##################################
# NO CUT ENZYMES (  210 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcyI        AfaI        AflII       AflIII      
AgeI        AhdI        AluI        Alw44I      AlwI        
AlwNI       AocI        Aor51HI     ApaI        ApaLI       
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaII       AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbsI        BbvCI       BbvI        
BcgI        BciVI       BclI        BcnI        BfrI        
BglII       BlnI        BlpI        BmyI        Bpu1102I    
BpuAI       BsaAI       BsaBI       BsaHI       BsaJI       
BsaWI       BseAI       BsgI        BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        Bsp1286I    Bsp1407I    
BspDI       BspEI       BspHI       BspLU11I    BsrBI       
BsrDI       BsrFI       BsrGI       BssHII      BssKI       
BssSI       Bst1107I    BstAPI      BstBI       BstEII      
BstF5I      BstNI       BstPI       BstUI       BstYI       
BstZ17I     Bsu36I      CelII       CfoI        Cfr10I      
ClaI        CpoI        DpnII       DraI        DraII       
DraIII      DrdI        DsaI        EaeI        EagI        
Eam1105I    EclXI       Eco47III    Eco52I      Eco81I      
EcoNI       EcoO109I    EcoO65I     EcoRII      EcoRV       
EcoT14I     EcoT22I     FbaI        Fnu4HI      FokI        
FseI        FspI        HaeII       HhaI        Hin1I       
HinP1I      HincII      HindII      HindIII     HpaI        
ItaI        KasI        KpnI        KspI        MaeIII      
MamI        MboI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MspA1I      
MunI        MvaI        MvnI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIII      NlaIV       NotI        NruI        
NsiI        NspI        NspV        PacI        PaeR7I      
PflFI       PflMI       PinAI       PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsaI        
RsrII       SacI        SacII       SalI        SapI        
Sau3AI      SbfI        ScaI        ScrFI       SexAI       
SfoI        SfuI        SgrAI       SmaI        SmlI        
SnaBI       SphI        Sse8387I    SspBI       SspI        
StuI        StyI        SwaI        Tru9I       TseI        
Tsp45I      Tth111I     Van91I      XbaI        XcmI        
XhoI        XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   33 enzymes) #
###################################

AcsI          274  GAATTC               [GA]^AATT[CT]
ApoI          274  GAATTC               [GA]^AATT[CT]
AvaI          233  CCCGAG               C^[CT]CG[GA]G
BfaI          169  CTAG                 C^TAG
BpmI          206  CTCCAG               CTGGAG(16/14)
BsaI           97  GAGACC               GGTCTC(1/5)
BsmAI          97  GAGAC                GTCTC(1/5)
BsoBI         233  CCCGAG               C^[CT]CG[GA]G
BspMI         126  ACCTGC               ACCTGC(4/8)
BstXI         164  CCATACTAGTGG         CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I          23  GCAGGC               GC[ACGT]^[ACGT]GC
Cfr13I          3  GGGCC                G^G[ACGT]CC
EarI           30  GAAGAG               CTCTTC(1/4)
EcoRI         274  GAATTC               G^AATTC
FauI          120  CCCGC                CCCGC(4/6)
HapII          94  CCGG                 C^CGG
HgaI           38  GCGTC                GACGC(5/10)
HpaII          94  CCGG                 C^CGG
HphI          219  GGTGA                GGTGA(8/7)
Ksp632I        30  GAAGAG               CTCTTC(1/4)
MaeI          169  CTAG                 C^TAG
MaeII         191  ACGT                 A^CGT
MboII          30  GAAGA                GAAGA(8/7)
MslI          165  CATACTAGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI           94  CCGG                 C^CGG
Sau96I          3  GGGCC                G^G[ACGT]CC
SfaNI          90  GATGC                GCATC(5/9)
SfcI          180  CTACAG               C^T[GA][CT]AG
SpeI          168  ACTAGT               A^CTAGT
TaqI          160  TCGA                 T^CGA
Tsp509I       275  AATT                 ^AATT
TspRI          15  CAGTG                CA[GC]TG[ACGT][ACGT]^
TthHB8I       160  TCGA                 T^CGA


###################################
# TWO CUT ENZYMES (    8 enzymes) #
###################################

AciI           21  CCGC                 CCGC(-3/-1)
              121  CCGC                
BglI           61  GCCAACGAGGC          GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
               70  GCCAATGAGGC         
BsmFI         110  GGGAC                GGGAC(10/14)
               40  GTCCC               
DdeI          114  CTCAG                C^T[ACGT]AG
              152  CTGAG               
MwoI           61  GCCAACGAGGC          GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
               70  GCCAATGAGGC         
PleI          112  GACTC                GAGTC(4/5)
              154  GAGTC               
SfiI           60  GGCCAACGAGGCC        GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
               69  GGCCAATGAGGCC       
TfiI           49  GAATC                G^A[AT]TC
              245  GAATC               


#########################################
# 3 OR MORE CUT ENZYMES (    7 enzymes) #
#########################################

BseRI       BsiYI       BslI        BsrI        HaeIII      
HinfI       MnlI