Restriction map for
pFN21AE3518
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# NO CUT ENZYMES ( 114 enzymes) #
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Acc65I AccI AccII AccIII AgeI
Alw44I Aor51HI ApaI ApaLI AscI
AseI AsnI Asp700I Asp718I AspI
AvaI AviII BalI BamHI BbrPI
BbvCI BcnI BglII BsaAI BsaBI
BsaWI BseAI BsiEI BsiWI BsmBI
BsmI BsoBI BspDI BspEI BspLU11I
BspMI BsrFI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Cfr10I ClaI CpoI DraII
DraIII DrdI EaeI EagI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRV FseI FspI HapII HgaI
HincII HindII HpaI HpaII KpnI
KspI MamI MluI MluNI MroI
MscI MspI MvnI NaeI NciI
NgoMIV NheI NotI NruI NspV
PacI PaeR7I PflFI PinAI PmaCI
PmeI PmlI PpuMI PshBI PvuI
RsrII SacII SalI ScaI SexAI
SfiI SfuI SgrAI SmaI SnaBI
SphI SwaI Tth111I XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 54 enzymes) #
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AatII 25 GACGTC GACGT^C
AflII 439 CTTAAG C^TTAAG
AflIII 1791 ACGTGT A^C[GA][CT]GT
AhdI 1120 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1083 GGATC GGATC(4/5)
AlwNI 1106 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 628 CCTGAGG CC^T[ACGT]AGG
AspEI 1120 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvrII 1735 CCTAGG C^CTAGG
BanI 3 GGCGCC G^G[CT][GA]CC
BbeI 3 GGCGCC GGCGC^C
BciVI 486 GGATAC GTATCC(6/5)
BclI 1776 TGATCA T^GATCA
BfrI 439 CTTAAG C^TTAAG
BglI 2060 GCCGCTCTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BlnI 1735 CCTAGG C^CTAGG
BlpI 264 GCTTAGC GC^T[ACGT]AGC
Bpu1102I 264 GCTTAGC GC^T[ACGT]AGC
BsgI 1922 GTGCAG GTGCAG(16/14)
BspHI 855 TCATGA T^CATGA
BsrBI 2061 CCGCTC CCGCTC(-3/-3)
BstYI 1083 GGATCT [GA]^GATC[CT]
Bsu36I 628 CCTGAGG CC^T[ACGT]AGG
CelII 264 GCTTAGC GC^T[ACGT]AGC
CfoI 4 GCGC GCG^C
DraI 550 TTTAAA TTT^AAA
Eam1105I 1120 GACAGTTGGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 628 CCTGAGG CC^T[ACGT]AGG
EcoRI 1226 GAATTC G^AATTC
EcoT22I 166 ATGCAT ATGCA^T
FauI 784 CCCGC CCCGC(4/6)
FbaI 1776 TGATCA T^GATCA
HaeII 3 GGCGCC [GA]GCGC^[CT]
HhaI 4 GCGC GCG^C
HinP1I 4 GCGC G^CGC
HindIII 82 AAGCTT A^AGCTT
KasI 3 GGCGCC G^GCGCC
MfeI 911 CAATTG C^AATTG
MflI 1083 GGATCT [GA]^GATC[CT]
MunI 911 CAATTG C^AATTG
NarI 3 GGCGCC GG^CGCC
NdeI 977 CATATG CA^TATG
NsiI 166 ATGCAT ATGCA^T
NspI 1948 GCATGT [GA]CATG^[CT]
PflMI 976 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 855 TCATGA T^CATGA
SacI 1217 GAGCTC GAGCT^C
SbfI 1929 CCTGCAGG CCTGCA^GG
SfoI 3 GGCGCC GGC^GCC
SpeI 1588 ACTAGT A^CTAGT
Sse8387I 1929 CCTGCAGG CCTGCA^GG
StuI 1926 AGGCCT AGG^CCT
Van91I 976 CCATATGTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoII 1083 GGATCT [GA]^GATC[CT]
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# TWO CUT ENZYMES ( 35 enzymes) #
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AclI 941 AACGTT AA^CGTT
967 AACGTT
AcyI 3 GGCGCC G[GA]^CG[CT]C
25 GACGTC
AvaII 781 GGTCC G^G[AT]CC
1999 GGTCC
BanII 67 GGGCTC G[GA]GC[CT]^C
1217 GAGCTC
BbsI 28 GTCTTC GAAGAC(2/6)
1151 GTCTTC
BcgI 114 CGATTTCCGTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
2050 CGAGCCTGCTGC
BpmI 1619 CTCCAG CTGGAG(16/14)
1072 CTGGAG
BpuAI 28 GTCTTC GAAGAC(2/6)
1151 GTCTTC
BsaHI 3 GGCGCC G[GA]^CG[CT]C
25 GACGTC
BsaI 704 GGTCTC GGTCTC(1/5)
1843 GGTCTC
BseRI 391 GAGGAG GAGGAG(10/8)
1865 GAGGAG
BsiYI 38 CCCCACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
976 CCATATGTTGG
BslI 38 CCCCACACTGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
976 CCATATGTTGG
BsmFI 282 GGGAC GGGAC(10/14)
782 GTCCC
Bsp1407I 234 TGTACA T^GTACA
1654 TGTACA
BsrDI 924 CATTGC GCAATG(2/0)
1702 GCAATG
BsrGI 234 TGTACA T^GTACA
1654 TGTACA
BssKI 1071 CCTGG ^CC[ACGT]GG
1391 CCTGG
BstNI 1071 CCTGG CC^[AT]GG
1391 CCTGG
BstXI 407 CCATGTTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1031 CCAGCAAAATGG
EcoRII 1071 CCTGG ^CC[AT]GG
1391 CCTGG
Hin1I 3 GGCGCC G[GA]^CG[CT]C
25 GACGTC
MspA1I 58 CAGCTG C[AC]G^C[GT]G
709 CAGCTG
MvaI 1071 CCTGG CC^[AT]GG
1391 CCTGG
NcoI 951 CCATGG C^CATGG
1839 CCATGG
NlaIV 3 GGCGCC GG[ACGT]^[ACGT]CC
781 GGTCCC
PshAI 1587 GACTAGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
1837 GACCATGGTC
Psp1406I 941 AACGTT AA^CGTT
967 AACGTT
PvuII 58 CAGCTG CAG^CTG
709 CAGCTG
ScrFI 1071 CCTGG CC^[ACGT]GG
1391 CCTGG
SmlI 439 CTTAAG C^T[CT][GA]AG
665 CTCAAG
SspBI 234 TGTACA T^GTACA
1654 TGTACA
SspI 419 AATATT AAT^ATT
554 AATATT
TaqI 678 TCGA T^CGA
2049 TCGA
TthHB8I 678 TCGA T^CGA
2049 TCGA
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# 3 OR MORE CUT ENZYMES ( 55 enzymes) #
#########################################
AciI AcsI AfaI AluI ApoI
AspHI BbvI BfaI BmyI BsaJI
BsiHKAI BsmAI Bsp1286I BsrI BstF5I
Cac8I Cfr13I DdeI DpnII DsaI
EarI EcoT14I Fnu4HI FokI HaeIII
HinfI HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MwoI NdeII NlaIII
PleI PstI RsaI SapI Sau3AI
Sau96I SfaNI SfcI StyI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI