Restriction map for
pFN21AE6406
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# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AciI AcyI AflII AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI AseI AsnI Asp718I
AspEI AspHI AspI AvaI AviII
AvrII BanI BanII BbeI BbrPI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BmyI BpmI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsiYI
BslI BsmBI BsmFI BsmI BsoBI
Bsp1286I BspDI BspEI BspLU11I BsrBI
BsrDI BsrFI BssHII BssSI Bst1107I
BstAPI BstEII BstPI BstUI BstXI
BstZ17I Bsu36I Cac8I CfoI Cfr10I
ClaI CpoI DraII DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRV EcoT22I FauI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HindIII HpaI HpaII KasI
KpnI KspI MamI MluI MroI
MspI MvnI NaeI NarI NciI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PpuMI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I StuI SwaI Tsp45I Tth111I
Van91I XcmI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AclI 171 AACGTT AA^CGTT
AlwNI 431 CAGACTCTG CAG[ACGT][ACGT][ACGT]^CTG
Asp700I 229 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BalI 345 TGGCCA TGG^CCA
BbsI 1306 GAAGAC GAAGAC(2/6)
BbvI 329 GCTGC GCAGC(8/12)
BclI 561 TGATCA T^GATCA
BlpI 299 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 299 GCTCAGC GC^T[ACGT]AGC
BpuAI 1306 GAAGAC GAAGAC(2/6)
BsaAI 208 CACGTA [CT]AC^GT[GA]
BsaJI 623 CCTTGG C^C[ACGT][ACGT]GG
BsgI 330 CTGCAC GTGCAG(16/14)
BspHI 564 TCATGA T^CATGA
BspMI 177 ACCTGC ACCTGC(4/8)
BsrI 343 ACTGG ACTGG(1/-1)
BstBI 1124 TTCGAA TT^CGAA
CelII 299 GCTCAGC GC^T[ACGT]AGC
DraI 148 TTTAAA TTT^AAA
EaeI 345 TGGCCA [CT]^GGCC[GA]
EarI 1357 GAAGAG CTCTTC(1/4)
EcoT14I 623 CCTTGG C^C[AT][AT]GG
FbaI 561 TGATCA T^GATCA
Fnu4HI 329 GCTGC GC^[ACGT]GC
HincII 25 GTTGAC GT[CT]^[GA]AC
HindII 25 GTTGAC GT[CT]^[GA]AC
ItaI 329 GCTGC GC^[ACGT]GC
Ksp632I 1357 GAAGAG CTCTTC(1/4)
MfeI 542 CAATTG C^AATTG
MluNI 345 TGGCCA TGG^CCA
MscI 345 TGGCCA TGG^CCA
MslI 1203 CACCAAGATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MunI 542 CAATTG C^AATTG
MwoI 703 GCTGTGGTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
NspV 1124 TTCGAA TT^CGAA
Psp1406I 171 AACGTT AA^CGTT
PstI 179 CTGCAG CTGCA^G
RcaI 564 TCATGA T^CATGA
SfuI 1124 TTCGAA TT^CGAA
StyI 623 CCTTGG C^C[AT][AT]GG
TseI 329 GCTGC G^C[AT]GC
XbaI 253 TCTAGA T^CTAGA
XmnI 229 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvaII 398 GGACC G^G[AT]CC
697 GGACC
BamHI 1032 GGATCC G^GATCC
1134 GGATCC
BfaI 254 CTAG C^TAG
874 CTAG
BsmAI 1087 GAGAC GTCTC(1/5)
1271 GAGAC
BssKI 401 CCTGG ^CC[ACGT]GG
945 CCTGG
BstNI 401 CCTGG CC^[AT]GG
945 CCTGG
BstYI 1032 GGATCC [GA]^GATC[CT]
1134 GGATCC
Cfr13I 398 GGACC G^G[ACGT]CC
697 GGACC
EcoRI 280 GAATTC G^AATTC
508 GAATTC
EcoRII 401 CCTGG ^CC[AT]GG
945 CCTGG
HaeIII 346 GGCC GG^CC
404 GGCC
HphI 613 GGTGA GGTGA(8/7)
1202 TCACC
MaeI 254 CTAG C^TAG
874 CTAG
MaeIII 140 GTTAC ^GT[ACGT]AC
174 GTTAC
MflI 1032 GGATCC [GA]^GATC[CT]
1134 GGATCC
MspA1I 701 CAGCTG C[AC]G^C[GT]G
1140 CAGCTG
MvaI 401 CCTGG CC^[AT]GG
945 CCTGG
NlaIV 1032 GGATCC GG[ACGT]^[ACGT]CC
1134 GGATCC
PvuII 701 CAGCTG CAG^CTG
1140 CAGCTG
Sau96I 398 GGACC G^G[ACGT]CC
697 GGACC
ScrFI 401 CCTGG CC^[ACGT]GG
945 CCTGG
SmlI 362 CTCAAG C^T[CT][GA]AG
1084 CTTGAG
TaqI 1125 TCGA T^CGA
1164 TCGA
TfiI 439 GAATC G^A[AT]TC
616 GATTC
TspRI 456 CACTG CA[GC]TG[ACGT][ACGT]^
1096 CACTG
TthHB8I 1125 TCGA T^CGA
1164 TCGA
XhoII 1032 GGATCC [GA]^GATC[CT]
1134 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 28 enzymes) #
#########################################
AcsI AfaI AluI AlwI ApoI
Bsp1407I BsrGI BstF5I DdeI DpnII
FokI HinfI MaeII MboI MboII
MnlI MseI NdeII NlaIII PleI
RsaI Sau3AI SfaNI SfcI SspBI
SspI Tru9I Tsp509I