Restriction map for
pFN21AE6251
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# NO CUT ENZYMES ( 173 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AfaI AflII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaLI AscI AseI AsnI Asp700I
Asp718I AspEI AspHI AvaI AvaII
AviII AvrII BalI BamHI BanI
BbeI BbrPI BbvCI BciVI BclI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaAI BsaBI BsaHI
BsaI BsaJI BsaWI BseAI BseRI
BsiEI BsiHKAI BsiWI BsiYI BslI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI Bst1107I BstBI
BstEII BstPI BstUI BstZ17I Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraIII DrdI DsaI EaeI
EagI Eam1105I EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoT14I EcoT22I FbaI FseI FspI
HaeII HapII HgaI Hin1I HpaII
KasI KpnI Ksp632I KspI MaeI
MamI MfeI MluI MluNI MroI
MscI MspA1I MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflMI PinAI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RsaI RsrII SacI SacII SalI
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StyI SwaI Van91I XbaI XhoI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 301 AAATTT [GA]^AATT[CT]
AflIII 390 ACATGT A^C[GA][CT]GT
AluI 362 AGCT AG^CT
ApaI 645 GGGCCC GGGCC^C
ApoI 301 AAATTT [GA]^AATT[CT]
AspI 586 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanII 645 GGGCCC G[GA]GC[CT]^C
BbsI 667 GTCTTC GAAGAC(2/6)
BbvI 193 GCAGC GCAGC(8/12)
BcgI 532 CGAAAACTTTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BglII 430 AGATCT A^GATCT
BmyI 645 GGGCCC G[AGT]GC[ACT]^C
BpuAI 667 GTCTTC GAAGAC(2/6)
BsgI 596 GTGCAG GTGCAG(16/14)
BsmFI 775 GTCCC GGGAC(10/14)
Bsp1286I 645 GGGCCC G[AGT]GC[ACT]^C
BspHI 693 TCATGA T^CATGA
BspLU11I 390 ACATGT A^CATGT
BstAPI 598 GCAGGAGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstF5I 129 CATCC GGATG(2/0)
BstXI 581 CCAAAGACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 248 GCGC GCG^C
EcoRV 346 GATATC GAT^ATC
FauI 777 CCCGC CCCGC(4/6)
FokI 129 CATCC GGATG(9/13)
HhaI 248 GCGC GCG^C
HinP1I 248 GCGC G^CGC
HincII 735 GTTAAC GT[CT]^[GA]AC
HindII 735 GTTAAC GT[CT]^[GA]AC
HindIII 361 AAGCTT A^AGCTT
HpaI 735 GTTAAC GTT^AAC
MaeII 149 ACGT A^CGT
NspI 390 ACATGT [GA]CATG^[CT]
PflFI 586 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
RcaI 693 TCATGA T^CATGA
SfcI 677 CTATAG C^T[GA][CT]AG
SmlI 315 CTCAAG C^T[CT][GA]AG
StuI 272 AGGCCT AGG^CCT
TaqI 531 TCGA T^CGA
TseI 193 GCAGC G^C[AT]GC
Tth111I 586 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 531 TCGA T^CGA
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# TWO CUT ENZYMES ( 20 enzymes) #
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BpmI 289 CTGGAG CTGGAG(16/14)
686 CTGGAG
BsmAI 228 GTCTC GTCTC(1/5)
682 GTCTC
BssKI 110 CCAGG ^CC[ACGT]GG
718 CCTGG
BstNI 110 CCAGG CC^[AT]GG
718 CCTGG
Cfr13I 645 GGGCC G^G[ACGT]CC
646 GGCCC
DraII 644 GGGGCCC [GA]G^G[ACGT]CC[CT]
645 GGGCCCC
EcoO109I 644 GGGGCCC [GA]G^G[ACGT]CC[CT]
645 GGGCCCC
EcoRII 110 CCAGG ^CC[AT]GG
718 CCTGG
Fnu4HI 193 GCAGC GC^[ACGT]GC
373 GCCGC
ItaI 193 GCAGC GC^[ACGT]GC
373 GCCGC
MboII 13 TCTTC GAAGA(8/7)
668 TCTTC
MslI 148 CACGTTTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
694 CATGAATGTG
MvaI 110 CCAGG CC^[AT]GG
718 CCTGG
MwoI 354 GCTGGTTAAGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
598 GCAGGAGATGC
PleI 54 GACTC GAGTC(4/5)
637 GACTC
Sau96I 645 GGGCC G^G[ACGT]CC
646 GGCCC
ScrFI 110 CCAGG CC^[ACGT]GG
718 CCTGG
SfaNI 399 GATGC GCATC(5/9)
604 GATGC
Tsp509I 78 AATT ^AATT
302 AATT
XcmI 110 CCAGGCCATTTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
278 CCAAACAAACACTGG
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# 3 OR MORE CUT ENZYMES ( 23 enzymes) #
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AciI AlwI BsrI BstYI DdeI
DpnII HaeIII HinfI HphI MaeIII
MboI MflI MnlI MseI NdeII
NlaIII NlaIV Sau3AI TfiI Tru9I
Tsp45I TspRI XhoII