Restriction map for
pFN21AE6407
##################################
# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AflII AgeI AhdI Alw44I
AocI Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AspI AviII AvrII BamHI BbrPI
BbvCI BcgI BclI BcnI BfaI
BfrI BlnI BlpI BpmI Bpu1102I
BsaAI BsaBI BsaWI BseAI BsiEI
BsiWI BsmBI BspDI BspEI BssHII
BssSI Bst1107I BstBI BstUI BstXI
BstZ17I Bsu36I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoNI
EcoRI EcoRV FauI FbaI FseI
FspI HindIII HpaI KpnI KspI
MaeI MamI MfeI MluI MroI
MunI MvnI NaeI NciI NcoI
NdeI NgoMIV NheI NotI NruI
NspV PacI PaeR7I PflFI PinAI
PleI PmaCI PmeI PmlI PshAI
PshBI Psp1406I PvuI RsrII SacI
SacII SalI SbfI ScaI SfaNI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspI
StuI SwaI TaqI Tth111I TthHB8I
XbaI XcmI XhoI XmaCI XmaI
XmnI
###################################
# ONE CUT ENZYMES ( 64 enzymes) #
###################################
AcsI 1352 GAATTT [GA]^AATT[CT]
AcyI 918 GGCGCC G[GA]^CG[CT]C
AflIII 497 ACATGT A^C[GA][CT]GT
AlwI 51 GATCC GGATC(4/5)
AlwNI 27 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 1352 GAATTT [GA]^AATT[CT]
AspHI 400 GAGCAC G[AT]GC[AT]^C
AvaI 397 CCCGAG C^[CT]CG[GA]G
BalI 297 TGGCCA TGG^CCA
BanI 918 GGCGCC G^G[CT][GA]CC
BbeI 918 GGCGCC GGCGC^C
BciVI 484 GTATCC GTATCC(6/5)
BglI 355 GCCGAGGTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 161 AGATCT A^GATCT
BsaHI 918 GGCGCC G[GA]^CG[CT]C
BseRI 819 CTCCTC GAGGAG(10/8)
BsiHKAI 400 GAGCAC G[AT]GC[AT]^C
BsmFI 1022 GGGAC GGGAC(10/14)
BsmI 1307 GCATTC GAATGC(1/-1)
BsoBI 397 CCCGAG C^[CT]CG[GA]G
Bsp1407I 1407 TGTACA T^GTACA
BspHI 1259 TCATGA T^CATGA
BspLU11I 497 ACATGT A^CATGT
BsrBI 417 GAGCGG CCGCTC(-3/-3)
BsrDI 764 GCAATG GCAATG(2/0)
BsrFI 1069 GCCGGT [GA]^CCGG[CT]
BsrGI 1407 TGTACA T^GTACA
BstAPI 1339 GCAGTAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 345 GGTCACC G^GT[ACGT]ACC
BstPI 345 GGTCACC G^GT[ACGT]ACC
BstYI 161 AGATCT [GA]^GATC[CT]
CfoI 919 GCGC GCG^C
Cfr10I 1069 GCCGGT [GA]^CCGG[CT]
DraII 1022 GGGACCT [GA]G^G[ACGT]CC[CT]
DsaI 542 CCGTGG C^C[GA][CT]GG
EcoO109I 1022 GGGACCT [GA]G^G[ACGT]CC[CT]
EcoO65I 345 GGTCACC G^GT[ACGT]ACC
EcoT22I 1087 ATGCAT ATGCA^T
HaeII 918 GGCGCC [GA]GCGC^[CT]
HapII 1070 CCGG C^CGG
HgaI 434 GCGTC GACGC(5/10)
HhaI 919 GCGC GCG^C
Hin1I 918 GGCGCC G[GA]^CG[CT]C
HinP1I 919 GCGC G^CGC
HpaII 1070 CCGG C^CGG
KasI 918 GGCGCC G^GCGCC
MflI 161 AGATCT [GA]^GATC[CT]
MluNI 297 TGGCCA TGG^CCA
MscI 297 TGGCCA TGG^CCA
MspA1I 742 CAGCTG C[AC]G^C[GT]G
MspI 1070 CCGG C^CGG
NarI 918 GGCGCC GG^CGCC
NsiI 1087 ATGCAT ATGCA^T
PflMI 1100 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 1022 GGGACCT [GA]G^G[AT]CC[CT]
PstI 426 CTGCAG CTGCA^G
PvuII 742 CAGCTG CAG^CTG
RcaI 1259 TCATGA T^CATGA
SapI 685 GAAGAGC GCTCTTC(1/4)
SfcI 426 CTGCAG C^T[GA][CT]AG
SfoI 918 GGCGCC GGC^GCC
SspBI 1407 TGTACA T^GTACA
Van91I 1100 CCAATATCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 161 AGATCT [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AfaI 504 GTAC GT^AC
1408 GTAC
AvaII 121 GGACC G^G[AT]CC
1023 GGACC
BanII 1288 GGGCTC G[GA]GC[CT]^C
1413 GAGCCC
BbsI 309 GAAGAC GAAGAC(2/6)
1051 GTCTTC
BpuAI 309 GAAGAC GAAGAC(2/6)
1051 GTCTTC
BsaI 84 GAGACC GGTCTC(1/5)
842 GGTCTC
BsgI 143 GTGCAG GTGCAG(16/14)
787 GTGCAG
BsiYI 971 CCTTTGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1100 CCAATATCTGG
BslI 971 CCTTTGGAGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
1100 CCAATATCTGG
BspMI 318 GCAGGT ACCTGC(4/8)
868 GCAGGT
EaeI 297 TGGCCA [CT]^GGCC[GA]
443 TGGCCG
EarI 685 GAAGAG CTCTTC(1/4)
1456 GAAGAG
HincII 1015 GTCAAC GT[CT]^[GA]AC
1476 GTCAAC
HindII 1015 GTCAAC GT[CT]^[GA]AC
1476 GTCAAC
Ksp632I 685 GAAGAG CTCTTC(1/4)
1456 GAAGAG
MseI 875 TTAA T^TAA
1187 TTAA
MslI 661 CACGAAGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1260 CATGACTGTG
RsaI 504 GTAC GT^AC
1408 GTAC
SexAI 188 ACCTGGT A^CC[AT]GGT
878 ACCAGGT
Tru9I 875 TTAA T^TAA
1187 TTAA
Tsp509I 1344 AATT ^AATT
1353 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 42 enzymes) #
#########################################
AciI AluI BbvI BmyI BsaJI
BsmAI Bsp1286I BsrI BssKI BstF5I
BstNI Cac8I Cfr13I DdeI DpnII
EcoRII EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI MaeII MaeIII
MboI MboII MnlI MvaI MwoI
NdeII NlaIII NlaIV NspI Sau3AI
Sau96I ScrFI StyI TfiI TseI
Tsp45I TspRI