Restriction map for
pFN21AE2352
##################################
# NO CUT ENZYMES ( 160 enzymes) #
##################################
AatII Acc65I AccIII AclI AcyI
AfaI AflII AflIII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BamHI BanI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglI
BglII BlnI BlpI Bpu1102I BsaAI
BsaBI BsaHI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrFI
BsrGI BssHII BssSI BstAPI BstBI
BstEII BstPI BstXI BstYI Bsu36I
Cac8I CelII Cfr10I ClaI CpoI
DraI DraII DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO109I EcoO65I EcoRV EcoT22I
FbaI FseI FspI HaeII HapII
HgaI Hin1I HincII HindII HpaI
HpaII KasI KpnI Ksp632I KspI
MamI MfeI MflI MluI MroI
MseI MspI MunI NaeI NarI
NciI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI SphI Sse8387I SspBI
SspI SwaI TaqI Tru9I TthHB8I
XhoI XhoII XmaCI XmaI XmnI
###################################
# ONE CUT ENZYMES ( 44 enzymes) #
###################################
AccI 215 GTATAC GT^[AC][GT]AC
AccII 739 CGCG CG^CG
AcsI 242 GAATTC [GA]^AATT[CT]
AlwNI 407 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 242 GAATTC [GA]^AATT[CT]
AspHI 442 GTGCTC G[AT]GC[AT]^C
AspI 817 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 562 CCCGAG C^[CT]CG[GA]G
AvaII 161 GGTCC G^G[AT]CC
BbsI 518 GTCTTC GAAGAC(2/6)
BfaI 805 CTAG C^TAG
BpuAI 518 GTCTTC GAAGAC(2/6)
BseRI 184 GAGGAG GAGGAG(10/8)
BsgI 313 CTGCAC GTGCAG(16/14)
BsiHKAI 442 GTGCTC G[AT]GC[AT]^C
BsoBI 562 CCCGAG C^[CT]CG[GA]G
BspMI 496 ACCTGC ACCTGC(4/8)
BsrDI 179 GCAATG GCAATG(2/0)
Bst1107I 215 GTATAC GTA^TAC
BstUI 739 CGCG CG^CG
BstZ17I 215 GTATAC GTA^TAC
EcoNI 158 CCTGGTCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoRI 242 GAATTC G^AATTC
EcoT14I 579 CCATGG C^C[AT][AT]GG
FauI 666 GCGGG CCCGC(4/6)
HindIII 336 AAGCTT A^AGCTT
MaeI 805 CTAG C^TAG
MaeII 504 ACGT A^CGT
MslI 460 CATGAAAATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MvnI 739 CGCG CG^CG
NcoI 579 CCATGG C^CATGG
PflFI 817 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 406 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 111 GACTC GAGTC(4/5)
PstI 607 CTGCAG CTGCA^G
PvuII 770 CAGCTG CAG^CTG
SfcI 607 CTGCAG C^T[GA][CT]AG
SmlI 13 CTCAAG C^T[CT][GA]AG
StuI 566 AGGCCT AGG^CCT
StyI 579 CCATGG C^C[AT][AT]GG
Tth111I 817 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 406 CCAGTGGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 804 TCTAGA T^CTAGA
XcmI 640 CCAAAGCCCGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AlwI 44 GGATC GGATC(4/5)
361 GGATC
BalI 422 TGGCCA TGG^CCA
600 TGGCCA
BmyI 442 GTGCTC G[AGT]GC[ACT]^C
687 GGGCAC
BpmI 544 CTCCAG CTGGAG(16/14)
748 CTGGAG
BsaI 84 GGTCTC GGTCTC(1/5)
327 GGTCTC
Bsp1286I 442 GTGCTC G[AGT]GC[ACT]^C
687 GGGCAC
BstF5I 50 CATCC GGATG(2/0)
662 GGATG
CfoI 740 GCGC GCG^C
904 GCGC
DpnII 45 GATC ^GATC
362 GATC
DsaI 579 CCATGG C^C[GA][CT]GG
848 CCGTGG
FokI 50 CATCC GGATG(9/13)
662 GGATG
HhaI 740 GCGC GCG^C
904 GCGC
HinP1I 740 GCGC G^CGC
904 GCGC
MaeIII 295 GTGAC ^GT[ACGT]AC
491 GTGAC
MboI 45 GATC ^GATC
362 GATC
MluNI 422 TGGCCA TGG^CCA
600 TGGCCA
MscI 422 TGGCCA TGG^CCA
600 TGGCCA
NdeII 45 GATC ^GATC
362 GATC
NlaIV 18 GGAGCC GG[ACGT]^[ACGT]CC
583 GGCCCC
Sau3AI 45 GATC ^GATC
362 GATC
TfiI 453 GAATC G^A[AT]TC
632 GATTC
Tsp45I 295 GTGAC ^GT[GC]AC
491 GTGAC
#########################################
# 3 OR MORE CUT ENZYMES ( 32 enzymes) #
#########################################
AciI AluI BbvI BsaJI BsiYI
BslI BsmAI BsmFI BsrI BssKI
BstNI Cfr13I DdeI EaeI EcoRII
Fnu4HI HaeIII HinfI HphI ItaI
MboII MnlI MspA1I MvaI MwoI
NlaIII Sau96I ScrFI SfaNI TseI
Tsp509I TspRI