Restriction map for
pFN21AE6347
##################################
# NO CUT ENZYMES ( 128 enzymes) #
##################################
Acc65I AccI AccIII AclI AcsI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaLI ApoI AscI
AseI AsnI Asp718I AspEI AspI
AviII AvrII BamHI BbrPI BbvCI
BcgI BfrI BglI BglII BlnI
BsaAI BsaBI BsaWI BseAI BsgI
BsiEI BsiWI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstYI BstZ17I
Bsu36I ClaI CpoI DraI DsaI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FauI FseI FspI HincII HindII
HpaI HphI KpnI KspI MamI
MfeI MflI MluI MroI MslI
MunI NcoI NdeI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
ScaI SfiI SfuI SmlI SnaBI
SpeI Sse8387I SspBI SspI StuI
SwaI Tsp45I Tth111I Van91I XbaI
XcmI XhoI XhoII
###################################
# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AatII 121 GACGTC GACGT^C
AfaI 63 GTAC GT^AC
AlwI 378 GATCC GGATC(4/5)
ApaI 587 GGGCCC GGGCC^C
Asp700I 552 GAATCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspHI 455 GTGCTC G[AT]GC[AT]^C
BalI 320 TGGCCA TGG^CCA
BbeI 153 GGCGCC GGCGC^C
BbsI 788 GTCTTC GAAGAC(2/6)
BciVI 187 GGATAC GTATCC(6/5)
BclI 737 TGATCA T^GATCA
BlpI 466 GCTGAGC GC^T[ACGT]AGC
BpmI 140 CTGGAG CTGGAG(16/14)
Bpu1102I 466 GCTGAGC GC^T[ACGT]AGC
BpuAI 788 GTCTTC GAAGAC(2/6)
BsaI 143 GAGACC GGTCTC(1/5)
BsiHKAI 455 GTGCTC G[AT]GC[AT]^C
BsmAI 143 GAGAC GTCTC(1/5)
BsmI 753 GAATGC GAATGC(1/-1)
BspHI 858 TCATGA T^CATGA
BsrBI 67 GAGCGG CCGCTC(-3/-3)
BsrFI 605 GCCGGC [GA]^CCGG[CT]
CelII 466 GCTGAGC GC^T[ACGT]AGC
Cfr10I 605 GCCGGC [GA]^CCGG[CT]
DraIII 634 CACCTGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 779 GACAGGCTTGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 636 CCTGGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 758 CCAAGG C^C[AT][AT]GG
FbaI 737 TGATCA T^GATCA
HaeII 153 GGCGCC [GA]GCGC^[CT]
HgaI 43 GACGC GACGC(5/10)
HindIII 652 AAGCTT A^AGCTT
KasI 153 GGCGCC G^GCGCC
MaeII 122 ACGT A^CGT
MaeIII 896 GTTAC ^GT[ACGT]AC
MluNI 320 TGGCCA TGG^CCA
MscI 320 TGGCCA TGG^CCA
MspA1I 464 CAGCTG C[AC]G^C[GT]G
NaeI 605 GCCGGC GCC^GGC
NarI 153 GGCGCC GG^CGCC
NgoMIV 605 GCCGGC G^CCGGC
NheI 889 GCTAGC G^CTAGC
PpuMI 497 AGGTCCT [GA]G^G[AT]CC[CT]
PvuII 464 CAGCTG CAG^CTG
RcaI 858 TCATGA T^CATGA
RsaI 63 GTAC GT^AC
SapI 625 GAAGAGC GCTCTTC(1/4)
SexAI 635 ACCTGGT A^CC[AT]GGT
SfcI 965 CTACAG C^T[GA][CT]AG
SfoI 153 GGCGCC GGC^GCC
SgrAI 604 CGCCGGCG C[GA]^CCGG[CT]G
SmaI 815 CCCGGG CCC^GGG
SphI 302 GCATGC GCATG^C
StyI 758 CCAAGG C^C[AT][AT]GG
XmaCI 815 CCCGGG C^CCGGG
XmaI 815 CCCGGG C^CCGGG
XmnI 552 GAATCGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 23 enzymes) #
###################################
AccII 12 CGCG CG^CG
945 CGCG
AlwNI 55 CAGTCCCTG CAG[ACGT][ACGT][ACGT]^CTG
631 CAGCACCTG
AvaII 102 GGACC G^G[AT]CC
498 GGTCC
BanI 153 GGCGCC G^G[CT][GA]CC
932 GGCACC
BanII 587 GGGCCC G[GA]GC[CT]^C
833 GAGCCC
BseRI 239 CTCCTC GAGGAG(10/8)
311 GAGGAG
BsrI 885 ACTGG ACTGG(1/-1)
54 CCAGT
BstF5I 126 CATCC GGATG(2/0)
83 GGATG
BstUI 12 CGCG CG^CG
945 CGCG
EaeI 51 CGGCCA [CT]^GGCC[GA]
320 TGGCCA
EarI 766 CTCTTC CTCTTC(1/4)
625 GAAGAG
FokI 126 CATCC GGATG(9/13)
83 GGATG
Ksp632I 766 CTCTTC CTCTTC(1/4)
625 GAAGAG
MseI 16 TTAA T^TAA
73 TTAA
MvnI 12 CGCG CG^CG
945 CGCG
NspI 302 GCATGC [GA]CATG^[CT]
938 GCATGT
PleI 26 GAGTC GAGTC(4/5)
439 GAGTC
TaqI 215 TCGA T^CGA
831 TCGA
TfiI 217 GATTC G^A[AT]TC
552 GAATC
Tru9I 16 TTAA T^TAA
73 TTAA
Tsp509I 223 AATT ^AATT
482 AATT
TspRI 272 CACTG CA[GC]TG[ACGT][ACGT]^
393 CACTG
TthHB8I 215 TCGA T^CGA
831 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 50 enzymes) #
#########################################
AciI AcyI AluI AvaI BbvI
BcnI BfaI BmyI BsaHI BsaJI
BsiYI BslI BsmFI BsoBI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoO109I EcoRII
Fnu4HI HaeIII HapII HhaI Hin1I
HinP1I HinfI HpaII ItaI MaeI
MboI MboII MnlI MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
Sau3AI Sau96I ScrFI SfaNI TseI