Restriction map for
pFN21AE1395
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# NO CUT ENZYMES ( 148 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcyI AflII AflIII AgeI AhdI
AocI Aor51HI ApaI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AviII AvrII BamHI BanI BbeI
BbrPI BbsI BcgI BciVI BclI
BfaI BfrI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BsgI BsiEI
BsiWI BsmAI BsmBI Bsp1407I BspDI
BspEI BspLU11I BspMI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstYI BstZ17I Bsu36I
CelII Cfr10I ClaI CpoI DraI
DrdI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoNI EcoO65I EcoRV
EcoT22I FbaI FseI FspI HgaI
Hin1I HindIII HpaI KasI KpnI
KspI MaeI MaeII MamI MfeI
MflI MluI MroI MunI NaeI
NarI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflFI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI Tth111I TthHB8I XbaI XcmI
XhoI XhoII XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AccII 505 CGCG CG^CG
Alw44I 1033 GTGCAC G^TGCAC
AlwNI 372 CAGGGCCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1033 GTGCAC G^TGCAC
AspHI 1033 GTGCAC G[AT]GC[AT]^C
BalI 665 TGGCCA TGG^CCA
BanII 548 GAGCCC G[GA]GC[CT]^C
BbvCI 344 CCTCAGC CCTCAGC(-5/-2)
BglI 284 GCCTTTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsiHKAI 1033 GTGCAC G[AT]GC[AT]^C
BsmFI 839 GGGAC GGGAC(10/14)
BsmI 223 GAATGC GAATGC(1/-1)
BspHI 734 TCATGA T^CATGA
BsrBI 633 GAGCGG CCGCTC(-3/-3)
BssSI 802 CTCGTG CACGAG(-5/-1)
BstUI 505 CGCG CG^CG
BstXI 722 CCATCCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
DraIII 625 CACGGGGTG CAC[ACGT][ACGT][ACGT]^GTG
EarI 72 CTCTTC CTCTTC(1/4)
EcoRI 816 GAATTC G^AATTC
FauI 503 CCCGC CCCGC(4/6)
HaeII 430 AGCGCC [GA]GCGC^[CT]
HincII 67 GTCAAC GT[CT]^[GA]AC
HindII 67 GTCAAC GT[CT]^[GA]AC
Ksp632I 72 CTCTTC CTCTTC(1/4)
MluNI 665 TGGCCA TGG^CCA
MscI 665 TGGCCA TGG^CCA
MseI 779 TTAA T^TAA
MvnI 505 CGCG CG^CG
NcoI 668 CCATGG C^CATGG
PflMI 198 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PpuMI 296 GGGTCCT [GA]G^G[AT]CC[CT]
PstI 468 CTGCAG CTGCA^G
RcaI 734 TCATGA T^CATGA
SmaI 893 CCCGGG CCC^GGG
StuI 135 AGGCCT AGG^CCT
Tru9I 779 TTAA T^TAA
Van91I 198 CCAGGGCGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 893 CCCGGG C^CCGGG
XmaI 893 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 16 enzymes) #
###################################
AcsI 496 AAATTT [GA]^AATT[CT]
816 GAATTC
ApoI 496 AAATTT [GA]^AATT[CT]
816 GAATTC
AvaI 61 CCCGAG C^[CT]CG[GA]G
893 CCCGGG
AvaII 297 GGTCC G^G[AT]CC
840 GGACC
BmyI 548 GAGCCC G[AGT]GC[ACT]^C
1033 GTGCAC
BseRI 105 CTCCTC GAGGAG(10/8)
342 CTCCTC
BsoBI 61 CCCGAG C^[CT]CG[GA]G
893 CCCGGG
Bsp1286I 548 GAGCCC G[AGT]GC[ACT]^C
1033 GTGCAC
DdeI 345 CTCAG C^T[ACGT]AG
649 CTCAG
DsaI 624 CCACGG C^C[GA][CT]GG
668 CCATGG
HapII 894 CCGG C^CGG
957 CCGG
HpaII 894 CCGG C^CGG
957 CCGG
MaeIII 164 GTCAC ^GT[ACGT]AC
952 GTCAC
MspI 894 CCGG C^CGG
957 CCGG
NspI 655 ACATGC [GA]CATG^[CT]
1039 ACATGC
Tsp45I 164 GTCAC ^GT[GC]AC
952 GTCAC
#########################################
# 3 OR MORE CUT ENZYMES ( 54 enzymes) #
#########################################
AciI AfaI AluI AlwI BbvI
BcnI BpmI BsaJI BsiYI BslI
BsrI BssKI BstF5I BstNI Cac8I
CfoI Cfr13I DpnII DraII EaeI
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HhaI HinP1I HinfI HphI
ItaI MboI MboII MnlI MslI
MspA1I MvaI MwoI NciI NdeII
NlaIII NlaIV PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TfiI TseI Tsp509I TspRI