Restriction map for
pFN21AE3593
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# NO CUT ENZYMES ( 181 enzymes) #
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AatII Acc65I AccI AccII AclI
AcyI AfaI AflII AflIII AgeI
AhdI Alw44I AlwNI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaI
AvrII BalI BamHI BanI BbeI
BbrPI BbsI BbvCI BbvI BcgI
BcnI BfaI BfrI BglI BlnI
BlpI Bpu1102I BpuAI BsaAI BsaBI
BsaHI BsaI BsaJI BsgI BsiEI
BsiHKAI BsiWI BsmAI BsmBI BsmFI
BsoBI Bsp1407I BspDI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstUI BstZ17I Bsu36I CelII ClaI
CpoI DdeI DraI DraIII DrdI
DsaI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoRV EcoT14I EcoT22I Fnu4HI
FseI HaeII HgaI Hin1I HincII
HindII HindIII HpaI ItaI KasI
KpnI KspI MaeI MaeIII MamI
MfeI MluI MluNI MscI MseI
MspA1I MunI MvnI MwoI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflFI PinAI PleI
PmaCI PmeI PmlI PpuMI PshAI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SnaBI SpeI Sse8387I
SspBI SspI StuI StyI SwaI
TaqI Tru9I TseI Tsp45I Tth111I
TthHB8I XbaI XcmI XhoI XmaCI
XmaI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AccIII 396 TCCGGA T^CCGGA
AcsI 205 AAATTC [GA]^AATT[CT]
AlwI 36 GGATC GGATC(4/5)
ApoI 205 AAATTC [GA]^AATT[CT]
Asp700I 144 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AviII 158 TGCGCA TGC^GCA
BanII 355 GAGCCC G[GA]GC[CT]^C
BciVI 180 GTATCC GTATCC(6/5)
BclI 200 TGATCA T^GATCA
BglII 218 AGATCT A^GATCT
BmyI 355 GAGCCC G[AGT]GC[ACT]^C
BsaWI 396 TCCGGA [AT]^CCGG[AT]
BseAI 396 TCCGGA T^CCGGA
BseRI 112 CTCCTC GAGGAG(10/8)
BsmI 414 GAATGC GAATGC(1/-1)
Bsp1286I 355 GAGCCC G[AGT]GC[ACT]^C
BspEI 396 TCCGGA T^CCGGA
BsrFI 449 ACCGGC [GA]^CCGG[CT]
BsrI 281 ACTGG ACTGG(1/-1)
BstF5I 365 GGATG GGATG(2/0)
BstXI 89 CCAATGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 159 GCGC GCG^C
Cfr10I 449 ACCGGC [GA]^CCGG[CT]
DraII 317 AGGCCCT [GA]G^G[ACGT]CC[CT]
EarI 421 GAAGAG CTCTTC(1/4)
EcoO109I 317 AGGCCCT [GA]G^G[ACGT]CC[CT]
FauI 171 CCCGC CCCGC(4/6)
FbaI 200 TGATCA T^GATCA
FokI 365 GGATG GGATG(9/13)
FspI 158 TGCGCA TGC^GCA
HhaI 159 GCGC GCG^C
HinP1I 159 GCGC G^CGC
Ksp632I 421 GAAGAG CTCTTC(1/4)
MaeII 233 ACGT A^CGT
MroI 396 TCCGGA T^CCGGA
MslI 12 CATGCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NspI 11 GCATGC [GA]CATG^[CT]
PflMI 306 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
SphI 11 GCATGC GCATG^C
Tsp509I 206 AATT ^AATT
TspRI 273 CAGTG CA[GC]TG[ACGT][ACGT]^
Van91I 306 CCAAGTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmnI 144 GAAAGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AvaII 369 GGACC G^G[AT]CC
447 GGACC
BpmI 31 CTGGAG CTGGAG(16/14)
313 CTGGAG
BssKI 96 CCTGG ^CC[ACGT]GG
312 CCTGG
BstNI 96 CCTGG CC^[AT]GG
312 CCTGG
BstYI 36 GGATCT [GA]^GATC[CT]
218 AGATCT
EcoRII 96 CCTGG ^CC[AT]GG
312 CCTGG
HaeIII 318 GGCC GG^CC
452 GGCC
HinfI 393 GAATC G^A[ACGT]TC
437 GATTC
MflI 36 GGATCT [GA]^GATC[CT]
218 AGATCT
MvaI 96 CCTGG CC^[AT]GG
312 CCTGG
NlaIII 12 CATG CATG^
198 CATG
NlaIV 68 GGAACC GG[ACGT]^[ACGT]CC
354 GGAGCC
ScrFI 96 CCTGG CC^[ACGT]GG
312 CCTGG
SfaNI 85 GATGC GCATC(5/9)
161 GCATC
TfiI 393 GAATC G^A[AT]TC
437 GATTC
XhoII 36 GGATCT [GA]^GATC[CT]
218 AGATCT
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# 3 OR MORE CUT ENZYMES ( 18 enzymes) #
#########################################
AciI AluI BsiYI BslI BspMI
Cac8I Cfr13I DpnII HapII HpaII
HphI MboI MboII MnlI MspI
NdeII Sau3AI Sau96I