Restriction map for
pFN21AE5215
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# NO CUT ENZYMES ( 167 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AflIII AgeI AhdI Alw44I
AlwI AlwNI AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII AvrII BalI BamHI BbrPI
BbsI BcgI BciVI BclI BcnI
BfaI BfrI BglI BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaI
BsaJI BsaWI BseAI BseRI BsiHKAI
BsiWI BsmBI BsmI BsoBI Bsp1407I
BspDI BspEI BspHI BspLU11I BspMI
BsrBI BsrDI BsrFI BsrGI Bst1107I
BstAPI BstBI BstEII BstPI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraII DraIII DrdI DsaI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO109I EcoO65I EcoRI
EcoRV EcoT14I EcoT22I FbaI FseI
FspI HaeIII HpaI KpnI KspI
MaeI MaeII MamI MfeI MluI
MluNI MroI MscI MunI NaeI
NciI NcoI NdeI NgoMIV NheI
NotI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsaI RsrII SacI SacII SapI
SbfI ScaI SfcI SfiI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
StyI SwaI TfiI Tsp45I Tth111I
Van91I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 573 GTCGAC GT^[AC][GT]AC
AcsI 276 AAATTT [GA]^AATT[CT]
AcyI 665 GGCGCC G[GA]^CG[CT]C
ApoI 276 AAATTT [GA]^AATT[CT]
AvaII 535 GGACC G^G[AT]CC
BanI 665 GGCGCC G^G[CT][GA]CC
BanII 697 GAGCCC G[GA]GC[CT]^C
BbeI 665 GGCGCC GGCGC^C
BbvCI 3 GCTGAGG CCTCAGC(-5/-2)
BglII 42 AGATCT A^GATCT
BmyI 697 GAGCCC G[AGT]GC[ACT]^C
BpmI 446 CTCCAG CTGGAG(16/14)
BsaHI 665 GGCGCC G[GA]^CG[CT]C
BsgI 370 GTGCAG GTGCAG(16/14)
BsiEI 680 CGACCG CG[GA][CT]^CG
BsmAI 628 GAGAC GTCTC(1/5)
Bsp1286I 697 GAGCCC G[AGT]GC[ACT]^C
BssHII 716 GCGCGC G^CGCGC
BssSI 182 CACGAG CACGAG(-5/-1)
BstF5I 108 GGATG GGATG(2/0)
BstYI 42 AGATCT [GA]^GATC[CT]
Cfr13I 535 GGACC G^G[ACGT]CC
DdeI 4 CTGAG C^T[ACGT]AG
DraI 250 TTTAAA TTT^AAA
FokI 108 GGATG GGATG(9/13)
HaeII 665 GGCGCC [GA]GCGC^[CT]
HapII 380 CCGG C^CGG
HgaI 158 GCGTC GACGC(5/10)
Hin1I 665 GGCGCC G[GA]^CG[CT]C
HincII 573 GTCGAC GT[CT]^[GA]AC
HindII 573 GTCGAC GT[CT]^[GA]AC
HindIII 255 AAGCTT A^AGCTT
HpaII 380 CCGG C^CGG
KasI 665 GGCGCC G^GCGCC
MaeIII 235 GTTAC ^GT[ACGT]AC
MflI 42 AGATCT [GA]^GATC[CT]
MslI 595 CATGTCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 244 CAGCTG C[AC]G^C[GT]G
MspI 380 CCGG C^CGG
NarI 665 GGCGCC GG^CGCC
PvuII 244 CAGCTG CAG^CTG
SalI 573 GTCGAC G^TCGAC
Sau96I 535 GGACC G^G[ACGT]CC
SexAI 230 ACCAGGT A^CC[AT]GGT
SfoI 665 GGCGCC GGC^GCC
TaqI 574 TCGA T^CGA
TthHB8I 574 TCGA T^CGA
XhoII 42 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
BsrI 36 CCAGT ACTGG(1/-1)
460 CCAGT
BssKI 231 CCAGG ^CC[ACGT]GG
538 CCAGG
BstNI 231 CCAGG CC^[AT]GG
538 CCAGG
BstXI 460 CCAGTGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
581 CCAGCGTGGTGG
Cac8I 151 GCTTGC GC[ACGT]^[ACGT]GC
716 GCGCGC
DpnII 43 GATC ^GATC
130 GATC
EcoRII 231 CCAGG ^CC[AT]GG
538 CCAGG
FauI 390 GCGGG CCCGC(4/6)
562 GCGGG
HinfI 266 GAGTC G^A[ACGT]TC
571 GAGTC
HphI 88 GGTGA GGTGA(8/7)
215 TCACC
MboI 43 GATC ^GATC
130 GATC
MseI 124 TTAA T^TAA
251 TTAA
MvaI 231 CCAGG CC^[AT]GG
538 CCAGG
NdeII 43 GATC ^GATC
130 GATC
NlaIII 163 CATG CATG^
595 CATG
NlaIV 534 GGGACC GG[ACGT]^[ACGT]CC
665 GGCGCC
PleI 266 GAGTC GAGTC(4/5)
571 GAGTC
Sau3AI 43 GATC ^GATC
130 GATC
ScrFI 231 CCAGG CC^[ACGT]GG
538 CCAGG
SfaNI 484 GATGC GCATC(5/9)
489 GATGC
Tru9I 124 TTAA T^TAA
251 TTAA
#########################################
# 3 OR MORE CUT ENZYMES ( 22 enzymes) #
#########################################
AccII AciI AluI BbvI BsiYI
BslI BsmFI BstUI CfoI EarI
Fnu4HI HhaI HinP1I ItaI Ksp632I
MboII MnlI MvnI MwoI TseI
Tsp509I TspRI