Restriction map for
pFN21AE1403
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# NO CUT ENZYMES ( 130 enzymes) #
##################################
Acc65I AccI AccIII AclI AflII
Alw44I AlwNI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AvaI AviII AvrII BamHI BanI
BbeI BbvCI BcgI BciVI BfaI
BfrI BglI BlnI BlpI Bpu1102I
BsaBI BseAI BseRI BsiEI BsiWI
BsmBI BsmFI BsmI BsoBI BspDI
BspEI BspLU11I BspMI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstXI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI DsaI EagI
EarI EclXI Eco47III Eco52I EcoNI
EcoO65I EcoRV EcoT22I FseI FspI
HaeII HincII HindII HindIII HpaI
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MroI MspA1I
MunI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PmeI
PshBI Psp1406I PstI PvuI PvuII
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SmaI SnaBI SpeI
SphI Sse8387I StuI SwaI XbaI
XcmI XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 64 enzymes) #
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AatII 244 GACGTC GACGT^C
AccII 485 CGCG CG^CG
AcyI 244 GACGTC G[GA]^CG[CT]C
AflIII 701 ACACGT A^C[GA][CT]GT
AhdI 1099 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 1028 CCTCAGG CC^T[ACGT]AGG
AspEI 1099 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 450 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 263 TGGCCA TGG^CCA
BanII 616 GAGCCC G[GA]GC[CT]^C
BbrPI 348 CACGTG CAC^GTG
BbsI 550 GTCTTC GAAGAC(2/6)
BclI 203 TGATCA T^GATCA
BcnI 1046 CCGGG CC^[GC]GG
BglII 986 AGATCT A^GATCT
BpuAI 550 GTCTTC GAAGAC(2/6)
BsaAI 348 CACGTG [CT]AC^GT[GA]
BsaHI 244 GACGTC G[GA]^CG[CT]C
BsaI 1084 GAGACC GGTCTC(1/5)
BsgI 919 CTGCAC GTGCAG(16/14)
Bsp1407I 441 TGTACA T^GTACA
BspHI 686 TCATGA T^CATGA
BsrBI 3 GAGCGG CCGCTC(-3/-3)
BsrGI 441 TGTACA T^GTACA
BstUI 485 CGCG CG^CG
BstYI 986 AGATCT [GA]^GATC[CT]
Bsu36I 1028 CCTCAGG CC^T[ACGT]AGG
Cac8I 726 GCAAGC GC[ACGT]^[ACGT]GC
CfoI 486 GCGC GCG^C
DraII 995 AGGACCT [GA]G^G[ACGT]CC[CT]
EaeI 263 TGGCCA [CT]^GGCC[GA]
Eam1105I 1099 GACAATGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1028 CCTCAGG CC^T[ACGT]AGG
EcoO109I 995 AGGACCT [GA]G^G[ACGT]CC[CT]
EcoRI 735 GAATTC G^AATTC
FbaI 203 TGATCA T^GATCA
HgaI 1105 GCGTC GACGC(5/10)
HhaI 486 GCGC GCG^C
Hin1I 244 GACGTC G[GA]^CG[CT]C
HinP1I 486 GCGC G^CGC
MflI 986 AGATCT [GA]^GATC[CT]
MluNI 263 TGGCCA TGG^CCA
MscI 263 TGGCCA TGG^CCA
MseI 386 TTAA T^TAA
MvnI 485 CGCG CG^CG
MwoI 319 GCCACCGGTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NciI 1046 CCGGG CC^[GC]GG
PflFI 450 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 620 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 348 CACGTG CAC^GTG
PmlI 348 CACGTG CAC^GTG
PpuMI 995 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 240 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 686 TCATGA T^CATGA
SgrAI 321 CACCGGTG C[GA]^CCGG[CT]G
SmlI 649 CTTGAG C^T[CT][GA]AG
SspBI 441 TGTACA T^GTACA
SspI 190 AATATT AAT^ATT
TaqI 35 TCGA T^CGA
Tru9I 386 TTAA T^TAA
Tth111I 450 GACTAAGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 35 TCGA T^CGA
Van91I 620 CCATCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 986 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AgeI 322 ACCGGT A^CCGGT
425 ACCGGT
AlwI 470 GATCC GGATC(4/5)
857 GATCC
AspHI 105 GTGCTC G[AT]GC[AT]^C
940 GTGCTC
BsaWI 322 ACCGGT [AT]^CCGG[AT]
425 ACCGGT
BsiHKAI 105 GTGCTC G[AT]GC[AT]^C
940 GTGCTC
BsmAI 1084 GAGAC GTCTC(1/5)
610 GTCTC
BsrDI 267 CATTGC GCAATG(2/0)
341 GCAATG
BsrFI 322 ACCGGT [GA]^CCGG[CT]
425 ACCGGT
BsrI 41 CCAGT ACTGG(1/-1)
1079 CCAGT
Cfr10I 322 ACCGGT [GA]^CCGG[CT]
425 ACCGGT
EcoT14I 573 CCAAGG C^C[AT][AT]GG
719 CCTTGG
FauI 5 GCGGG CCCGC(4/6)
392 GCGGG
HphI 201 GGTGA GGTGA(8/7)
206 TCACC
MslI 414 CATTAGCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
768 CATAGCTGTG
NlaIV 464 GGCTCC GG[ACGT]^[ACGT]CC
615 GGAGCC
PinAI 322 ACCGGT A^CCGGT
425 ACCGGT
PleI 37 GACTC GAGTC(4/5)
312 GAGTC
SfaNI 396 GATGC GCATC(5/9)
368 GCATC
StyI 573 CCAAGG C^C[AT][AT]GG
719 CCTTGG
Tsp45I 826 GTGAC ^GT[GC]AC
898 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 44 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AvaII BbvI BmyI BpmI BsaJI
BsiYI BslI Bsp1286I BssKI BstF5I
BstNI Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII ItaI MaeII MaeIII MboI
MboII MnlI MspI MvaI NdeII
NlaIII RsaI Sau3AI Sau96I ScrFI
TfiI TseI Tsp509I TspRI