Restriction map for
pFN21AE1553
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AvrII BalI BamHI BbeI
BbrPI BbvCI BciVI BclI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BsaHI BsaI BsaWI BseAI
BsiHKAI BsiWI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrGI BssHII BssSI
Bst1107I BstAPI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DrdI Eam1105I Eco47III Eco81I
EcoNI EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI Hin1I HpaI KasI
KpnI KspI MfeI MluI MluNI
MroI MscI MslI MunI MvnI
NarI NcoI NdeI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmaCI PmeI PmlI PpuMI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI SexAI SfiI
SfoI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 42 enzymes) #
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AcsI 459 AAATTC [GA]^AATT[CT]
ApoI 459 AAATTC [GA]^AATT[CT]
Asp700I 484 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 968 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 905 TGCGCA TGC^GCA
BanI 100 GGTGCC G^G[CT][GA]CC
BbsI 1507 GAAGAC GAAGAC(2/6)
BcgI 1268 GCATGACCGTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BpuAI 1507 GAAGAC GAAGAC(2/6)
BsaAI 262 TACGTG [CT]AC^GT[GA]
BsaBI 699 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiEI 657 CGGCCG CG[GA][CT]^CG
BspHI 1221 TCATGA T^CATGA
BspMI 68 ACCTGC ACCTGC(4/8)
BsrBI 417 GAGCGG CCGCTC(-3/-3)
BstBI 404 TTCGAA TT^CGAA
DdeI 34 CTCAG C^T[ACGT]AG
DraI 373 TTTAAA TTT^AAA
DraII 815 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 1556 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 657 CGGCCG C^GGCCG
EarI 132 GAAGAG CTCTTC(1/4)
EclXI 657 CGGCCG C^GGCCG
Eco52I 657 CGGCCG C^GGCCG
EcoO109I 815 AGGCCCC [GA]G^G[ACGT]CC[CT]
FspI 905 TGCGCA TGC^GCA
HgaI 735 GCGTC GACGC(5/10)
HincII 691 GTCAAC GT[CT]^[GA]AC
HindII 691 GTCAAC GT[CT]^[GA]AC
HindIII 1475 AAGCTT A^AGCTT
Ksp632I 132 GAAGAG CTCTTC(1/4)
MamI 699 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 1815 ACATGC [GA]CATG^[CT]
NspV 404 TTCGAA TT^CGAA
PflFI 968 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1410 GACAGAGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 1221 TCATGA T^CATGA
ScaI 1467 AGTACT AGT^ACT
SfuI 404 TTCGAA TT^CGAA
SmlI 979 CTTGAG C^T[CT][GA]AG
Tth111I 968 GACATGGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmnI 484 GAAGTCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AflIII 263 ACGTGT A^C[GA][CT]GT
1074 ACGTGT
AlwI 350 GATCC GGATC(4/5)
553 GATCC
AlwNI 757 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
1560 CAGTGACTG
AvaI 836 CCCGAG C^[CT]CG[GA]G
877 CCCGAG
BfaI 247 CTAG C^TAG
329 CTAG
BsgI 70 CTGCAC GTGCAG(16/14)
425 GTGCAG
BsmBI 736 CGTCTC CGTCTC(1/5)
653 GAGACG
BsoBI 836 CCCGAG C^[CT]CG[GA]G
877 CCCGAG
BsrDI 710 GCAATG GCAATG(2/0)
1148 GCAATG
BsrFI 659 GCCGGC [GA]^CCGG[CT]
1135 GCCGGC
BstYI 349 AGATCC [GA]^GATC[CT]
552 AGATCC
Cfr10I 659 GCCGGC [GA]^CCGG[CT]
1135 GCCGGC
DsaI 182 CCACGG C^C[GA][CT]GG
1600 CCACGG
EaeI 312 TGGCCG [CT]^GGCC[GA]
657 CGGCCG
EcoT14I 28 CCTTGG C^C[AT][AT]GG
493 CCAAGG
FauI 1094 CCCGC CCCGC(4/6)
419 GCGGG
HaeII 6 GGCGCT [GA]GCGC^[CT]
137 GGCGCT
MaeI 247 CTAG C^TAG
329 CTAG
MaeII 263 ACGT A^CGT
1074 ACGT
MaeIII 1376 GTGAC ^GT[ACGT]AC
1562 GTGAC
MflI 349 AGATCC [GA]^GATC[CT]
552 AGATCC
NaeI 659 GCCGGC GCC^GGC
1135 GCCGGC
NgoMIV 659 GCCGGC G^CCGGC
1135 GCCGGC
PflMI 1542 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1559 CCAGTGACTGG
PstI 206 CTGCAG CTGCA^G
1512 CTGCAG
StyI 28 CCTTGG C^C[AT][AT]GG
493 CCAAGG
Tsp45I 1376 GTGAC ^GT[GC]AC
1562 GTGAC
Van91I 1542 CCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1559 CCAGTGACTGG
XcmI 1538 CCAACCACAACATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1555 CCACCCAGTGACTGG
XhoII 349 AGATCC [GA]^GATC[CT]
552 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 61 enzymes) #
#########################################
AciI AfaI AluI AvaII BanII
BbvI BcnI BmyI BsaJI BseRI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII EcoRII Fnu4HI
FokI HaeIII HapII HhaI HinP1I
HinfI HpaII HphI ItaI MboI
MboII MnlI MseI MspA1I MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV PleI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI Tru9I TseI Tsp509I TspRI
TthHB8I