Restriction map for
pFN21AE6214
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AlwNI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvrII BbeI BbsI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BlpI Bpu1102I BpuAI BsaBI BseAI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspHI BspLU11I BsrBI BsrDI
BsrGI BsrI Bst1107I BstAPI BstBI
BstF5I BstZ17I CelII ClaI CpoI
DraI DrdI DsaI Eam1105I EarI
EcoNI EcoRV EcoT22I FbaI FokI
FseI HincII HindII HindIII HinfI
HpaI KasI KpnI Ksp632I KspI
MamI MboII MluI MroI MslI
NaeI NarI NcoI NdeI NgoMIV
NheI NruI NsiI NspI NspV
PacI PaeR7I PflFI PflMI PinAI
PleI PmeI PshAI PshBI Psp1406I
PstI PvuI RcaI RsrII SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I Van91I XbaI XcmI XhoI
XmnI
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# ONE CUT ENZYMES ( 57 enzymes) #
###################################
AcsI 231 GAATTC [GA]^AATT[CT]
AcyI 74 GACGCC G[GA]^CG[CT]C
AfaI 777 GTAC GT^AC
AocI 501 CCTGAGG CC^T[ACGT]AGG
Aor51HI 542 AGCGCT AGC^GCT
ApoI 231 GAATTC [GA]^AATT[CT]
AspHI 321 GAGCTC G[AT]GC[AT]^C
AvaI 51 CCCGGG C^[CT]CG[GA]G
BalI 563 TGGCCA TGG^CCA
BamHI 752 GGATCC G^GATCC
BanI 675 GGCACC G^G[CT][GA]CC
BbrPI 460 CACGTG CAC^GTG
BcgI 585 CGAGTTTGCTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfaI 793 CTAG C^TAG
BsaAI 460 CACGTG [CT]AC^GT[GA]
BsaHI 74 GACGCC G[GA]^CG[CT]C
BsaI 5 GGTCTC GGTCTC(1/5)
BseRI 757 CTCCTC GAGGAG(10/8)
BsiEI 41 CGGCCG CG[GA][CT]^CG
BsiHKAI 321 GAGCTC G[AT]GC[AT]^C
BsmAI 6 GTCTC GTCTC(1/5)
BsoBI 51 CCCGGG C^[CT]CG[GA]G
BsrFI 550 ACCGGC [GA]^CCGG[CT]
BssSI 340 CTCGTG CACGAG(-5/-1)
BstEII 495 GGTGACC G^GT[ACGT]ACC
BstPI 495 GGTGACC G^GT[ACGT]ACC
BstXI 713 CCACAGCTTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 501 CCTGAGG CC^T[ACGT]AGG
Cfr10I 550 ACCGGC [GA]^CCGG[CT]
DraIII 403 CACAGCGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 41 CGGCCG C^GGCCG
EclXI 41 CGGCCG C^GGCCG
Eco47III 542 AGCGCT AGC^GCT
Eco52I 41 CGGCCG C^GGCCG
Eco81I 501 CCTGAGG CC^T[ACGT]AGG
EcoO65I 495 GGTGACC G^GT[ACGT]ACC
EcoRI 231 GAATTC G^AATTC
EcoT14I 101 CCAAGG C^C[AT][AT]GG
Hin1I 74 GACGCC G[GA]^CG[CT]C
MaeI 793 CTAG C^TAG
MfeI 201 CAATTG C^AATTG
MluNI 563 TGGCCA TGG^CCA
MscI 563 TGGCCA TGG^CCA
MseI 430 TTAA T^TAA
MunI 201 CAATTG C^AATTG
NotI 40 GCGGCCGC GC^GGCCGC
PmaCI 460 CACGTG CAC^GTG
PmlI 460 CACGTG CAC^GTG
PpuMI 127 GGGACCT [GA]G^G[AT]CC[CT]
RsaI 777 GTAC GT^AC
SacI 321 GAGCTC GAGCT^C
SfaNI 690 GATGC GCATC(5/9)
SmaI 51 CCCGGG CCC^GGG
StyI 101 CCAAGG C^C[AT][AT]GG
Tru9I 430 TTAA T^TAA
XmaCI 51 CCCGGG C^CCGGG
XmaI 51 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AviII 149 TGCGCA TGC^GCA
349 TGCGCA
BsaWI 423 TCCGGT [AT]^CCGG[AT]
727 TCCGGT
BsgI 175 GTGCAG GTGCAG(16/14)
193 GTGCAG
BspMI 346 ACCTGC ACCTGC(4/8)
468 GCAGGT
BssHII 65 GCGCGC G^CGCGC
634 GCGCGC
BstYI 558 GGATCT [GA]^GATC[CT]
752 GGATCC
DraII 127 GGGACCT [GA]G^G[ACGT]CC[CT]
313 AGGGCCT
EaeI 41 CGGCCG [CT]^GGCC[GA]
563 TGGCCA
EcoO109I 127 GGGACCT [GA]G^G[ACGT]CC[CT]
313 AGGGCCT
FspI 149 TGCGCA TGC^GCA
349 TGCGCA
HaeII 542 AGCGCT [GA]GCGC^[CT]
769 GGCGCT
MflI 558 GGATCT [GA]^GATC[CT]
752 GGATCC
NlaIII 153 CATG CATG^
508 CATG
PvuII 213 CAGCTG CAG^CTG
298 CAGCTG
TaqI 305 TCGA T^CGA
647 TCGA
Tsp509I 202 AATT ^AATT
232 AATT
TthHB8I 305 TCGA T^CGA
647 TCGA
XhoII 558 GGATCT [GA]^GATC[CT]
752 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 52 enzymes) #
#########################################
AccII AciI AluI AlwI AvaII
BanII BbvI BcnI BmyI BpmI
BsaJI BsiYI BslI BsmFI Bsp1286I
BssKI BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII EcoRII FauI
Fnu4HI HaeIII HapII HgaI HhaI
HinP1I HpaII HphI ItaI MaeII
MaeIII MboI MnlI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIV Sau3AI Sau96I ScrFI TseI
Tsp45I TspRI