Restriction map for
pFN21AE3674
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# NO CUT ENZYMES ( 137 enzymes) #
##################################
AatII AccII AccIII AclI AflII
AflIII AhdI Alw44I AlwNI AocI
ApaI ApaLI AscI AseI AsnI
AspEI AspHI AviII AvrII BalI
BamHI BanII BbrPI BbsI BbvCI
BcgI BciVI BclI BfrI BglI
BlnI BlpI BmyI Bpu1102I BpuAI
BsaAI BsaI BseAI BseRI BsgI
BsiEI BsiHKAI BsiWI BsmBI Bsp1286I
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrDI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraIII EagI Eam1105I
EclXI Eco52I Eco81I EcoO65I EcoRI
EcoRV EcoT22I FauI FbaI FseI
FspI HgaI HincII HindII HindIII
HpaI KspI MfeI MluI MluNI
MroI MscI MunI MvnI NaeI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflMI
PmaCI PmeI PmlI PshAI PshBI
Psp1406I PvuI RcaI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfuI SgrAI SmaI SmlI
SnaBI SpeI SphI Sse8387I SspBI
SwaI Tsp45I Van91I XbaI XhoI
XmaCI XmaI
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# ONE CUT ENZYMES ( 52 enzymes) #
###################################
Acc65I 626 GGTACC G^GTACC
AccI 742 GTAGAC GT^[AC][GT]AC
AciI 350 CCGC CCGC(-3/-1)
AcyI 832 GGCGCC G[GA]^CG[CT]C
AgeI 178 ACCGGT A^CCGGT
AlwI 430 GATCC GGATC(4/5)
Aor51HI 302 AGCGCT AGC^GCT
Asp700I 514 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 626 GGTACC G^GTACC
AspI 745 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 167 CTCGGG C^[CT]CG[GA]G
AvaII 240 GGACC G^G[AT]CC
BbeI 832 GGCGCC GGCGC^C
BcnI 74 CCGGG CC^[GC]GG
BglII 807 AGATCT A^GATCT
BpmI 566 CTGGAG CTGGAG(16/14)
BsaBI 901 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 832 GGCGCC G[GA]^CG[CT]C
BsaWI 178 ACCGGT [AT]^CCGG[AT]
BsmAI 606 GTCTC GTCTC(1/5)
BsmI 981 GAATGC GAATGC(1/-1)
BsoBI 167 CTCGGG C^[CT]CG[GA]G
BsrFI 178 ACCGGT [GA]^CCGG[CT]
BstYI 807 AGATCT [GA]^GATC[CT]
Cfr10I 178 ACCGGT [GA]^CCGG[CT]
DrdI 1153 GACCTGCTAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 302 AGCGCT AGC^GCT
EcoNI 1011 CCTCACACAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
Hin1I 832 GGCGCC G[GA]^CG[CT]C
KasI 832 GGCGCC G^GCGCC
KpnI 626 GGTACC GGTAC^C
MaeII 749 ACGT A^CGT
MamI 901 GATGCCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MflI 807 AGATCT [GA]^GATC[CT]
NarI 832 GGCGCC GG^CGCC
NciI 74 CCGGG CC^[GC]GG
NcoI 828 CCATGG C^CATGG
NspI 851 ACATGC [GA]CATG^[CT]
PflFI 745 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
PinAI 178 ACCGGT A^CCGGT
PleI 84 GACTC GAGTC(4/5)
PpuMI 239 GGGACCC [GA]G^G[AT]CC[CT]
PstI 974 CTGCAG CTGCA^G
PvuII 757 CAGCTG CAG^CTG
SapI 481 GCTCTTC GCTCTTC(1/4)
SfoI 832 GGCGCC GGC^GCC
SspI 635 AATATT AAT^ATT
TaqI 804 TCGA T^CGA
Tth111I 745 GACTACGTC GAC[ACGT]^[ACGT][ACGT]GTC
TthHB8I 804 TCGA T^CGA
XhoII 807 AGATCT [GA]^GATC[CT]
XmnI 514 GAAAAGATTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 30 enzymes) #
###################################
AcsI 668 GAATTT [GA]^AATT[CT]
763 AAATTC
AfaI 627 GTAC GT^AC
1029 GTAC
ApoI 668 GAATTT [GA]^AATT[CT]
763 AAATTC
BsmFI 239 GGGAC GGGAC(10/14)
1162 GTCCC
BspMI 1154 ACCTGC ACCTGC(4/8)
196 GCAGGT
BsrI 187 ACTGG ACTGG(1/-1)
737 CCAGT
BstAPI 196 GCAGGTATTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
976 GCAGGGAATGC
CfoI 303 GCGC GCG^C
833 GCGC
DdeI 89 CTGAG C^T[ACGT]AG
1065 CTTAG
DsaI 828 CCATGG C^C[GA][CT]GG
966 CCACGG
EaeI 50 TGGCCG [CT]^GGCC[GA]
347 TGGCCG
EarI 482 CTCTTC CTCTTC(1/4)
585 GAAGAG
EcoT14I 708 CCAAGG C^C[AT][AT]GG
828 CCATGG
HaeII 302 AGCGCT [GA]GCGC^[CT]
832 GGCGCC
HapII 74 CCGG C^CGG
179 CCGG
HhaI 303 GCGC GCG^C
833 GCGC
HinP1I 303 GCGC G^CGC
833 GCGC
HpaII 74 CCGG C^CGG
179 CCGG
Ksp632I 482 CTCTTC CTCTTC(1/4)
585 GAAGAG
MaeIII 182 GTTAC ^GT[ACGT]AC
717 GTTAC
MseI 295 TTAA T^TAA
559 TTAA
MslI 220 CATCTTTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
265 CATGGCTATG
MspA1I 350 CCGCTG C[AC]G^C[GT]G
757 CAGCTG
MspI 74 CCGG C^CGG
179 CCGG
RsaI 627 GTAC GT^AC
1029 GTAC
SfcI 253 CTACAG C^T[GA][CT]AG
974 CTGCAG
StuI 147 AGGCCT AGG^CCT
863 AGGCCT
StyI 708 CCAAGG C^C[AT][AT]GG
828 CCATGG
Tru9I 295 TTAA T^TAA
559 TTAA
XcmI 819 CCATTGGTGCCATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
828 CCATGGCGCCCCTGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AluI BanI BbvI BfaI BsaJI
BsiYI BslI BssKI BstF5I BstNI
Cac8I Cfr13I DpnII DraII EcoO109I
EcoRII Fnu4HI FokI HaeIII HinfI
HphI ItaI MaeI MboI MboII
MnlI MvaI MwoI NdeII NlaIII
NlaIV Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp509I TspRI