##################################
# NO CUT ENZYMES (  122 enzymes) #
##################################

AatII       Acc65I      AccI        AccIII      AclI        
AcsI        AflII       AgeI        AhdI        ApaI        
ApoI        AscI        AseI        AsnI        Asp700I     
Asp718I     AspEI       AviII       BalI        BamHI       
BbrPI       BbsI        BcgI        BciVI       BclI        
BfrI        BglI        BglII       BlpI        Bpu1102I    
BpuAI       BsaBI       BsaWI       BseAI       BsiEI       
BsiWI       BsmBI       Bsp1407I    BspDI       BspEI       
BspHI       BsrGI       BssHII      Bst1107I    BstBI       
BstEII      BstPI       BstYI       BstZ17I     CelII       
ClaI        CpoI        DraI        DrdI        EagI        
Eam1105I    EclXI       Eco52I      EcoO65I     EcoRI       
EcoRV       EcoT22I     FbaI        FseI        FspI        
HincII      HindII      HindIII     HpaI        KpnI        
KspI        MamI        MfeI        MflI        MluI        
MluNI       MroI        MscI        MseI        MunI        
NdeI        NheI        NotI        NruI        NsiI        
NspV        PacI        PaeR7I      PflMI       PinAI       
PmaCI       PmeI        PmlI        PshAI       PshBI       
Psp1406I    PvuI        RcaI        RsrII       SacII       
SalI        SbfI        ScaI        SexAI       SfiI        
SfuI        SgrAI       SpeI        Sse8387I    SspBI       
SspI        StuI        SwaI        TfiI        Tru9I       
Tsp509I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmnI        


###################################
# ONE CUT ENZYMES (   32 enzymes) #
###################################

AflIII        770  ACATGT               A^C[GA][CT]GT
Alw44I        313  GTGCAC               G^TGCAC
AlwI         1263  GATCC                GGATC(4/5)
AocI           99  CCTGAGG              CC^T[ACGT]AGG
Aor51HI      1274  AGCGCT               AGC^GCT
ApaLI         313  GTGCAC               G^TGCAC
AspI         1054  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
AvrII          75  CCTAGG               C^CTAGG
BbvCI          93  CCTCAGC              CCTCAGC(-5/-2)
BlnI           75  CCTAGG               C^CTAGG
BsaI         1705  GAGACC               GGTCTC(1/5)
BsmI         1140  GAATGC               GAATGC(1/-1)
BspLU11I      770  ACATGT               A^CATGT
BsrBI        1750  GAGCGG               CCGCTC(-3/-3)
BsrDI        1590  CATTGC               GCAATG(2/0)
Bsu36I         99  CCTGAGG              CC^T[ACGT]AGG
DraIII        127  CACGGCGTG            CAC[ACGT][ACGT][ACGT]^GTG
Eco47III     1274  AGCGCT               AGC^GCT
Eco81I         99  CCTGAGG              CC^T[ACGT]AGG
MaeIII        247  GTGAC                ^GT[ACGT]AC
MslI         1062  CATGCAGGTG           CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI         1322  GCCGGC               GCC^GGC
NgoMIV       1322  GCCGGC               G^CCGGC
PflFI        1054  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
SmaI          863  CCCGGG               CCC^GGG
SmlI          252  CTTGAG               C^T[CT][GA]AG
SnaBI         508  TACGTA               TAC^GTA
SphI          548  GCATGC               GCATG^C
Tsp45I        247  GTGAC                ^GT[GC]AC
Tth111I      1054  GACAAGGTC            GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI         863  CCCGGG               C^CCGGG
XmaI          863  CCCGGG               C^CCGGG


###################################
# TWO CUT ENZYMES (   15 enzymes) #
###################################

BbeI         1328  GGCGCC               GGCGC^C
             1402  GGCGCC              
BfaI           46  CTAG                 C^TAG
               76  CTAG                
BsaAI         184  TACGTG               [CT]AC^GT[GA]
              508  TACGTA              
BseRI        1396  GAGGAG               GAGGAG(10/8)
             1567  GAGGAG              
BsgI         1189  CTGCAC               GTGCAG(16/14)
              470  GTGCAG              
BspMI         597  GCAGGT               ACCTGC(4/8)
             1065  GCAGGT              
BsrFI         809  GCCGGT               [GA]^CCGG[CT]
             1322  GCCGGC              
BssSI         847  CACGAG               CACGAG(-5/-1)
              979  CACGAG              
Cfr10I        809  GCCGGT               [GA]^CCGG[CT]
             1322  GCCGGC              
KasI         1328  GGCGCC               G^GCGCC
             1402  GGCGCC              
MaeI           46  CTAG                 C^TAG
               76  CTAG                
NarI         1328  GGCGCC               GG^CGCC
             1402  GGCGCC              
SfoI         1328  GGCGCC               GGC^GCC
             1402  GGCGCC              
TaqI          620  TCGA                 T^CGA
             1052  TCGA                
TthHB8I       620  TCGA                 T^CGA
             1052  TCGA                


#########################################
# 3 OR MORE CUT ENZYMES (   89 enzymes) #
#########################################

AccII       AciI        AcyI        AfaI        AluI        
AlwNI       AspHI       AvaI        AvaII       BanI        
BanII       BbvI        BcnI        BmyI        BpmI        
BsaHI       BsaJI       BsiHKAI     BsiYI       BslI        
BsmAI       BsmFI       BsoBI       Bsp1286I    BsrI        
BssKI       BstAPI      BstF5I      BstNI       BstUI       
BstXI       Cac8I       CfoI        Cfr13I      DdeI        
DpnII       DraII       DsaI        EaeI        EarI        
EcoNI       EcoO109I    EcoRII      EcoT14I     FauI        
Fnu4HI      FokI        HaeII       HaeIII      HapII       
HgaI        HhaI        Hin1I       HinP1I      HinfI       
HpaII       HphI        ItaI        Ksp632I     MaeII       
MboI        MboII       MnlI        MspA1I      MspI        
MvaI        MvnI        MwoI        NciI        NcoI        
NdeII       NlaIII      NlaIV       NspI        PleI        
PpuMI       PstI        PvuII       RsaI        SacI        
SapI        Sau3AI      Sau96I      ScrFI       SfaNI       
SfcI        StyI        TseI        TspRI