Restriction map for
pFN21AE6310
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# NO CUT ENZYMES ( 152 enzymes) #
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AatII Acc65I AccIII AclI AcyI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AvaI AvaII AviII AvrII
BamHI BbeI BbrPI BcgI BcnI
BfrI BglII BlnI BlpI BpmI
Bpu1102I BsaAI BsaBI BsaHI BsaWI
BseAI BsgI BsiEI BsiWI BsmI
BsoBI BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BssHII Bst1107I BstAPI
BstBI BstEII BstPI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DraIII DrdI DsaI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV EcoT22I FauI
FseI FspI HaeII HgaI Hin1I
HincII HindII HindIII HpaI KasI
KpnI KspI MaeII MamI MfeI
MluI MroI MunI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SphI Sse8387I
SspI StuI SwaI TaqI Tth111I
TthHB8I Van91I XbaI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AccI 508 GTAGAC GT^[AC][GT]AC
AccII 1128 CGCG CG^CG
ApaI 400 GGGCCC GGGCC^C
AspHI 1222 GAGCAC G[AT]GC[AT]^C
BalI 485 TGGCCA TGG^CCA
BanI 360 GGCACC G^G[CT][GA]CC
BbsI 741 GAAGAC GAAGAC(2/6)
BciVI 328 GTATCC GTATCC(6/5)
BclI 870 TGATCA T^GATCA
BglI 685 GCCATGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 741 GAAGAC GAAGAC(2/6)
BsiHKAI 1222 GAGCAC G[AT]GC[AT]^C
BsmBI 1107 CGTCTC CGTCTC(1/5)
BsmFI 49 GGGAC GGGAC(10/14)
Bsp1407I 548 TGTACA T^GTACA
BspHI 873 TCATGA T^CATGA
BsrGI 548 TGTACA T^GTACA
BssSI 1219 CACGAG CACGAG(-5/-1)
BstF5I 168 CATCC GGATG(2/0)
BstUI 1128 CGCG CG^CG
BstXI 179 CCATGAATCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 1053 GCGC GCG^C
DraII 1097 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO109I 1097 GGGGCCT [GA]G^G[ACGT]CC[CT]
FbaI 870 TGATCA T^GATCA
FokI 168 CATCC GGATG(9/13)
HapII 191 CCGG C^CGG
HhaI 1053 GCGC GCG^C
HinP1I 1053 GCGC G^CGC
HinfI 183 GAATC G^A[ACGT]TC
HpaII 191 CCGG C^CGG
MluNI 485 TGGCCA TGG^CCA
MscI 485 TGGCCA TGG^CCA
MslI 282 CACCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 191 CCGG C^CGG
MvnI 1128 CGCG CG^CG
RcaI 873 TCATGA T^CATGA
SapI 981 GCTCTTC GCTCTTC(1/4)
SpeI 52 ACTAGT A^CTAGT
SspBI 548 TGTACA T^GTACA
TfiI 183 GAATC G^A[AT]TC
XcmI 473 CCAAGACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AciI 465 CCGC CCGC(-3/-1)
1129 GCGG
AcsI 781 AAATTC [GA]^AATT[CT]
1072 AAATTT
AlwI 558 GATCC GGATC(4/5)
587 GATCC
ApoI 781 AAATTC [GA]^AATT[CT]
1072 AAATTT
BanII 97 GAGCCC G[GA]GC[CT]^C
400 GGGCCC
BbvCI 86 CCTCAGC CCTCAGC(-5/-2)
963 GCTGAGG
BbvI 270 GCAGC GCAGC(8/12)
978 GCTGC
BsaI 311 GAGACC GGTCTC(1/5)
991 GAGACC
BseRI 300 CTCCTC GAGGAG(10/8)
492 CTCCTC
BsrDI 801 CATTGC GCAATG(2/0)
520 GCAATG
BstYI 557 AGATCC [GA]^GATC[CT]
586 AGATCC
Cac8I 483 GCTGGC GC[ACGT]^[ACGT]GC
690 GCAGGC
EaeI 188 TGGCCG [CT]^GGCC[GA]
485 TGGCCA
EarI 982 CTCTTC CTCTTC(1/4)
1110 CTCTTC
EcoT14I 101 CCTTGG C^C[AT][AT]GG
530 CCTTGG
Fnu4HI 270 GCAGC GC^[ACGT]GC
978 GCTGC
ItaI 270 GCAGC GC^[ACGT]GC
978 GCTGC
Ksp632I 982 CTCTTC CTCTTC(1/4)
1110 CTCTTC
MflI 557 AGATCC [GA]^GATC[CT]
586 AGATCC
MseI 770 TTAA T^TAA
1139 TTAA
MspA1I 44 CAGCTG C[AC]G^C[GT]G
961 CAGCTG
PvuII 44 CAGCTG CAG^CTG
961 CAGCTG
ScaI 120 AGTACT AGT^ACT
673 AGTACT
StyI 101 CCTTGG C^C[AT][AT]GG
530 CCTTGG
Tru9I 770 TTAA T^TAA
1139 TTAA
TseI 270 GCAGC G^C[AT]GC
978 GCTGC
Tsp45I 139 GTGAC ^GT[GC]AC
856 GTCAC
XhoII 557 AGATCC [GA]^GATC[CT]
586 AGATCC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AfaI AluI BfaI BmyI BsaJI
BsiYI BslI BsmAI Bsp1286I BsrI
BssKI BstNI Cfr13I DdeI DpnII
EcoRII HaeIII HphI MaeI MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI Tsp509I
TspRI