Restriction map for
pFN21AE3947
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# NO CUT ENZYMES ( 104 enzymes) #
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AatII Acc65I AccII AccIII AciI
AclI AflII AgeI AhdI ApaI
AscI Asp700I Asp718I AspEI AvaI
AviII AvrII BbeI BbrPI BbvCI
BcgI BclI BfrI BglI BlnI
BsaBI BsaI BseAI BsiEI BsiWI
BsmBI BsoBI BspDI BspEI BsrBI
BsrFI BssHII BssSI Bst1107I BstEII
BstPI BstUI BstZ17I Cfr10I ClaI
CpoI DraII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO109I EcoO65I
EcoRI FauI FbaI FseI FspI
HpaI KasI KpnI KspI MamI
MfeI MluI MroI MspA1I MunI
MvnI NaeI NarI NdeI NgoMIV
NheI NotI NruI PacI PaeR7I
PinAI PmaCI PmeI PmlI PpuMI
PshAI Psp1406I PvuI PvuII RsrII
SacII SalI ScaI SfiI SfoI
SgrAI SmaI SphI StuI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccI 3205 GTCTAC GT^[AC][GT]AC
Alw44I 1076 GTGCAC G^TGCAC
Aor51HI 2680 AGCGCT AGC^GCT
ApaLI 1076 GTGCAC G^TGCAC
AspI 543 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 876 TGGCCA TGG^CCA
BamHI 113 GGATCC G^GATCC
BanI 2010 GGTGCC G^G[CT][GA]CC
BbsI 799 GAAGAC GAAGAC(2/6)
BciVI 933 GGATAC GTATCC(6/5)
BlpI 250 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 250 GCTCAGC GC^T[ACGT]AGC
BpuAI 799 GAAGAC GAAGAC(2/6)
BsaAI 2048 TACGTA [CT]AC^GT[GA]
BsaWI 3191 ACCGGA [AT]^CCGG[AT]
BsmI 511 GCATTC GAATGC(1/-1)
BspHI 3249 TCATGA T^CATGA
BstBI 3119 TTCGAA TT^CGAA
BstXI 2948 CCAATGGACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 250 GCTCAGC GC^T[ACGT]AGC
Eco47III 2680 AGCGCT AGC^GCT
EcoT22I 601 ATGCAT ATGCA^T
MaeII 2049 ACGT A^CGT
MluNI 876 TGGCCA TGG^CCA
MscI 876 TGGCCA TGG^CCA
NsiI 601 ATGCAT ATGCA^T
NspV 3119 TTCGAA TT^CGAA
PflFI 543 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 958 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 3249 TCATGA T^CATGA
SacI 2245 GAGCTC GAGCT^C
SapI 2857 GAAGAGC GCTCTTC(1/4)
SbfI 1938 CCTGCAGG CCTGCA^GG
SexAI 963 ACCTGGT A^CC[AT]GGT
SfuI 3119 TTCGAA TT^CGAA
SmlI 609 CTCAAG C^T[CT][GA]AG
SnaBI 2048 TACGTA TAC^GTA
SpeI 190 ACTAGT A^CTAGT
Sse8387I 1938 CCTGCAGG CCTGCA^GG
SwaI 641 ATTTAAAT ATTT^AAAT
TaqI 3120 TCGA T^CGA
Tth111I 543 GACCATGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 3120 TCGA T^CGA
Van91I 958 CCACTACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XbaI 342 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 26 enzymes) #
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AflIII 2445 ACATGT A^C[GA][CT]GT
3029 ACATGT
AseI 1476 ATTAAT AT^TAAT
2494 ATTAAT
AsnI 1476 ATTAAT AT^TAAT
2494 ATTAAT
AspHI 1076 GTGCAC G[AT]GC[AT]^C
2245 GAGCTC
BanII 2189 GGGCTC G[GA]GC[CT]^C
2245 GAGCTC
BseRI 1573 GAGGAG GAGGAG(10/8)
3100 GAGGAG
BsgI 228 CTGCAC GTGCAG(16/14)
3157 GTGCAG
BsiHKAI 1076 GTGCAC G[AT]GC[AT]^C
2245 GAGCTC
Bsp1407I 1103 TGTACA T^GTACA
1955 TGTACA
BspLU11I 2445 ACATGT A^CATGT
3029 ACATGT
BsrGI 1103 TGTACA T^GTACA
1955 TGTACA
BstAPI 241 GCAAGAATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2919 GCACAGGGTGC
CfoI 2681 GCGC GCG^C
2862 GCGC
DraIII 1259 CACATGGTG CAC[ACGT][ACGT][ACGT]^GTG
2920 CACAGGGTG
DsaI 221 CCATGG C^C[GA][CT]GG
2663 CCATGG
EaeI 876 TGGCCA [CT]^GGCC[GA]
1559 TGGCCG
HaeII 2680 AGCGCT [GA]GCGC^[CT]
2861 AGCGCC
HhaI 2681 GCGC GCG^C
2862 GCGC
HinP1I 2681 GCGC G^CGC
2862 GCGC
HindIII 766 AAGCTT A^AGCTT
1732 AAGCTT
NcoI 221 CCATGG C^CATGG
2663 CCATGG
PleI 2212 GACTC GAGTC(4/5)
2572 GACTC
PshBI 1476 ATTAAT AT^TAAT
2494 ATTAAT
PstI 43 CTGCAG CTGCA^G
1939 CTGCAG
SspBI 1103 TGTACA T^GTACA
1955 TGTACA
SspI 1499 AATATT AAT^ATT
1978 AATATT
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# 3 OR MORE CUT ENZYMES ( 83 enzymes) #
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AcsI AcyI AfaI AluI AlwI
AlwNI AocI ApoI AvaII BbvI
BcnI BfaI BglII BmyI BpmI
BsaHI BsaJI BsiYI BslI BsmAI
BsmFI Bsp1286I BspMI BsrDI BsrI
BssKI BstF5I BstNI BstYI Bsu36I
Cac8I Cfr13I DdeI DpnII DraI
EarI Eco81I EcoRII EcoRV EcoT14I
Fnu4HI FokI HaeIII HapII HgaI
Hin1I HincII HindII HinfI HpaII
HphI ItaI Ksp632I MaeI MaeIII
MboI MboII MflI MnlI MseI
MslI MspI MvaI MwoI NciI
NdeII NlaIII NlaIV NspI RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
StyI TfiI Tru9I TseI Tsp45I
Tsp509I TspRI XhoII