Restriction map for
pFN21AE1500
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# NO CUT ENZYMES ( 94 enzymes) #
##################################
AatII Acc65I AccI AgeI AhdI
Alw44I Aor51HI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BclI BlnI BsaBI BsaI
BsiWI BsmBI BsmFI Bsp1407I BspDI
BspHI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstEII BstPI BstXI
BstZ17I Cfr10I ClaI CpoI DraIII
DrdI Eam1105I Eco47III EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HgaI HincII HindII HpaI KpnI
KspI MamI MfeI MluI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NsiI PacI PflFI PinAI
PmeI PshAI PshBI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfiI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SwaI
Tth111I XbaI XmaCI XmaI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AccIII 1940 TCCGGA T^CCGGA
AclI 1364 AACGTT AA^CGTT
AcyI 2031 GGCGCC G[GA]^CG[CT]C
AflII 675 CTTAAG C^TTAAG
AocI 1653 CCTGAGG CC^T[ACGT]AGG
ApaI 1719 GGGCCC GGGCC^C
BalI 1439 TGGCCA TGG^CCA
BamHI 71 GGATCC G^GATCC
BanI 2031 GGCGCC G^G[CT][GA]CC
BbeI 2031 GGCGCC GGCGC^C
BbvCI 1707 GCTGAGG CCTCAGC(-5/-2)
BcgI 1769 CGAGGGCCATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BcnI 1998 CCGGG CC^[GC]GG
BfrI 675 CTTAAG C^TTAAG
BglI 352 GCCAAAATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 162 AGATCT A^GATCT
BlpI 979 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 979 GCTGAGC GC^T[ACGT]AGC
BsaHI 2031 GGCGCC G[GA]^CG[CT]C
BsaWI 1940 TCCGGA [AT]^CCGG[AT]
BseAI 1940 TCCGGA T^CCGGA
BsiEI 2266 CGGCCG CG[GA][CT]^CG
BspEI 1940 TCCGGA T^CCGGA
BspLU11I 2097 ACATGT A^CATGT
BspMI 1703 ACCTGC ACCTGC(4/8)
BsrDI 1724 CATTGC GCAATG(2/0)
BstBI 1990 TTCGAA TT^CGAA
Bsu36I 1653 CCTGAGG CC^T[ACGT]AGG
CelII 979 GCTGAGC GC^T[ACGT]AGC
EagI 2266 CGGCCG C^GGCCG
EclXI 2266 CGGCCG C^GGCCG
Eco52I 2266 CGGCCG C^GGCCG
Eco81I 1653 CCTGAGG CC^T[ACGT]AGG
EcoNI 1455 CCTGGAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 2031 GGCGCC G[GA]^CG[CT]C
HindIII 268 AAGCTT A^AGCTT
KasI 2031 GGCGCC G^GCGCC
MluNI 1439 TGGCCA TGG^CCA
MroI 1940 TCCGGA T^CCGGA
MscI 1439 TGGCCA TGG^CCA
NarI 2031 GGCGCC GG^CGCC
NciI 1998 CCGGG CC^[GC]GG
NruI 2124 TCGCGA TCG^CGA
NspV 1990 TTCGAA TT^CGAA
PaeR7I 1767 CTCGAG C^TCGAG
PflMI 1251 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Psp1406I 1364 AACGTT AA^CGTT
SfoI 2031 GGCGCC GGC^GCC
SfuI 1990 TTCGAA TT^CGAA
SspI 439 AATATT AAT^ATT
StuI 1091 AGGCCT AGG^CCT
Van91I 1251 CCAGATAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 1043 CCATTAACACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1767 CTCGAG C^TCGAG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AfaI 632 GTAC GT^AC
2088 GTAC
AflIII 945 ACACGT A^C[GA][CT]GT
2097 ACATGT
AlwNI 175 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
1534 CAGAATCTG
Asp700I 483 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1555 GAAGTGCTTC
AvaI 1061 CTCGGG C^[CT]CG[GA]G
1767 CTCGAG
BbrPI 946 CACGTG CAC^GTG
1759 CACGTG
BbsI 868 GAAGAC GAAGAC(2/6)
1944 GAAGAC
BciVI 996 GGATAC GTATCC(6/5)
2288 GGATAC
BpuAI 868 GAAGAC GAAGAC(2/6)
1944 GAAGAC
BsaAI 946 CACGTG [CT]AC^GT[GA]
1759 CACGTG
BsgI 758 GTGCAG GTGCAG(16/14)
1189 GTGCAG
BsmI 1271 GAATGC GAATGC(1/-1)
1345 GAATGC
BsoBI 1061 CTCGGG C^[CT]CG[GA]G
1767 CTCGAG
BstAPI 1080 GCAGACCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1923 GCAGCGGATGC
DraI 564 TTTAAA TTT^AAA
2106 TTTAAA
EaeI 1439 TGGCCA [CT]^GGCC[GA]
2266 CGGCCG
HaeII 1239 GGCGCT [GA]GCGC^[CT]
2031 GGCGCC
PmaCI 946 CACGTG CAC^GTG
1759 CACGTG
PmlI 946 CACGTG CAC^GTG
1759 CACGTG
RsaI 632 GTAC GT^AC
2088 GTAC
SapI 721 GAAGAGC GCTCTTC(1/4)
1986 GCTCTTC
TaqI 1768 TCGA T^CGA
1991 TCGA
TthHB8I 1768 TCGA T^CGA
1991 TCGA
XmnI 483 GAATCTTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1555 GAAGTGCTTC
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# 3 OR MORE CUT ENZYMES ( 86 enzymes) #
#########################################
AccII AciI AcsI AluI AlwI
ApoI AspHI AvaII BanII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiHKAI BsiYI BslI BsmAI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
BstYI Cac8I CfoI Cfr13I DdeI
DpnII DraII DsaI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MnlI MseI MspA1I MspI MvaI
MvnI MwoI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI PstI
PvuII SacI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
XhoII