Restriction map for
pFN21AE3929
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# NO CUT ENZYMES ( 112 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AflII AflIII AgeI AhdI Alw44I
AocI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AvrII BalI
BamHI BbeI BbrPI BbvCI BciVI
BclI BfrI BglI BglII BlnI
BsaAI BsaI BsaWI BseAI BseRI
BsiWI BsmAI BsmBI Bsp1407I BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstZ17I Bsu36I CpoI DraI
DrdI Eam1105I Eco81I EcoO65I EcoRV
EcoT22I FbaI FseI KasI KpnI
KspI MfeI MluI MluNI MroI
MscI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PinAI PmaCI PmeI PmlI PshBI
Psp1406I RcaI RsrII SacII SalI
SapI SexAI SfiI SfoI SfuI
SgrAI SnaBI SpeI SspBI SspI
StuI SwaI Tsp45I Van91I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AcsI 1247 GAATTC [GA]^AATT[CT]
AcyI 637 GGCGTC G[GA]^CG[CT]C
Aor51HI 18 AGCGCT AGC^GCT
ApaI 236 GGGCCC GGGCC^C
ApoI 1247 GAATTC [GA]^AATT[CT]
AspI 560 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 959 TGCGCA TGC^GCA
BanI 819 GGTGCC G^G[CT][GA]CC
BcgI 140 GCAGCCGCTTCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BsaBI 1008 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 637 GGCGTC G[GA]^CG[CT]C
BsgI 340 CTGCAC GTGCAG(16/14)
BsmI 1109 GCATTC GAATGC(1/-1)
BspMI 713 ACCTGC ACCTGC(4/8)
BsrFI 355 ACCGGC [GA]^CCGG[CT]
BssHII 386 GCGCGC G^CGCGC
BstF5I 1007 GGATG GGATG(2/0)
Cfr10I 355 ACCGGC [GA]^CCGG[CT]
DraIII 1017 CACCCAGTG CAC[ACGT][ACGT][ACGT]^GTG
EagI 357 CGGCCG C^GGCCG
EarI 866 CTCTTC CTCTTC(1/4)
EclXI 357 CGGCCG C^GGCCG
Eco47III 18 AGCGCT AGC^GCT
Eco52I 357 CGGCCG C^GGCCG
EcoRI 1247 GAATTC G^AATTC
FokI 1007 GGATG GGATG(9/13)
FspI 959 TGCGCA TGC^GCA
HgaI 638 GCGTC GACGC(5/10)
Hin1I 637 GGCGTC G[GA]^CG[CT]C
HindIII 1357 AAGCTT A^AGCTT
HpaI 1396 GTTAAC GTT^AAC
Ksp632I 866 CTCTTC CTCTTC(1/4)
MaeIII 1025 GTTAC ^GT[ACGT]AC
MamI 1008 GATGACAATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
PflFI 560 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PpuMI 558 AGGACCT [GA]G^G[AT]CC[CT]
PshAI 796 GACAGGTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PvuI 1284 CGATCG CGAT^CG
SacI 430 GAGCTC GAGCT^C
SbfI 399 CCTGCAGG CCTGCA^GG
SmaI 232 CCCGGG CCC^GGG
SphI 962 GCATGC GCATG^C
Sse8387I 399 CCTGCAGG CCTGCA^GG
Tsp509I 1248 AATT ^AATT
Tth111I 560 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XmaCI 232 CCCGGG C^CCGGG
XmaI 232 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 28 enzymes) #
###################################
AccII 188 CGCG CG^CG
387 CGCG
AlwI 408 GATCC GGATC(4/5)
610 GGATC
AspHI 151 GTGCTC G[AT]GC[AT]^C
430 GAGCTC
AvaI 109 CTCGGG C^[CT]CG[GA]G
232 CCCGGG
AvaII 559 GGACC G^G[AT]CC
711 GGACC
BbsI 514 GAAGAC GAAGAC(2/6)
73 GTCTTC
BfaI 68 CTAG C^TAG
1469 CTAG
BlpI 432 GCTCAGC GC^T[ACGT]AGC
1099 GCTGAGC
Bpu1102I 432 GCTCAGC GC^T[ACGT]AGC
1099 GCTGAGC
BpuAI 514 GAAGAC GAAGAC(2/6)
73 GTCTTC
BsiHKAI 151 GTGCTC G[AT]GC[AT]^C
430 GAGCTC
BsoBI 109 CTCGGG C^[CT]CG[GA]G
232 CCCGGG
BspDI 424 ATCGAT AT^CGAT
439 ATCGAT
BstUI 188 CGCG CG^CG
387 CGCG
BstXI 254 CCAGCCCTCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
281 CCAACCCCGTGG
BstYI 407 AGATCC [GA]^GATC[CT]
610 GGATCT
CelII 432 GCTCAGC GC^T[ACGT]AGC
1099 GCTGAGC
ClaI 424 ATCGAT AT^CGAT
439 ATCGAT
EcoNI 446 CCTGCTGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
810 CCTAAGAAAGG
MaeI 68 CTAG C^TAG
1469 CTAG
MaeII 131 ACGT A^CGT
917 ACGT
MflI 407 AGATCC [GA]^GATC[CT]
610 GGATCT
MvnI 188 CGCG CG^CG
387 CGCG
NspI 366 GCATGT [GA]CATG^[CT]
962 GCATGC
PleI 28 GACTC GAGTC(4/5)
1381 GACTC
SmlI 1318 CTCAAG C^T[CT][GA]AG
1401 CTTGAG
TfiI 442 GATTC G^A[AT]TC
1067 GAATC
XhoII 407 AGATCC [GA]^GATC[CT]
610 GGATCT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AciI AfaI AluI AlwNI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiEI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstNI Cac8I CfoI
Cfr13I DdeI DpnII DraII DsaI
EaeI EcoO109I EcoRII EcoT14I FauI
Fnu4HI HaeII HaeIII HapII HhaI
HinP1I HincII HindII HinfI HpaII
HphI ItaI MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NdeII NlaIII NlaIV
PstI PvuII RsaI Sau3AI Sau96I
ScaI ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI TspRI TthHB8I
XcmI