##################################
# NO CUT ENZYMES (  214 enzymes) #
##################################

AatII       Acc65I      AccI        AccII       AccIII      
AclI        AcsI        AcyI        AflII       AflIII      
AgeI        AhdI        Alw44I      AlwI        AlwNI       
AocI        Aor51HI     ApaI        ApaLI       ApoI        
AscI        AseI        AsnI        Asp700I     Asp718I     
AspEI       AspHI       AspI        AvaI        AviII       
AvrII       BalI        BamHI       BanI        BanII       
BbeI        BbrPI       BbvCI       BcgI        BciVI       
BclI        BcnI        BfaI        BfrI        BglI        
BglII       BlnI        BlpI        BmyI        BpmI        
Bpu1102I    BsaAI       BsaBI       BsaHI       BsaI        
BsaJI       BsaWI       BseAI       BsiEI       BsiHKAI     
BsiWI       BsmBI       BsmI        BsoBI       Bsp1286I    
Bsp1407I    BspDI       BspEI       BspHI       BspLU11I    
BspMI       BsrBI       BsrDI       BsrFI       BsrGI       
BssHII      BssSI       Bst1107I    BstAPI      BstBI       
BstEII      BstF5I      BstPI       BstUI       BstXI       
BstYI       BstZ17I     Bsu36I      Cac8I       CelII       
Cfr10I      ClaI        CpoI        DdeI        DpnII       
DraII       DraIII      DrdI        DsaI        EaeI        
EagI        Eam1105I    EarI        EclXI       Eco47III    
Eco52I      Eco81I      EcoNI       EcoO109I    EcoO65I     
EcoRI       EcoRV       EcoT14I     EcoT22I     FauI        
FbaI        FokI        FseI        FspI        HaeII       
HaeIII      HapII       HgaI        Hin1I       HincII      
HindII      HindIII     HinfI       HpaI        HpaII       
KasI        KpnI        Ksp632I     KspI        MaeI        
MaeII       MamI        MboI        MflI        MluI        
MluNI       MroI        MscI        MslI        MspA1I      
MspI        MvnI        MwoI        NaeI        NarI        
NciI        NcoI        NdeI        NdeII       NgoMIV      
NheI        NlaIV       NotI        NruI        NsiI        
NspI        NspV        PacI        PaeR7I      PflFI       
PflMI       PinAI       PleI        PmaCI       PmeI        
PmlI        PpuMI       PshAI       PshBI       Psp1406I    
PstI        PvuI        PvuII       RcaI        RsrII       
SacI        SacII       SalI        SapI        Sau3AI      
SbfI        ScaI        SexAI       SfiI        SfoI        
SfuI        SgrAI       SmaI        SmlI        SnaBI       
SpeI        SphI        Sse8387I    SspBI       SspI        
StuI        StyI        TaqI        TfiI        Tth111I     
TthHB8I     Van91I      XbaI        XcmI        XhoI        
XhoII       XmaCI       XmaI        XmnI        


###################################
# ONE CUT ENZYMES (   30 enzymes) #
###################################

AciI           19  GCGG                 CCGC(-3/-1)
AfaI          306  GTAC                 GT^AC
AvaII         125  GGACC                G^G[AT]CC
BbsI            4  GAAGAC               GAAGAC(2/6)
BpuAI           4  GAAGAC               GAAGAC(2/6)
BseRI          22  GAGGAG               GAGGAG(10/8)
BsgI          276  CTGCAC               GTGCAG(16/14)
BsmAI          25  GAGAC                GTCTC(1/5)
BsmFI         254  GGGAC                GGGAC(10/14)
BsrI          120  CCAGT                ACTGG(1/-1)
BssKI          12  CCAGG                ^CC[ACGT]GG
BstNI          12  CCAGG                CC^[AT]GG
CfoI           80  GCGC                 GCG^C
Cfr13I        125  GGACC                G^G[ACGT]CC
DraI          181  TTTAAA               TTT^AAA
EcoRII         12  CCAGG                ^CC[AT]GG
HhaI           80  GCGC                 GCG^C
HinP1I         80  GCGC                 G^CGC
HphI          161  TCACC                GGTGA(8/7)
MfeI           83  CAATTG               C^AATTG
MunI           83  CAATTG               C^AATTG
MvaI           12  CCAGG                CC^[AT]GG
NlaIII        303  CATG                 CATG^
RsaI          306  GTAC                 GT^AC
Sau96I        125  GGACC                G^G[ACGT]CC
ScrFI          12  CCAGG                CC^[ACGT]GG
SfaNI         311  GCATC                GCATC(5/9)
SfcI           71  CTATAG               C^T[GA][CT]AG
SwaI          180  ATTTAAAT             ATTT^AAAT
Tsp45I        193  GTGAC                ^GT[GC]AC


###################################
# TWO CUT ENZYMES (   12 enzymes) #
###################################

BbvI           16  GCTGC                GCAGC(8/12)
              275  GCTGC               
BsiYI          12  CCAGGCTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              245  CCTGCTTCTGG         
BslI           12  CCAGGCTGCGG          CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
              245  CCTGCTTCTGG         
Fnu4HI         16  GCTGC                GC^[ACGT]GC
              275  GCTGC               
ItaI           16  GCTGC                GC^[ACGT]GC
              275  GCTGC               
MaeIII         92  GTAAC                ^GT[ACGT]AC
              193  GTGAC               
MboII           4  GAAGA                GAAGA(8/7)
              213  GAAGA               
MnlI           22  GAGG                 CCTC(7/6)
               67  GAGG                
MseI          151  TTAA                 T^TAA
              182  TTAA                
Tru9I         151  TTAA                 T^TAA
              182  TTAA                
TseI           16  GCTGC                G^C[AT]GC
              275  GCTGC               
Tsp509I        84  AATT                 ^AATT
              204  AATT                


#########################################
# 3 OR MORE CUT ENZYMES (    2 enzymes) #
#########################################

AluI        TspRI