Restriction map for
pFN21AE6213
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# NO CUT ENZYMES ( 179 enzymes) #
##################################
AatII Acc65I AccI AccII AclI
AcyI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AvaI AvaII AviII
BalI BamHI BanI BanII BbeI
BcgI BciVI BclI BcnI BfrI
BglI BglII BlpI BmyI BpmI
Bpu1102I BsaBI BsaHI BsaI BseRI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmFI BsoBI Bsp1286I Bsp1407I BspDI
BspHI BspLU11I BspMI BsrBI BsrDI
BsrFI BsrGI BsrI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstXI BstZ17I Bsu36I CelII
CfoI Cfr10I Cfr13I ClaI CpoI
DraI DraII DraIII DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FbaI FseI
FspI HaeII HaeIII HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeIII MamI
MfeI MluI MluNI MscI MspA1I
MunI MvnI MwoI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PmeI
PpuMI PshAI PshBI Psp1406I PstI
PvuI PvuII RcaI RsrII SacI
SacII SalI SapI Sau96I SbfI
SexAI SfaNI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI Tsp45I TspRI Tth111I XbaI
XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccIII 6 TCCGGA T^CCGGA
AciI 592 CCGC CCGC(-3/-1)
AluI 588 AGCT AG^CT
AlwNI 286 CAGGGTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 430 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvrII 52 CCTAGG C^CTAGG
BbrPI 268 CACGTG CAC^GTG
BbsI 382 GAAGAC GAAGAC(2/6)
BbvCI 177 GCTGAGG CCTCAGC(-5/-2)
BbvI 344 GCAGC GCAGC(8/12)
BfaI 53 CTAG C^TAG
BlnI 52 CCTAGG C^CTAGG
BpuAI 382 GAAGAC GAAGAC(2/6)
BsaAI 268 CACGTG [CT]AC^GT[GA]
BsaWI 6 TCCGGA [AT]^CCGG[AT]
BseAI 6 TCCGGA T^CCGGA
BsmAI 186 GAGAC GTCTC(1/5)
BspEI 6 TCCGGA T^CCGGA
BssKI 285 CCAGG ^CC[ACGT]GG
BstNI 285 CCAGG CC^[AT]GG
BstYI 48 AGATCC [GA]^GATC[CT]
Cac8I 263 GCCAGC GC[ACGT]^[ACGT]GC
EarI 355 CTCTTC CTCTTC(1/4)
EcoRII 285 CCAGG ^CC[AT]GG
EcoT14I 52 CCTAGG C^C[AT][AT]GG
Fnu4HI 344 GCAGC GC^[ACGT]GC
HapII 7 CCGG C^CGG
HgaI 279 GCGTC GACGC(5/10)
HpaII 7 CCGG C^CGG
HphI 351 TCACC GGTGA(8/7)
ItaI 344 GCAGC GC^[ACGT]GC
Ksp632I 355 CTCTTC CTCTTC(1/4)
MaeI 53 CTAG C^TAG
MflI 48 AGATCC [GA]^GATC[CT]
MroI 6 TCCGGA T^CCGGA
MseI 451 TTAA T^TAA
MspI 7 CCGG C^CGG
MvaI 285 CCAGG CC^[AT]GG
NspI 119 GCATGT [GA]CATG^[CT]
PleI 188 GACTC GAGTC(4/5)
PmaCI 268 CACGTG CAC^GTG
PmlI 268 CACGTG CAC^GTG
ScaI 416 AGTACT AGT^ACT
ScrFI 285 CCAGG CC^[ACGT]GG
SfcI 316 CTACAG C^T[GA][CT]AG
StyI 52 CCTAGG C^C[AT][AT]GG
TaqI 45 TCGA T^CGA
Tru9I 451 TTAA T^TAA
TseI 344 GCAGC G^C[AT]GC
TthHB8I 45 TCGA T^CGA
XhoII 48 AGATCC [GA]^GATC[CT]
XmnI 430 GAAGCAATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
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AlwI 49 GATCC GGATC(4/5)
273 GGATC
BsaJI 52 CCTAGG C^C[ACGT][ACGT]GG
285 CCAGGG
BsiYI 264 CCAGCACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
285 CCAGGGTCTGG
BslI 264 CCAGCACGTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
285 CCAGGGTCTGG
BsmI 321 GAATGC GAATGC(1/-1)
78 GCATTC
DpnII 49 GATC ^GATC
274 GATC
EcoRI 166 GAATTC G^AATTC
375 GAATTC
MaeII 269 ACGT A^CGT
462 ACGT
MboI 49 GATC ^GATC
274 GATC
MslI 544 CACAGTAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
566 CACATGGATG
NdeII 49 GATC ^GATC
274 GATC
PflMI 264 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
285 CCAGGGTCTGG
Sau3AI 49 GATC ^GATC
274 GATC
Van91I 264 CCAGCACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
285 CCAGGGTCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 13 enzymes) #
#########################################
AcsI AfaI ApoI BstF5I DdeI
FokI HinfI MboII MnlI NlaIII
RsaI TfiI Tsp509I