Restriction map for
pFN21AE5206
##################################
# NO CUT ENZYMES ( 142 enzymes) #
##################################
AccI AccII AccIII AclI AcsI
AflII AflIII AgeI Alw44I AlwNI
AocI Aor51HI ApaI ApaLI ApoI
AscI AseI AsnI Asp700I AspI
AviII AvrII BamHI BbeI BbrPI
BcgI BciVI BclI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI Bsp1407I BspEI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstZ17I Bsu36I
CelII Cfr10I CpoI DraI DraIII
DrdI DsaI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoT22I
FbaI FseI FspI HincII HindII
HindIII HpaI KasI KspI MamI
MfeI MluI MroI MseI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PmaCI PmeI PmlI
PshBI Psp1406I PstI PvuI RsrII
SacII SalI SapI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI Tru9I
Tth111I Van91I XbaI XhoI XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 51 enzymes) #
###################################
AatII 250 GACGTC GACGT^C
Acc65I 647 GGTACC G^GTACC
AhdI 502 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Asp718I 647 GGTACC G^GTACC
AspEI 502 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 49 GAGCTC G[AT]GC[AT]^C
AvaI 40 CCCGAG C^[CT]CG[GA]G
BalI 593 TGGCCA TGG^CCA
BanI 647 GGTACC G^G[CT][GA]CC
BanII 49 GAGCTC G[GA]GC[CT]^C
BbvCI 741 CCTCAGC CCTCAGC(-5/-2)
BfaI 196 CTAG C^TAG
BmyI 49 GAGCTC G[AGT]GC[ACT]^C
BpmI 274 CTGGAG CTGGAG(16/14)
BsaI 280 GAGACC GGTCTC(1/5)
BsiHKAI 49 GAGCTC G[AT]GC[AT]^C
BsmAI 280 GAGAC GTCTC(1/5)
BsoBI 40 CCCGAG C^[CT]CG[GA]G
Bsp1286I 49 GAGCTC G[AGT]GC[ACT]^C
BspDI 70 ATCGAT AT^CGAT
BspHI 116 TCATGA T^CATGA
BspMI 185 ACCTGC ACCTGC(4/8)
BsrI 309 ACTGG ACTGG(1/-1)
BstYI 162 GGATCT [GA]^GATC[CT]
ClaI 70 ATCGAT AT^CGAT
EaeI 593 TGGCCA [CT]^GGCC[GA]
Eam1105I 502 GACAAGGGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EarI 112 CTCTTC CTCTTC(1/4)
EcoNI 420 CCTAACCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRV 616 GATATC GAT^ATC
EcoT14I 425 CCAAGG C^C[AT][AT]GG
HaeII 107 AGCGCC [GA]GCGC^[CT]
HgaI 481 GACGC GACGC(5/10)
KpnI 647 GGTACC GGTAC^C
Ksp632I 112 CTCTTC CTCTTC(1/4)
MaeI 196 CTAG C^TAG
MaeIII 151 GTCAC ^GT[ACGT]AC
MflI 162 GGATCT [GA]^GATC[CT]
MluNI 593 TGGCCA TGG^CCA
MscI 593 TGGCCA TGG^CCA
MslI 153 CACCACTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MwoI 724 GCTGATGTCGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PshAI 246 GACAGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 116 TCATGA T^CATGA
SacI 49 GAGCTC GAGCT^C
SmlI 652 CTCAAG C^T[CT][GA]AG
StyI 425 CCAAGG C^C[AT][AT]GG
Tsp45I 151 GTCAC ^GT[GC]AC
Tsp509I 440 AATT ^AATT
XcmI 569 CCAAGCTCCATGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 162 GGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcyI 250 GACGTC G[GA]^CG[CT]C
481 GACGCC
AfaI 300 GTAC GT^AC
648 GTAC
AvaII 36 GGACC G^G[AT]CC
509 GGTCC
BbsI 243 GAAGAC GAAGAC(2/6)
499 GAAGAC
BpuAI 243 GAAGAC GAAGAC(2/6)
499 GAAGAC
BsaHI 250 GACGTC G[GA]^CG[CT]C
481 GACGCC
BsgI 187 CTGCAC GTGCAG(16/14)
692 GTGCAG
CfoI 108 GCGC GCG^C
516 GCGC
Cfr13I 36 GGACC G^G[ACGT]CC
509 GGTCC
DraII 35 GGGACCC [GA]G^G[ACGT]CC[CT]
508 GGGTCCC
EcoO109I 35 GGGACCC [GA]G^G[ACGT]CC[CT]
508 GGGTCCC
FauI 213 CCCGC CCCGC(4/6)
340 GCGGG
HhaI 108 GCGC GCG^C
516 GCGC
Hin1I 250 GACGTC G[GA]^CG[CT]C
481 GACGCC
HinP1I 108 GCGC G^CGC
516 GCGC
HphI 152 TCACC GGTGA(8/7)
680 TCACC
MaeII 239 ACGT A^CGT
251 ACGT
NlaIII 117 CATG CATG^
577 CATG
PleI 7 GACTC GAGTC(4/5)
137 GAGTC
PpuMI 35 GGGACCC [GA]G^G[AT]CC[CT]
508 GGGTCCC
PvuII 179 CAGCTG CAG^CTG
695 CAGCTG
RsaI 300 GTAC GT^AC
648 GTAC
Sau96I 36 GGACC G^G[ACGT]CC
509 GGTCC
SfaNI 83 GCATC GCATC(5/9)
344 GCATC
StuI 417 AGGCCT AGG^CCT
636 AGGCCT
TfiI 68 GAATC G^A[AT]TC
328 GAATC
TspRI 156 CACTG CA[GC]TG[ACGT][ACGT]^
702 CAGTG
#########################################
# 3 OR MORE CUT ENZYMES ( 38 enzymes) #
#########################################
AciI AluI AlwI BbvI BcnI
BsaJI BseRI BsiYI BslI BsmFI
BssKI BstF5I BstNI Cac8I DdeI
DpnII EcoRII Fnu4HI FokI HaeIII
HapII HinfI HpaII ItaI MboI
MboII MnlI MspA1I MspI MvaI
NciI NdeII NlaIV Sau3AI ScrFI
TaqI TseI TthHB8I