Restriction map for
pFN21AE1094
##################################
# NO CUT ENZYMES ( 135 enzymes) #
##################################
AatII Acc65I AccII AccIII AflII
AgeI AhdI Alw44I AocI Aor51HI
ApaI ApaLI AscI Asp700I Asp718I
AspEI AspI AvaI AvaII AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BcgI BciVI BcnI
BfrI BlnI BpuAI BsaI BsaWI
BseAI BsiEI BsiWI BsmBI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BstBI
BstEII BstPI BstUI BstXI Bsu36I
CfoI Cfr10I ClaI CpoI DraII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO109I EcoO65I EcoRI
EcoRV FauI FseI FspI HaeII
HapII HhaI HinP1I HpaII KasI
KpnI KspI MluI MroI MspA1I
MspI MvnI NaeI NarI NciI
NgoMIV NlaIV NotI NruI NspI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PstI PvuI PvuII RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 53 enzymes) #
###################################
AccI 1639 GTATAC GT^[AC][GT]AC
AciI 1881 GCGG CCGC(-3/-1)
AclI 1951 AACGTT AA^CGTT
AcyI 2441 GACGCC G[GA]^CG[CT]C
AflIII 2087 ACACGT A^C[GA][CT]GT
AlwNI 2105 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 2476 ATTAAT AT^TAAT
AsnI 2476 ATTAAT AT^TAAT
BalI 1565 TGGCCA TGG^CCA
BglI 2166 GCCTGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 657 AGATCT A^GATCT
BlpI 268 GCTAAGC GC^T[ACGT]AGC
Bpu1102I 268 GCTAAGC GC^T[ACGT]AGC
BsaAI 2088 CACGTA [CT]AC^GT[GA]
BsaBI 1069 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 2441 GACGCC G[GA]^CG[CT]C
BseRI 2423 CTCCTC GAGGAG(10/8)
BsmFI 2503 GGGAC GGGAC(10/14)
BsmI 952 GCATTC GAATGC(1/-1)
BspHI 147 TCATGA T^CATGA
BssSI 1511 CTCGTG CACGAG(-5/-1)
Bst1107I 1639 GTATAC GTA^TAC
BstAPI 376 GCAATGATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstYI 657 AGATCT [GA]^GATC[CT]
BstZ17I 1639 GTATAC GTA^TAC
CelII 268 GCTAAGC GC^T[ACGT]AGC
Cfr13I 2080 GGCCC G^G[ACGT]CC
DraIII 1424 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 2209 CCATGG C^C[GA][CT]GG
EaeI 1565 TGGCCA [CT]^GGCC[GA]
HgaI 2441 GACGC GACGC(5/10)
Hin1I 2441 GACGCC G[GA]^CG[CT]C
HincII 1954 GTTAAC GT[CT]^[GA]AC
HindII 1954 GTTAAC GT[CT]^[GA]AC
HpaI 1954 GTTAAC GTT^AAC
MamI 1069 GATTCTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 461 CAATTG C^AATTG
MflI 657 AGATCT [GA]^GATC[CT]
MluNI 1565 TGGCCA TGG^CCA
MscI 1565 TGGCCA TGG^CCA
MunI 461 CAATTG C^AATTG
NcoI 2209 CCATGG C^CATGG
NdeI 2233 CATATG CA^TATG
NheI 747 GCTAGC G^CTAGC
PflMI 1568 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2476 ATTAAT AT^TAAT
Psp1406I 1951 AACGTT AA^CGTT
RcaI 147 TCATGA T^CATGA
SapI 154 GCTCTTC GCTCTTC(1/4)
Sau96I 2080 GGCCC G^G[ACGT]CC
SmlI 541 CTTGAG C^T[CT][GA]AG
Van91I 1568 CCAAGTATTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 657 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AlwI 688 GATCC GGATC(4/5)
1420 GGATC
AspHI 769 GAGCAC G[AT]GC[AT]^C
1924 GAGCAC
BbvCI 217 CCTCAGC CCTCAGC(-5/-2)
2569 GCTGAGG
BclI 566 TGATCA T^GATCA
897 TGATCA
BpmI 365 CTGGAG CTGGAG(16/14)
2032 CTGGAG
BsiHKAI 769 GAGCAC G[AT]GC[AT]^C
1924 GAGCAC
BsrDI 199 GCAATG GCAATG(2/0)
376 GCAATG
BstF5I 563 GGATG GGATG(2/0)
1377 GGATG
EarI 155 CTCTTC CTCTTC(1/4)
1276 GAAGAG
EcoNI 691 CCTTCTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
916 CCTACTGAAGG
EcoT14I 2209 CCATGG C^C[AT][AT]GG
2525 CCTTGG
EcoT22I 950 ATGCAT ATGCA^T
1247 ATGCAT
FbaI 566 TGATCA T^GATCA
897 TGATCA
FokI 563 GGATG GGATG(9/13)
1377 GGATG
Ksp632I 155 CTCTTC CTCTTC(1/4)
1276 GAAGAG
NsiI 950 ATGCAT ATGCA^T
1247 ATGCAT
StyI 2209 CCATGG C^C[AT][AT]GG
2525 CCTTGG
TaqI 876 TCGA T^CGA
2439 TCGA
Tsp45I 178 GTCAC ^GT[GC]AC
649 GTCAC
TthHB8I 876 TCGA T^CGA
2439 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AcsI AfaI AluI ApoI BbvI
BfaI BmyI BsaJI BsgI BsiYI
BslI BsmAI Bsp1286I BsrI BssKI
BstNI Cac8I DdeI DpnII DraI
EcoRII Fnu4HI HaeIII HindIII HinfI
HphI ItaI MaeI MaeII MaeIII
MboI MboII MnlI MseI MslI
MvaI MwoI NdeII NlaIII PleI
RsaI Sau3AI ScrFI SfaNI SfcI
TfiI Tru9I TseI Tsp509I TspRI