Restriction map for
pFN21AE4452
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# NO CUT ENZYMES ( 114 enzymes) #
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Acc65I AccIII AclI AcsI AgeI
AhdI Alw44I Aor51HI ApaLI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AspI AviII AvrII BalI
BamHI BbrPI BcgI BciVI BglII
BlnI BsaAI BsaBI BseAI BsgI
BsiEI BsiWI BsmBI BsmI Bsp1407I
BspDI BspEI BspHI BsrGI BssSI
Bst1107I BstBI BstEII BstPI BstZ17I
ClaI CpoI DraI DraIII DrdI
EagI Eam1105I EclXI Eco47III Eco52I
EcoO65I EcoRI EcoT14I EcoT22I FseI
FspI HindIII HpaI KpnI MamI
MfeI MluI MluNI MroI MscI
MslI MunI NcoI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI PvuII RcaI RsrII
SapI SbfI ScaI SfiI SfuI
SgrAI SnaBI SpeI SphI Sse8387I
SspBI SspI StyI SwaI Tth111I
Van91I XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 52 enzymes) #
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AccI 526 GTCGAC GT^[AC][GT]AC
AflII 715 CTTAAG C^TTAAG
AflIII 791 ACATGT A^C[GA][CT]GT
AlwNI 1165 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 819 CCTCAGG CC^T[ACGT]AGG
ApaI 59 GGGCCC GGGCC^C
AvaI 63 CCCGGG C^[CT]CG[GA]G
BbeI 488 GGCGCC GGCGC^C
BbsI 1329 GAAGAC GAAGAC(2/6)
BbvCI 874 GCTGAGG CCTCAGC(-5/-2)
BclI 1340 TGATCA T^GATCA
BfaI 658 CTAG C^TAG
BfrI 715 CTTAAG C^TTAAG
BglI 1069 GCCAGTGAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 1329 GAAGAC GAAGAC(2/6)
BsaI 844 GAGACC GGTCTC(1/5)
BsaWI 1214 ACCGGA [AT]^CCGG[AT]
BsoBI 63 CCCGGG C^[CT]CG[GA]G
BspLU11I 791 ACATGT A^CATGT
BsrDI 1098 CATTGC GCAATG(2/0)
BstAPI 1398 GCATGAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 212 CCAGCCGGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 819 CCTCAGG CC^T[ACGT]AGG
DsaI 106 CCGCGG C^C[GA][CT]GG
EarI 730 GAAGAG CTCTTC(1/4)
Eco81I 819 CCTCAGG CC^T[ACGT]AGG
EcoRV 826 GATATC GAT^ATC
FbaI 1340 TGATCA T^GATCA
HincII 526 GTCGAC GT[CT]^[GA]AC
HindII 526 GTCGAC GT[CT]^[GA]AC
KasI 488 GGCGCC G^GCGCC
Ksp632I 730 GAAGAG CTCTTC(1/4)
KspI 106 CCGCGG CCGC^GG
MaeI 658 CTAG C^TAG
MaeIII 145 GTGAC ^GT[ACGT]AC
MseI 716 TTAA T^TAA
NaeI 215 GCCGGC GCC^GGC
NarI 488 GGCGCC GG^CGCC
NgoMIV 215 GCCGGC G^CCGGC
NspI 791 ACATGT [GA]CATG^[CT]
PpuMI 1166 AGGACCT [GA]G^G[AT]CC[CT]
SacII 106 CCGCGG CCGC^GG
SalI 526 GTCGAC G^TCGAC
SexAI 161 ACCAGGT A^CC[AT]GGT
SfoI 488 GGCGCC GGC^GCC
SmaI 63 CCCGGG CCC^GGG
StuI 8 AGGCCT AGG^CCT
Tru9I 716 TTAA T^TAA
Tsp45I 145 GTGAC ^GT[GC]AC
XcmI 317 CCAACGAGAAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmaCI 63 CCCGGG C^CCGGG
XmaI 63 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AatII 147 GACGTC GACGT^C
529 GACGTC
BanI 488 GGCGCC G^G[CT][GA]CC
1124 GGCACC
BlpI 87 GCTGAGC GC^T[ACGT]AGC
754 GCTCAGC
Bpu1102I 87 GCTGAGC GC^T[ACGT]AGC
754 GCTCAGC
BsmAI 844 GAGAC GTCTC(1/5)
1354 GAGAC
BsmFI 223 GGGAC GGGAC(10/14)
150 GTCCC
BsrBI 73 CCGCTC CCGCTC(-3/-3)
635 GAGCGG
BsrFI 215 GCCGGC [GA]^CCGG[CT]
422 ACCGGC
BstYI 737 AGATCC [GA]^GATC[CT]
980 AGATCC
CelII 87 GCTGAGC GC^T[ACGT]AGC
754 GCTCAGC
Cfr10I 215 GCCGGC [GA]^CCGG[CT]
422 ACCGGC
EaeI 278 TGGCCG [CT]^GGCC[GA]
452 TGGCCG
HaeII 402 GGCGCT [GA]GCGC^[CT]
488 GGCGCC
MflI 737 AGATCC [GA]^GATC[CT]
980 AGATCC
SfaNI 646 GATGC GCATC(5/9)
1044 GATGC
XhoII 737 AGATCC [GA]^GATC[CT]
980 AGATCC
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# 3 OR MORE CUT ENZYMES ( 76 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AspHI AvaII BanII BbvI
BcnI BmyI BpmI BsaHI BsaJI
BseRI BsiHKAI BsiYI BslI Bsp1286I
BspMI BsrI BssHII BssKI BstF5I
BstNI BstUI Cac8I CfoI Cfr13I
DdeI DpnII DraII EcoNI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI Hin1I HinP1I
HinfI HpaII HphI ItaI MaeII
MboI MboII MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PstI RsaI SacI
Sau3AI Sau96I ScrFI SfcI SmlI
TaqI TfiI TseI Tsp509I TspRI
TthHB8I