Restriction map for
pFN21AE3610
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# NO CUT ENZYMES ( 125 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI AocI Aor51HI ApaLI AscI
AseI AsnI Asp718I AspEI AspHI
AviII AvrII BamHI BbrPI BbvCI
BfaI BfrI BglI BglII BlnI
BlpI Bpu1102I BsaBI BsaWI BseAI
BsgI BsiEI BsiHKAI BsiWI BsmBI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I Bsu36I CelII
Cfr10I ClaI CpoI DraI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoO65I EcoRI EcoRV FseI
FspI HpaI KpnI Ksp632I MaeI
MamI MfeI MluI MroI MunI
MwoI NaeI NdeI NgoMIV NheI
NotI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SalI SapI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SpeI
Sse8387I SspBI SspI StuI SwaI
TfiI XbaI XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 61 enzymes) #
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AfaI 244 GTAC GT^AC
ApaI 394 GGGCCC GGGCC^C
AspI 1036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 200 CCCGAG C^[CT]CG[GA]G
BalI 520 TGGCCA TGG^CCA
BanI 111 GGCGCC G^G[CT][GA]CC
BanII 394 GGGCCC G[GA]GC[CT]^C
BbeI 111 GGCGCC GGCGC^C
BcgI 202 CGAGACTTATGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BciVI 986 GTATCC GTATCC(6/5)
BclI 720 TGATCA T^GATCA
BmyI 394 GGGCCC G[AGT]GC[ACT]^C
BpmI 604 CTGGAG CTGGAG(16/14)
BsaAI 214 TACGTA [CT]AC^GT[GA]
BsaI 195 GGTCTC GGTCTC(1/5)
BseRI 322 CTCCTC GAGGAG(10/8)
BsoBI 200 CCCGAG C^[CT]CG[GA]G
Bsp1286I 394 GGGCCC G[AGT]GC[ACT]^C
BspMI 545 ACCTGC ACCTGC(4/8)
BsrDI 846 CATTGC GCAATG(2/0)
BstYI 968 AGATCC [GA]^GATC[CT]
CfoI 112 GCGC GCG^C
DraIII 524 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 1063 GACATGGTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 520 TGGCCA [CT]^GGCC[GA]
EcoT22I 949 ATGCAT ATGCA^T
FauI 236 GCGGG CCCGC(4/6)
FbaI 720 TGATCA T^GATCA
HaeII 111 GGCGCC [GA]GCGC^[CT]
HgaI 230 GCGTC GACGC(5/10)
HhaI 112 GCGC GCG^C
HinP1I 112 GCGC G^CGC
KasI 111 GGCGCC G^GCGCC
KspI 234 CCGCGG CCGC^GG
MaeII 215 ACGT A^CGT
MflI 968 AGATCC [GA]^GATC[CT]
MluNI 520 TGGCCA TGG^CCA
MscI 520 TGGCCA TGG^CCA
MslI 1008 CATTTCCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NarI 111 GGCGCC GG^CGCC
NruI 171 TCGCGA TCG^CGA
NsiI 949 ATGCAT ATGCA^T
NspI 951 GCATGC [GA]CATG^[CT]
PflFI 1036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 90 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 821 GGGACCT [GA]G^G[AT]CC[CT]
PstI 313 CTGCAG CTGCA^G
PvuII 376 CAGCTG CAG^CTG
RsaI 244 GTAC GT^AC
SacII 234 CCGCGG CCGC^GG
SfcI 313 CTGCAG C^T[GA][CT]AG
SfoI 111 GGCGCC GGC^GCC
SmlI 633 CTCAAG C^T[CT][GA]AG
SnaBI 214 TACGTA TAC^GTA
SphI 951 GCATGC GCATG^C
TaqI 1073 TCGA T^CGA
Tth111I 1036 GACAATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 1073 TCGA T^CGA
Van91I 90 CCAATTTCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 854 CCAAGGGGTTCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 968 AGATCC [GA]^GATC[CT]
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AccII 172 CGCG CG^CG
235 CGCG
AcyI 111 GGCGCC G[GA]^CG[CT]C
229 GGCGTC
Asp700I 156 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
585 GAAAAATTTC
AvaII 418 GGACC G^G[AT]CC
822 GGACC
BbsI 655 GAAGAC GAAGAC(2/6)
1060 GAAGAC
BcnI 581 CCCGG CC^[GC]GG
819 CCGGG
BpuAI 655 GAAGAC GAAGAC(2/6)
1060 GAAGAC
BsaHI 111 GGCGCC G[GA]^CG[CT]C
229 GGCGTC
BstF5I 756 CATCC GGATG(2/0)
552 GGATG
BstUI 172 CGCG CG^CG
235 CGCG
DraII 393 GGGGCCC [GA]G^G[ACGT]CC[CT]
821 GGGACCT
EcoNI 444 CCTGGTTCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
481 CCTAAGCCAGG
EcoO109I 393 GGGGCCC [GA]G^G[ACGT]CC[CT]
821 GGGACCT
FokI 756 CATCC GGATG(9/13)
552 GGATG
HapII 582 CCGG C^CGG
819 CCGG
Hin1I 111 GGCGCC G[GA]^CG[CT]C
229 GGCGTC
HincII 363 GTTGAC GT[CT]^[GA]AC
958 GTTGAC
HindII 363 GTTGAC GT[CT]^[GA]AC
958 GTTGAC
HindIII 299 AAGCTT A^AGCTT
769 AAGCTT
HinfI 631 GACTC G^A[ACGT]TC
708 GACTC
HpaII 582 CCGG C^CGG
819 CCGG
MaeIII 706 GTGAC ^GT[ACGT]AC
797 GTCAC
MspA1I 234 CCGCGG C[AC]G^C[GT]G
376 CAGCTG
MspI 582 CCGG C^CGG
819 CCGG
MvnI 172 CGCG CG^CG
235 CGCG
NciI 581 CCCGG CC^[GC]GG
819 CCGGG
NcoI 517 CCATGG C^CATGG
879 CCATGG
PleI 631 GACTC GAGTC(4/5)
708 GACTC
SfaNI 677 GATGC GCATC(5/9)
948 GATGC
Tsp45I 706 GTGAC ^GT[GC]AC
797 GTCAC
XmnI 156 GAAAATTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
585 GAAAAATTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 41 enzymes) #
#########################################
AciI AcsI AluI AlwI ApoI
BbvI BsaJI BsiYI BslI BsmAI
BsmFI BsrI BssKI BstNI Cac8I
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI HaeIII HphI ItaI
MboI MboII MnlI MseI MvaI
NdeII NlaIII NlaIV Sau3AI Sau96I
ScrFI StyI Tru9I TseI Tsp509I
TspRI