Restriction map for
pFN21AE1552
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# NO CUT ENZYMES ( 133 enzymes) #
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AatII Acc65I AccII AccIII AclI
AflII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp718I AspEI AspI AvaI
AviII AvrII BalI BamHI BanII
BbeI BbrPI BbvCI BcgI BciVI
BclI BfrI BglI BglII BlnI
BsaI BsaWI BseAI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspEI BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstUI BstZ17I Bsu36I Cfr10I
ClaI CpoI DraI EagI Eam1105I
EclXI Eco47III Eco52I Eco81I EcoO65I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HpaI KasI KpnI KspI
MfeI MluI MluNI MroI MscI
MunI MvnI NaeI NarI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SpeI Sse8387I SspBI SspI
StuI SwaI TaqI Tth111I TthHB8I
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 1615 GTCTAC GT^[AC][GT]AC
AcyI 984 GGCGTC G[GA]^CG[CT]C
AflIII 686 ACATGT A^C[GA][CT]GT
BanI 1555 GGCACC G^G[CT][GA]CC
BbsI 1293 GAAGAC GAAGAC(2/6)
BlpI 827 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 827 GCTCAGC GC^T[ACGT]AGC
BpuAI 1293 GAAGAC GAAGAC(2/6)
BsaBI 142 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 984 GGCGTC G[GA]^CG[CT]C
BseRI 664 CTCCTC GAGGAG(10/8)
BspHI 1155 TCATGA T^CATGA
BspLU11I 686 ACATGT A^CATGT
BsrDI 1238 CATTGC GCAATG(2/0)
BstYI 511 AGATCC [GA]^GATC[CT]
CelII 827 GCTCAGC GC^T[ACGT]AGC
CfoI 1048 GCGC GCG^C
DraIII 1422 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 259 GACTGCCTCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 360 TGGCCG [CT]^GGCC[GA]
HaeII 1047 GGCGCT [GA]GCGC^[CT]
HgaI 985 GCGTC GACGC(5/10)
HhaI 1048 GCGC GCG^C
Hin1I 984 GGCGTC G[GA]^CG[CT]C
HinP1I 1048 GCGC G^CGC
HincII 628 GTCAAC GT[CT]^[GA]AC
HindII 628 GTCAAC GT[CT]^[GA]AC
HindIII 880 AAGCTT A^AGCTT
MamI 142 GATTATAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 511 AGATCC [GA]^GATC[CT]
PstI 735 CTGCAG CTGCA^G
RcaI 1155 TCATGA T^CATGA
SfcI 735 CTGCAG C^T[GA][CT]AG
SmlI 1243 CTCAAG C^T[CT][GA]AG
SphI 636 GCATGC GCATG^C
XbaI 1275 TCTAGA T^CTAGA
XhoII 511 AGATCC [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 19 enzymes) #
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AfaI 201 GTAC GT^AC
1191 GTAC
AlwNI 1132 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
1387 CAGCTCCTG
Asp700I 1147 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1179 GAAGATATTC
BfaI 1123 CTAG C^TAG
1276 CTAG
BsaAI 339 TACGTG [CT]AC^GT[GA]
1288 TACGTG
BsgI 1086 CTGCAC GTGCAG(16/14)
1478 GTGCAG
BsmAI 374 GTCTC GTCTC(1/5)
817 GTCTC
BspMI 490 ACCTGC ACCTGC(4/8)
1510 ACCTGC
EarI 154 GAAGAG CTCTTC(1/4)
1602 GAAGAG
EcoNI 421 CCTTACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
666 CCTCTTGCAGG
Ksp632I 154 GAAGAG CTCTTC(1/4)
1602 GAAGAG
MaeI 1123 CTAG C^TAG
1276 CTAG
PflMI 1255 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1386 CCAGCTCCTGG
PvuII 291 CAGCTG CAG^CTG
435 CAGCTG
RsaI 201 GTAC GT^AC
1191 GTAC
Tsp45I 1265 GTCAC ^GT[GC]AC
1520 GTGAC
Van91I 1255 CCAGTGGATGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1386 CCAGCTCCTGG
XcmI 1032 CCACTTCTGTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1515 CCAGGGTGACTCTGG
XmnI 1147 GAAGAATTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1179 GAAGATATTC
#########################################
# 3 OR MORE CUT ENZYMES ( 69 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
AspHI AvaII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmFI Bsp1286I BsrI BssKI BstF5I
BstNI BstXI Cac8I Cfr13I DdeI
DpnII DraII DsaI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HinfI HpaII HphI ItaI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV NspI PleI PpuMI Sau3AI
Sau96I ScrFI SfaNI StyI TfiI
Tru9I TseI Tsp509I TspRI