Restriction map for
pFN21AE1386
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AcsI AcyI AflII AflIII
AgeI Alw44I AlwI AlwNI AocI
Aor51HI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspHI AspI
AvaI AviII AvrII BalI BamHI
BbeI BbrPI BclI BcnI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiHKAI BsiWI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstYI BstZ17I Bsu36I
CelII CfoI Cfr10I ClaI DpnII
DraI DraIII DrdI EaeI EagI
EclXI Eco47III Eco52I Eco81I EcoNI
EcoO65I EcoRI EcoRV FauI FbaI
FseI FspI HaeII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HindIII HinfI HpaI HpaII KasI
KpnI KspI MaeIII MamI MboI
MfeI MflI MluI MluNI MroI
MscI MseI MspI MunI MvnI
NaeI NarI NciI NcoI NdeI
NdeII NgoMIV NheI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI SacI SacII SalI Sau3AI
SbfI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI StuI SwaI
TfiI Tru9I Tsp45I Tth111I XbaI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 36 enzymes) #
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AccI 325 GTCTAC GT^[AC][GT]AC
AhdI 138 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
ApaI 92 GGGCCC GGGCC^C
AspEI 138 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 40 GGCACC G^G[CT][GA]CC
BcgI 314 GCATCTACTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 465 GTATCC GTATCC(6/5)
BsgI 490 CTGCAC GTGCAG(16/14)
BsmBI 16 CGTCTC CGTCTC(1/5)
BsmFI 301 GTCCC GGGAC(10/14)
BspMI 186 ACCTGC ACCTGC(4/8)
BstXI 549 CCACACAGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 215 GCAAGC GC[ACGT]^[ACGT]GC
CpoI 248 CGGACCG CG^G[AT]CCG
DraII 91 GGGGCCC [GA]G^G[ACGT]CC[CT]
DsaI 504 CCACGG C^C[GA][CT]GG
Eam1105I 138 GACTGCCCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoO109I 91 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 160 ATGCAT ATGCA^T
MaeII 15 ACGT A^CGT
MslI 550 CACACAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 128 CAGCTG C[AC]G^C[GT]G
NsiI 160 ATGCAT ATGCA^T
PflMI 453 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PstI 475 CTGCAG CTGCA^G
PvuII 128 CAGCTG CAG^CTG
RsrII 248 CGGACCG CG^G[AT]CCG
SapI 408 GCTCTTC GCTCTTC(1/4)
ScaI 586 AGTACT AGT^ACT
SfcI 475 CTGCAG C^T[GA][CT]AG
SmlI 279 CTTGAG C^T[CT][GA]AG
TaqI 496 TCGA T^CGA
Tsp509I 289 AATT ^AATT
TthHB8I 496 TCGA T^CGA
Van91I 453 CCAGAAGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 96 CCAGGGGCTCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 22 enzymes) #
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BanII 92 GGGCCC G[GA]GC[CT]^C
100 GGGCTC
BbsI 340 GTCTTC GAAGAC(2/6)
592 GTCTTC
BbvCI 444 CCTCAGC CCTCAGC(-5/-2)
576 CCTCAGC
BfaI 571 CTAG C^TAG
584 CTAG
BmyI 92 GGGCCC G[AGT]GC[ACT]^C
100 GGGCTC
BpmI 262 CTCCAG CTGGAG(16/14)
234 CTGGAG
BpuAI 340 GTCTTC GAAGAC(2/6)
592 GTCTTC
BsaI 206 GAGACC GGTCTC(1/5)
533 GAGACC
Bsp1286I 92 GGGCCC G[AGT]GC[ACT]^C
100 GGGCTC
BsrI 308 CCAGT ACTGG(1/-1)
420 CCAGT
BstF5I 561 CATCC GGATG(2/0)
267 GGATG
DdeI 445 CTCAG C^T[ACGT]AG
577 CTCAG
EarI 19 CTCTTC CTCTTC(1/4)
409 CTCTTC
EcoT14I 28 CCAAGG C^C[AT][AT]GG
365 CCAAGG
FokI 561 CATCC GGATG(9/13)
267 GGATG
HphI 195 TCACC GGTGA(8/7)
524 TCACC
Ksp632I 19 CTCTTC CTCTTC(1/4)
409 CTCTTC
MaeI 571 CTAG C^TAG
584 CTAG
MwoI 75 GCTGGTTCTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
133 GCCCAGACTGC
NlaIII 397 CATG CATG^
433 CATG
StyI 28 CCAAGG C^C[AT][AT]GG
365 CCAAGG
TspRI 227 CACTG CA[GC]TG[ACGT][ACGT]^
309 CAGTG
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# 3 OR MORE CUT ENZYMES ( 25 enzymes) #
#########################################
AfaI AluI AvaII BbvI BsaJI
BseRI BsiYI BslI BsmAI BssKI
BstNI Cfr13I EcoRII Fnu4HI HaeIII
ItaI MboII MnlI MvaI NlaIV
RsaI Sau96I ScrFI SfaNI TseI