Restriction map for
pFN21AE5404
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# NO CUT ENZYMES ( 162 enzymes) #
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Acc65I AccI AccII AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspHI AspI AvaI AvaII AvrII
BalI BamHI BanII BbeI BbrPI
BbsI BbvCI BcgI BciVI BfrI
BglII BlnI BlpI BmyI Bpu1102I
BpuAI BsaAI BsaBI BseAI BsgI
BsiEI BsiHKAI BsiWI BsmBI BsoBI
Bsp1286I Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstXI BstYI BstZ17I
Bsu36I CelII Cfr10I ClaI CpoI
DraI DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV EcoT22I
FseI HaeII HindIII HpaI KasI
KpnI Ksp632I KspI MamI MflI
MluI MluNI MroI MscI MvnI
NaeI NarI NcoI NgoMIV NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfaNI SfiI
SfoI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SspI StuI SwaI Tth111I Van91I
XbaI XcmI XhoI XhoII XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AatII 7 GACGTC GACGT^C
AcsI 377 GAATTT [GA]^AATT[CT]
AcyI 7 GACGTC G[GA]^CG[CT]C
AlwI 840 GATCC GGATC(4/5)
AlwNI 670 CAGGTGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 377 GAATTT [GA]^AATT[CT]
AviII 616 TGCGCA TGC^GCA
BanI 865 GGTGCC G^G[CT][GA]CC
BclI 155 TGATCA T^GATCA
BcnI 843 CCCGG CC^[GC]GG
BglI 55 GCCTGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 7 GACGTC G[GA]^CG[CT]C
BsaI 532 GGTCTC GGTCTC(1/5)
BsaWI 438 ACCGGA [AT]^CCGG[AT]
BseRI 291 GAGGAG GAGGAG(10/8)
BsmI 398 GAATGC GAATGC(1/-1)
BsrBI 277 GAGCGG CCGCTC(-3/-3)
CfoI 617 GCGC GCG^C
DraII 629 AGGCCCT [GA]G^G[ACGT]CC[CT]
DraIII 892 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoO109I 629 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 823 GCGGG CCCGC(4/6)
FbaI 155 TGATCA T^GATCA
FspI 616 TGCGCA TGC^GCA
HgaI 240 GACGC GACGC(5/10)
HhaI 617 GCGC GCG^C
Hin1I 7 GACGTC G[GA]^CG[CT]C
HinP1I 617 GCGC G^CGC
HincII 385 GTCAAC GT[CT]^[GA]AC
HindII 385 GTCAAC GT[CT]^[GA]AC
MfeI 779 CAATTG C^AATTG
MseI 347 TTAA T^TAA
MslI 666 CATCCAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 861 CAGCGG C[AC]G^C[GT]G
MunI 779 CAATTG C^AATTG
NciI 843 CCCGG CC^[GC]GG
NheI 201 GCTAGC G^CTAGC
NlaIV 865 GGTGCC GG[ACGT]^[ACGT]CC
Tru9I 347 TTAA T^TAA
Tsp45I 775 GTCAC ^GT[GC]AC
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# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AfaI 189 GTAC GT^AC
591 GTAC
BfaI 202 CTAG C^TAG
253 CTAG
BpmI 81 CTCCAG CTGGAG(16/14)
161 CTGGAG
BsmAI 533 GTCTC GTCTC(1/5)
691 GTCTC
BsmFI 5 GGGAC GGGAC(10/14)
141 GGGAC
BsrDI 261 CATTGC GCAATG(2/0)
700 GCAATG
BsrI 104 ACTGG ACTGG(1/-1)
160 ACTGG
Cfr13I 630 GGCCC G^G[ACGT]CC
851 GGGCC
EcoNI 633 CCTGCAAGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
869 CCTTCCAAAGG
HphI 787 GGTGA GGTGA(8/7)
473 TCACC
MaeI 202 CTAG C^TAG
253 CTAG
MaeII 8 ACGT A^CGT
210 ACGT
MaeIII 467 GTAAC ^GT[ACGT]AC
775 GTCAC
NdeI 511 CATATG CA^TATG
683 CATATG
NlaIII 588 CATG CATG^
811 CATG
PleI 215 GACTC GAGTC(4/5)
760 GAGTC
RsaI 189 GTAC GT^AC
591 GTAC
Sau96I 630 GGCCC G^G[ACGT]CC
851 GGGCC
SfcI 284 CTACAG C^T[GA][CT]AG
858 CTACAG
TfiI 77 GATTC G^A[AT]TC
517 GAATC
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# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
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AciI AluI BbvI BsaJI BsiYI
BslI BssKI BstF5I BstNI Cac8I
DdeI DpnII EcoRII EcoT14I Fnu4HI
FokI HaeIII HapII HinfI HpaII
ItaI MboI MboII MnlI MspI
MvaI MwoI NdeII Sau3AI ScrFI
StyI TaqI TseI Tsp509I TspRI
TthHB8I