Restriction map for
pFN21AE1318
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# NO CUT ENZYMES ( 97 enzymes) #
##################################
AatII Acc65I AccIII AflII AgeI
AocI ApaI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BbeI BbrPI BciVI BclI BfrI
BlnI BsaAI BseAI BsiEI BsiWI
Bsp1407I BspDI BspEI BspLU11I BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstZ17I Bsu36I ClaI CpoI DraI
EagI EclXI Eco52I Eco81I EcoO65I
EcoRI EcoRV FbaI FseI FspI
HpaI KasI KpnI KspI MluI
MroI NarI NdeI NotI NruI
NspI NspV PacI PaeR7I PflFI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI PvuI RsrII SacII
SapI SbfI ScaI SexAI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI Tth111I XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 1872 CGCG CG^CG
AclI 1334 AACGTT AA^CGTT
AcsI 3337 GAATTT [GA]^AATT[CT]
AflIII 1796 ACGTGT A^C[GA][CT]GT
AhdI 2144 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 3272 GTGCAC G^TGCAC
ApaLI 3272 GTGCAC G^TGCAC
ApoI 3337 GAATTT [GA]^AATT[CT]
AspEI 2144 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BanI 589 GGTGCC G^G[CT][GA]CC
BglI 1854 GCCCAGCGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlpI 3452 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 3452 GCTTAGC GC^T[ACGT]AGC
BsaWI 3089 ACCGGA [AT]^CCGG[AT]
BspMI 1844 ACCTGC ACCTGC(4/8)
BsrBI 406 GAGCGG CCGCTC(-3/-3)
BstUI 1872 CGCG CG^CG
CelII 3452 GCTTAGC GC^T[ACGT]AGC
DraII 39 AGGCCCC [GA]G^G[ACGT]CC[CT]
DraIII 995 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 2832 GACCAGCCCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 2144 GACTGGGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 46 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 39 AGGCCCC [GA]G^G[ACGT]CC[CT]
EcoT22I 1066 ATGCAT ATGCA^T
HindIII 3396 AAGCTT A^AGCTT
MfeI 3185 CAATTG C^AATTG
MunI 3185 CAATTG C^AATTG
MvnI 1872 CGCG CG^CG
NaeI 1673 GCCGGC GCC^GGC
NgoMIV 1673 GCCGGC G^CCGGC
NheI 2068 GCTAGC G^CTAGC
NsiI 1066 ATGCAT ATGCA^T
PflMI 1188 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1334 AACGTT AA^CGTT
PstI 1288 CTGCAG CTGCA^G
SalI 3025 GTCGAC G^TCGAC
SmlI 170 CTCAAG C^T[CT][GA]AG
Van91I 1188 CCAGAGGGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 2757 CCAGCGTGAGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccI 2965 GTAGAC GT^[AC][GT]AC
3025 GTCGAC
AlwNI 1138 CAGACACTG CAG[ACGT][ACGT][ACGT]^CTG
1343 CAGACACTG
Aor51HI 1106 AGCGCT AGC^GCT
1200 AGCGCT
AvaI 2129 CTCGGG C^[CT]CG[GA]G
2235 CCCGAG
BalI 1620 TGGCCA TGG^CCA
2807 TGGCCA
BamHI 2111 GGATCC G^GATCC
3176 GGATCC
BbsI 739 GAAGAC GAAGAC(2/6)
3510 GTCTTC
BbvCI 1830 CCTCAGC CCTCAGC(-5/-2)
2799 GCTGAGG
BcgI 1782 CGAGAGTGATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
3264 CGACTTCAGTGC
BglII 709 AGATCT A^GATCT
876 AGATCT
BpuAI 739 GAAGAC GAAGAC(2/6)
3510 GTCTTC
BsaBI 1486 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2766 GATTGGCATC
BsaI 120 GGTCTC GGTCTC(1/5)
410 GGTCTC
BsmBI 1156 GAGACG CGTCTC(1/5)
2500 GAGACG
BsmI 385 GAATGC GAATGC(1/-1)
1777 GCATTC
BsoBI 2129 CTCGGG C^[CT]CG[GA]G
2235 CCCGAG
BspHI 216 TCATGA T^CATGA
2153 TCATGA
BsrDI 3155 GCAATG GCAATG(2/0)
3551 GCAATG
BsrFI 1673 GCCGGC [GA]^CCGG[CT]
2255 ACCGGC
BstAPI 1125 GCAAAAGCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2583 GCATGAATTGC
BstXI 464 CCAGCGAAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
2972 CCAGGATGCTGG
Cfr10I 1673 GCCGGC [GA]^CCGG[CT]
2255 ACCGGC
Eco47III 1106 AGCGCT AGC^GCT
1200 AGCGCT
MamI 1486 GATAAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
2766 GATTGGCATC
MluNI 1620 TGGCCA TGG^CCA
2807 TGGCCA
MscI 1620 TGGCCA TGG^CCA
2807 TGGCCA
MslI 1008 CACATCTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
3410 CACCTTCATG
PvuII 1903 CAGCTG CAG^CTG
3013 CAGCTG
RcaI 216 TCATGA T^CATGA
2153 TCATGA
Tsp45I 838 GTGAC ^GT[GC]AC
3496 GTGAC
XbaI 2004 TCTAGA T^CTAGA
2857 TCTAGA
#########################################
# 3 OR MORE CUT ENZYMES ( 90 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BfaI BmyI BpmI BsaHI BsaJI
BseRI BsgI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BssSI BstF5I BstNI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DsaI
EaeI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeII HaeIII HapII
HgaI HhaI Hin1I HinP1I HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeI MaeII MaeIII MboI
MboII MflI MnlI MseI MspA1I
MspI MvaI MwoI NciI NcoI
NdeII NlaIII NlaIV PleI RsaI
SacI Sau3AI Sau96I ScrFI SfaNI
SfcI StyI TaqI TfiI Tru9I
TseI Tsp509I TspRI TthHB8I XhoII