Restriction map for
pFN21AE1537
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# NO CUT ENZYMES ( 81 enzymes) #
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Acc65I AccI AclI AflII AflIII
AgeI Alw44I AocI Aor51HI ApaLI
AscI AseI AsnI Asp718I AvrII
BcgI BciVI BclI BfrI BglII
BlnI BsaBI BsiWI BspDI BspHI
BspLU11I BssHII Bst1107I BstBI BstEII
BstPI BstZ17I Bsu36I ClaI DraI
DraIII DrdI EagI EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KpnI MamI MfeI MluI MseI
MunI NdeI NheI NotI NruI
NsiI NspV PacI PinAI PmeI
PshAI PshBI Psp1406I RcaI SalI
SbfI ScaI SfuI SgrAI SnaBI
SpeI Sse8387I SspI SwaI Tru9I
XbaI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AatII 2388 GACGTC GACGT^C
AccIII 734 TCCGGA T^CCGGA
AhdI 709 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 709 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspI 2229 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 1295 TGCGCA TGC^GCA
BalI 2153 TGGCCA TGG^CCA
BamHI 2278 GGATCC G^GATCC
BfaI 374 CTAG C^TAG
BseAI 734 TCCGGA T^CCGGA
Bsp1407I 671 TGTACA T^GTACA
BspEI 734 TCCGGA T^CCGGA
BsrDI 2372 GCAATG GCAATG(2/0)
BsrGI 671 TGTACA T^GTACA
BssSI 1948 CACGAG CACGAG(-5/-1)
BstXI 1957 CCACTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 2278 GGATCC [GA]^GATC[CT]
CpoI 2407 CGGACCG CG^G[AT]CCG
Eam1105I 709 GACAAGAAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FspI 1295 TGCGCA TGC^GCA
KspI 1639 CCGCGG CCGC^GG
MaeI 374 CTAG C^TAG
MflI 2278 GGATCC [GA]^GATC[CT]
MluNI 2153 TGGCCA TGG^CCA
MroI 734 TCCGGA T^CCGGA
MscI 2153 TGGCCA TGG^CCA
NaeI 452 GCCGGC GCC^GGC
NcoI 1334 CCATGG C^CATGG
NgoMIV 452 GCCGGC G^CCGGC
PaeR7I 2549 CTCGAG C^TCGAG
PflFI 2229 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PvuI 1425 CGATCG CGAT^CG
RsrII 2407 CGGACCG CG^G[AT]CCG
SacI 1396 GAGCTC GAGCT^C
SacII 1639 CCGCGG CCGC^GG
SexAI 1011 ACCAGGT A^CC[AT]GGT
SmaI 563 CCCGGG CCC^GGG
SspBI 671 TGTACA T^GTACA
StuI 281 AGGCCT AGG^CCT
TfiI 2417 GAATC G^A[AT]TC
Tth111I 2229 GACTGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoI 2549 CTCGAG C^TCGAG
XhoII 2278 GGATCC [GA]^GATC[CT]
XmaCI 563 CCCGGG C^CCGGG
XmaI 563 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
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AccII 1640 CGCG CG^CG
2146 CGCG
AcsI 1790 AAATTC [GA]^AATT[CT]
2668 GAATTT
ApoI 1790 AAATTC [GA]^AATT[CT]
2668 GAATTT
AspHI 1396 GAGCTC G[AT]GC[AT]^C
2255 GTGCTC
BsaI 2635 GAGACC GGTCTC(1/5)
2682 GAGACC
BsiHKAI 1396 GAGCTC G[AT]GC[AT]^C
2255 GTGCTC
BsmI 1342 GAATGC GAATGC(1/-1)
1410 GCATTC
BstAPI 1221 GCACAGCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1998 GCATGTGCTGC
BstUI 1640 CGCG CG^CG
2146 CGCG
EaeI 2153 TGGCCA [CT]^GGCC[GA]
2379 TGGCCG
EcoNI 144 CCTGGAGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1461 CCTCATCGAGG
HincII 655 GTCAAC GT[CT]^[GA]AC
1804 GTCAAC
HindII 655 GTCAAC GT[CT]^[GA]AC
1804 GTCAAC
MvnI 1640 CGCG CG^CG
2146 CGCG
SfiI 273 GGCCAAGAAGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
2452 GGCCCCGAGGGCC
SphI 237 GCATGC GCATG^C
1298 GCATGC
#########################################
# 3 OR MORE CUT ENZYMES ( 116 enzymes) #
#########################################
AciI AcyI AfaI AluI AlwI
AlwNI ApaI Asp700I AvaI AvaII
BanI BanII BbeI BbrPI BbsI
BbvCI BbvI BcnI BglI BlpI
BmyI BpmI Bpu1102I BpuAI BsaAI
BsaHI BsaJI BsaWI BseRI BsgI
BsiEI BsiYI BslI BsmAI BsmBI
BsmFI BsoBI Bsp1286I BspMI BsrBI
BsrFI BsrI BssKI BstF5I BstNI
Cac8I CelII CfoI Cfr10I Cfr13I
DdeI DpnII DraII DsaI EarI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HinfI HpaII
HphI ItaI KasI Ksp632I MaeII
MaeIII MboI MboII MnlI MslI
MspA1I MspI MvaI MwoI NarI
NciI NdeII NlaIII NlaIV NspI
PflMI PleI PmaCI PmlI PpuMI
PstI PvuII RsaI SapI Sau3AI
Sau96I ScrFI SfaNI SfcI SfoI
SmlI StyI TaqI TseI Tsp45I
Tsp509I TspRI TthHB8I Van91I XcmI
XmnI