATCC [GA]^GATC[CT]
XmaCI 1066 CCCGGG C^CCGGG
XmaI 1066 CCCGGG C^CCGGG
XmnI 381 GAAGAAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AcsI 310 GAATTT [GA]^AATT[CT]
513 GAATTT
AcyI 1591 GGCGCC G[GA]^CG[CT]C
1719 GGCGTC
AfaI 414 GTAC GT^AC
642 GTAC
AlwI 762 GATCC GGATC(4/5)
813 GATCC
ApoI 310 GAATTT [GA]^AATT[CT]
513 GAATTT
BbvCI 2165 CCTCAGC CCTCAGC(-5/-2)
1767 GCTGAGG
BsaHI 1591 GGCGCC G[GA]^CG[CT]C
1719 GGCGTC
BsiEI 404 CGGCCG CG[GA][CT]^CG
2000 CGGCCG
BstAPI 1489 GCAAGTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1971 GCAGAGGCTGC
DrdI 963 GACGGTGGAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
2145 GACCGGCGCGTC
EagI 404 CGGCCG C^GGCCG
2000 CGGCCG
EclXI 404 CGGCCG C^GGCCG
2000 CGGCCG
Eco52I 404 CGGCCG C^GGCCG
2000 CGGCCG
EcoT14I 1184 CCTTGG C^C[AT][AT]GG
2325 CCTAGG
HaeII 921 GGCGCT [GA]GCGC^[CT]
1591 GGCGCC
Hin1I 1591 GGCGCC G[GA]^CG[CT]C
1719 GGCGTC
HindIII 235 AAGCTT A^AGCTT
262 AAGCTT
NaeI 1924 GCCGGC GCC^GGC
2034 GCCGGC
NgoMIV 1924 GCCGGC G^CCGGC
2034 GCCGGC
NspI 290 ACATGC [GA]CATG^[CT]
770 GCATGC
RsaI 414 GTAC GT^AC
642 GTAC
StuI 1751 AGGCCT AGG^CCT
1905 AGGCCT
StyI 1184 CCTTGG C^C[AT][AT]GG
2325 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
AvaII BanI BanII BbvI BcnI
BfaI BglI BmyI BpmI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
BstUI Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EaeI
EcoNI EcoO109I EcoRII FauI Fnu4HI
FokI HaeIII HapII HgaI HhaI
HinP1I HinfI HpaII HphI ItaI
KspI MaeI MaeII MaeIII MboI
MboII MnlI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PpuMI PstI
PvuII SacI SacII Sau3AI Sau96I
ScrFI SfaNI SfcI SmlI TaqI
TseI Tsp45I Tsp509I TspRI TthHB8I