Restriction map for
pFN21AE4590
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# NO CUT ENZYMES ( 85 enzymes) #
##################################
AatII AccII AclI AflII AgeI
AhdI ApaI AscI AseI AsnI
AspEI AspI AviII AvrII BbvCI
BcgI BfrI BlnI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrBI
BsrFI BsrGI BssHII BssSI BstEII
BstPI BstUI Cfr10I ClaI CpoI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco52I EcoO65I EcoRV FseI
FspI HgaI HpaI KspI MfeI
MluI MunI MvnI NaeI NcoI
NgoMIV NheI NotI NruI PacI
PaeR7I PflFI PinAI PmeI PshBI
Psp1406I PvuI RsrII SacII SalI
SapI SbfI SfiI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SwaI
Tth111I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 60 enzymes) #
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Acc65I 1895 GGTACC G^GTACC
AccI 870 GTATAC GT^[AC][GT]AC
AccIII 34 TCCGGA T^CCGGA
AcyI 1926 GGCGCC G[GA]^CG[CT]C
AflIII 1319 ACACGT A^C[GA][CT]GT
Alw44I 3148 GTGCAC G^TGCAC
AlwNI 2357 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 2821 CCTGAGG CC^T[ACGT]AGG
Aor51HI 400 AGCGCT AGC^GCT
ApaLI 3148 GTGCAC G^TGCAC
Asp718I 1895 GGTACC G^GTACC
AvaI 90 CTCGGG C^[CT]CG[GA]G
BanII 365 GAGCTC G[GA]GC[CT]^C
BbeI 1926 GGCGCC GGCGC^C
BciVI 2479 GTATCC GTATCC(6/5)
BcnI 645 CCCGG CC^[GC]GG
BglI 1325 GCCTCAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 958 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 958 GCTCAGC GC^T[ACGT]AGC
BsaHI 1926 GGCGCC G[GA]^CG[CT]C
BsaWI 34 TCCGGA [AT]^CCGG[AT]
BseAI 34 TCCGGA T^CCGGA
BsmFI 3103 GTCCC GGGAC(10/14)
BsoBI 90 CTCGGG C^[CT]CG[GA]G
BspEI 34 TCCGGA T^CCGGA
BspHI 449 TCATGA T^CATGA
Bst1107I 870 GTATAC GTA^TAC
BstBI 1943 TTCGAA TT^CGAA
BstZ17I 870 GTATAC GTA^TAC
Bsu36I 2821 CCTGAGG CC^T[ACGT]AGG
CelII 958 GCTCAGC GC^T[ACGT]AGC
DraI 1831 TTTAAA TTT^AAA
Eco47III 400 AGCGCT AGC^GCT
Eco81I 2821 CCTGAGG CC^T[ACGT]AGG
EcoNI 3270 CCTGTCTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 2007 GAATTC G^AATTC
FauI 3252 GCGGG CCCGC(4/6)
Hin1I 1926 GGCGCC G[GA]^CG[CT]C
KasI 1926 GGCGCC G^GCGCC
KpnI 1895 GGTACC GGTAC^C
MroI 34 TCCGGA T^CCGGA
MslI 2781 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 961 CAGCTG C[AC]G^C[GT]G
NarI 1926 GGCGCC GG^CGCC
NciI 645 CCCGG CC^[GC]GG
NspV 1943 TTCGAA TT^CGAA
PflMI 2929 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 374 AGGACCC [GA]G^G[AT]CC[CT]
PshAI 2186 GACGGGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1600 CTGCAG CTGCA^G
PvuII 961 CAGCTG CAG^CTG
RcaI 449 TCATGA T^CATGA
SacI 365 GAGCTC GAGCT^C
ScaI 2581 AGTACT AGT^ACT
SexAI 302 ACCTGGT A^CC[AT]GGT
SfoI 1926 GGCGCC GGC^GCC
SfuI 1943 TTCGAA TT^CGAA
StuI 2196 AGGCCT AGG^CCT
Van91I 2929 CCACCTGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 2454 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 38 enzymes) #
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AspHI 365 GAGCTC G[AT]GC[AT]^C
3148 GTGCAC
AvaII 375 GGACC G^G[AT]CC
1979 GGACC
BamHI 879 GGATCC G^GATCC
2827 GGATCC
BbrPI 1320 CACGTG CAC^GTG
2611 CACGTG
BbsI 799 GAAGAC GAAGAC(2/6)
2326 GAAGAC
BmyI 365 GAGCTC G[AGT]GC[ACT]^C
3148 GTGCAC
BpmI 2160 CTCCAG CTGGAG(16/14)
2486 CTCCAG
BpuAI 799 GAAGAC GAAGAC(2/6)
2326 GAAGAC
BsaAI 1320 CACGTG [CT]AC^GT[GA]
2611 CACGTG
BsaI 1712 GAGACC GGTCTC(1/5)
2697 GGTCTC
BsiHKAI 365 GAGCTC G[AT]GC[AT]^C
3148 GTGCAC
BsmI 504 GAATGC GAATGC(1/-1)
933 GAATGC
Bsp1286I 365 GAGCTC G[AGT]GC[ACT]^C
3148 GTGCAC
BspMI 1416 ACCTGC ACCTGC(4/8)
720 GCAGGT
BsrDI 3030 CATTGC GCAATG(2/0)
2135 GCAATG
BstAPI 508 GCAGCCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2988 GCAAGGAGTGC
BstXI 1298 CCAACATCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1451 CCAAACTCCTGG
CfoI 401 GCGC GCG^C
1927 GCGC
DraII 94 GGGGCCT [GA]G^G[ACGT]CC[CT]
374 AGGACCC
EcoO109I 94 GGGGCCT [GA]G^G[ACGT]CC[CT]
374 AGGACCC
EcoT14I 775 CCTTGG C^C[AT][AT]GG
2897 CCAAGG
EcoT22I 224 ATGCAT ATGCA^T
2788 ATGCAT
HaeII 400 AGCGCT [GA]GCGC^[CT]
1926 GGCGCC
HapII 35 CCGG C^CGG
646 CCGG
HhaI 401 GCGC GCG^C
1927 GCGC
HinP1I 401 GCGC G^CGC
1927 GCGC
HincII 1134 GTCAAC GT[CT]^[GA]AC
2553 GTCAAC
HindII 1134 GTCAAC GT[CT]^[GA]AC
2553 GTCAAC
HpaII 35 CCGG C^CGG
646 CCGG
MaeII 1321 ACGT A^CGT
2612 ACGT
MspI 35 CCGG C^CGG
646 CCGG
NdeI 491 CATATG CA^TATG
1444 CATATG
NsiI 224 ATGCAT ATGCA^T
2788 ATGCAT
NspI 737 ACATGC [GA]CATG^[CT]
2790 GCATGT
PmaCI 1320 CACGTG CAC^GTG
2611 CACGTG
PmlI 1320 CACGTG CAC^GTG
2611 CACGTG
SmlI 1819 CTTGAG C^T[CT][GA]AG
2815 CTTGAG
StyI 775 CCTTGG C^C[AT][AT]GG
2897 CCAAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI Asp700I BalI BanI BbvI
BclI BfaI BglII BsaBI BsaJI
BseRI BsgI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EaeI
EarI EcoRII FbaI Fnu4HI FokI
HaeIII HindIII HinfI HphI ItaI
Ksp632I MaeI MaeIII MamI MboI
MboII MflI MluNI MnlI MscI
MseI MvaI MwoI NdeII NlaIII
NlaIV PleI RsaI Sau3AI Sau96I
ScrFI SfaNI SfcI SpeI SspI
TaqI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI TthHB8I XhoII XmnI