Restriction map for
pFN21AE0974
##################################
# NO CUT ENZYMES ( 104 enzymes) #
##################################
AatII Acc65I AccIII AclI AcsI
AcyI AflII AgeI AhdI Aor51HI
ApoI AscI AseI AsnI Asp718I
AspEI AvrII BamHI BbeI BbvCI
BcgI BciVI BfrI BglII BlnI
BlpI Bpu1102I BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BspLU11I BsrBI
BsrDI BsrGI BssHII BstAPI BstBI
CelII ClaI CpoI DraI DraIII
DrdI EagI Eam1105I EclXI Eco47III
Eco52I EcoRI FseI Hin1I HindIII
KasI KpnI KspI MfeI MluI
MroI MunI NarI NcoI NdeI
NheI NotI NruI NspI NspV
PacI PaeR7I PflMI PinAI PleI
PmeI PshBI Psp1406I PvuI RcaI
RsrII SacII SbfI SexAI SfiI
SfoI SfuI SgrAI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
Tsp45I Van91I XbaI XhoI
###################################
# ONE CUT ENZYMES ( 56 enzymes) #
###################################
AccII 981 CGCG CG^CG
Alw44I 1075 GTGCAC G^TGCAC
ApaI 1559 GGGCCC GGGCC^C
ApaLI 1075 GTGCAC G^TGCAC
Asp700I 247 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 872 TGCGCA TGC^GCA
BbrPI 1405 CACGTG CAC^GTG
BbsI 298 GAAGAC GAAGAC(2/6)
BclI 743 TGATCA T^GATCA
BglI 991 GCCGGCCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 298 GAAGAC GAAGAC(2/6)
BsaAI 1405 CACGTG [CT]AC^GT[GA]
BsaBI 646 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaI 1498 GAGACC GGTCTC(1/5)
BseRI 52 GAGGAG GAGGAG(10/8)
BsgI 18 CTGCAC GTGCAG(16/14)
Bst1107I 347 GTATAC GTA^TAC
BstEII 1303 GGTAACC G^GT[ACGT]ACC
BstPI 1303 GGTAACC G^GT[ACGT]ACC
BstUI 981 CGCG CG^CG
BstXI 1404 CCACGTGATTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 564 AGATCC [GA]^GATC[CT]
BstZ17I 347 GTATAC GTA^TAC
EcoNI 1308 CCTCGTGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1303 GGTAACC G^GT[ACGT]ACC
EcoRV 586 GATATC GAT^ATC
EcoT14I 1299 CCTTGG C^C[AT][AT]GG
EcoT22I 844 ATGCAT ATGCA^T
FbaI 743 TGATCA T^GATCA
FspI 872 TGCGCA TGC^GCA
HaeII 985 AGCGCC [GA]GCGC^[CT]
HgaI 1420 GACGC GACGC(5/10)
HpaI 370 GTTAAC GTT^AAC
MamI 646 GATGAGGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 564 AGATCC [GA]^GATC[CT]
MvnI 981 CGCG CG^CG
NaeI 991 GCCGGC GCC^GGC
NgoMIV 991 GCCGGC G^CCGGC
NsiI 844 ATGCAT ATGCA^T
PflFI 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 1405 CACGTG CAC^GTG
PmlI 1405 CACGTG CAC^GTG
PshAI 1500 GACCCGGGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 1576 CTGCAG CTGCA^G
PvuII 1213 CAGCTG CAG^CTG
SacI 957 GAGCTC GAGCT^C
SalI 194 GTCGAC G^TCGAC
SapI 45 GCTCTTC GCTCTTC(1/4)
ScaI 317 AGTACT AGT^ACT
StyI 1299 CCTTGG C^C[AT][AT]GG
Tsp509I 146 AATT ^AATT
Tth111I 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 700 CCATCACCAGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 564 AGATCC [GA]^GATC[CT]
XmnI 247 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 32 enzymes) #
###################################
AccI 194 GTCGAC GT^[AC][GT]AC
347 GTATAC
AflIII 22 ACACGT A^C[GA][CT]GT
1398 ACACGT
AocI 547 CCTGAGG CC^T[ACGT]AGG
1291 CCTGAGG
AspHI 957 GAGCTC G[AT]GC[AT]^C
1075 GTGCAC
AvaI 155 CCCGGG C^[CT]CG[GA]G
1502 CCCGGG
BalI 2 TGGCCA TGG^CCA
308 TGGCCA
BfaI 172 CTAG C^TAG
478 CTAG
BsiHKAI 957 GAGCTC G[AT]GC[AT]^C
1075 GTGCAC
BsoBI 155 CCCGGG C^[CT]CG[GA]G
1502 CCCGGG
BsrFI 280 ACCGGC [GA]^CCGG[CT]
991 GCCGGC
BsrI 1452 CCAGT ACTGG(1/-1)
1488 CCAGT
BssSI 262 CTCGTG CACGAG(-5/-1)
1309 CTCGTG
Bsu36I 547 CCTGAGG CC^T[ACGT]AGG
1291 CCTGAGG
Cfr10I 280 ACCGGC [GA]^CCGG[CT]
991 GCCGGC
DsaI 808 CCGTGG C^C[GA][CT]GG
827 CCACGG
EarI 46 CTCTTC CTCTTC(1/4)
1495 GAAGAG
Eco81I 547 CCTGAGG CC^T[ACGT]AGG
1291 CCTGAGG
HinfI 357 GATTC G^A[ACGT]TC
1347 GAATC
Ksp632I 46 CTCTTC CTCTTC(1/4)
1495 GAAGAG
MaeI 172 CTAG C^TAG
478 CTAG
MluNI 2 TGGCCA TGG^CCA
308 TGGCCA
MscI 2 TGGCCA TGG^CCA
308 TGGCCA
MseI 371 TTAA T^TAA
1199 TTAA
MspA1I 1213 CAGCTG C[AC]G^C[GT]G
1573 CCGCTG
PpuMI 404 GGGACCT [GA]G^G[AT]CC[CT]
1169 AGGACCT
SmaI 155 CCCGGG CCC^GGG
1502 CCCGGG
SmlI 555 CTTGAG C^T[CT][GA]AG
799 CTCAAG
StuI 1247 AGGCCT AGG^CCT
1272 AGGCCT
TfiI 357 GATTC G^A[AT]TC
1347 GAATC
Tru9I 371 TTAA T^TAA
1199 TTAA
XmaCI 155 CCCGGG C^CCGGG
1502 CCCGGG
XmaI 155 CCCGGG C^CCGGG
1502 CCCGGG
#########################################
# 3 OR MORE CUT ENZYMES ( 66 enzymes) #
#########################################
AciI AfaI AluI AlwI AlwNI
AvaII BanI BanII BbvI BcnI
BmyI BpmI BsaJI BsiYI BslI
BsmAI BsmFI Bsp1286I BspMI BssKI
BstF5I BstNI Cac8I CfoI Cfr13I
DdeI DpnII DraII EaeI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HincII HindII
HpaII HphI ItaI MaeII MaeIII
MboI MboII MnlI MslI MspI
MvaI MwoI NciI NdeII NlaIII
NlaIV RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI TaqI TseI TspRI
TthHB8I